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AB114648

Recombinant Human Cytochrome P450 2B6/CYP2B6 protein

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Recombinant Human Cytochrome P450 2B6/CYP2B6 protein is a Human Full Length protein, in the 1 to 491 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

Cytochrome P450 2B6, CYPIIB6, Cytochrome P450 IIB1, CYP2B6

1 Images
SDS-PAGE - Recombinant Human Cytochrome P450 2B6/CYP2B6 protein (AB114648)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Cytochrome P450 2B6/CYP2B6 protein (AB114648)

ab114648 analysed on a 12.5% SDS-PAGE gel stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

Tag free

Applications

SDS-PAGE, ELISA, WB

applications

Biologically active

No

Accession

P20813

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.3% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as Cytochrome P450 2B6.
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Sequence info

[{"sequence":"MELSVLLFLALLTGLLLLLVQRHPNTHDRLPPGPRPLPLLGNLLQMDRRGLLKSFLRFREKYGDVFTVHLGPRPVVMLCGVEAIREALVDKAEAFSGRGKIAMVDPFFRGYGVIFANGNRWKVLRRFSVTTMRDFGMGKRSVEERIQEEAQCLIEELRKSKGALMDPTFLFQSITANIICSIVFGKRFHYQDQEFLKMLNLFYQTFSLISSVFGQLFELFSGFLTYFPGAHRQVYKNLQEINAYIGHSVEKHRETLDPSAPKDLIDTYLLHMEEEKSNAHSEFSHQNLNLNTLSLFFAGTETTSTTLRYGFLLMLKYPHVAERVYREIEQVIGPHRPPELHDRAKMPYTEAVIYEIQRFSDLLPMGVPHIVTQHTSFRGYIIPKDTEVFLILSTALHDPHYFEKPDAFNPDHFLDANGALKKTEAFIPFSLGKRICLGEGIARAELFLFFTTILQNFSMASPVAPEDIDLTPQECGVGKIPPTYQIRFLPR","proteinLength":"Full Length","predictedMolecularWeight":"80.08 kDa","actualMolecularWeight":null,"aminoAcidEnd":491,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P20813","tags":[]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytochrome P450 2B6 (CYP2B6) an essential enzyme in the cytochrome P450 superfamily plays a role in the metabolism of various substances. Often referred to as CYP2B6 or P450 2B6 this enzyme has a molecular mass of approximately 56 kDa. CYP2B6 is predominantly expressed in the liver but also found in the brain and testes. It catalyzes oxidation reactions thereby affecting the biotransformation of endogenous and exogenous compounds including drugs and steroids.
Biological function summary

CYP2B6 is involved in metabolizing drugs and synthesizing cholesterol steroids and other lipids. It forms part of a broader enzymatic complex that facilitates these biochemical reactions. As a monooxygenase it introduces an oxygen atom into its substrates contributing to drug metabolism and clearance from the body. This function aids detoxification processes and supports homeostasis.

Pathways

CYP2B6 is integral to the metabolism of xenobiotics and drug biotransformation pathways. It closely interacts with other cytochrome P450 enzymes such as CYP3A4 in these pathways. During drug metabolism these enzymes can affect the pharmacokinetics and pharmacodynamics of various pharmaceutical agents influencing their efficacy and toxicity.

CYP2B6 shows a connection with drug-induced liver injury and certain neurological disorders. Genetic polymorphisms in CYP2B6 can affect its function leading to variability in drug metabolism between individuals. This can contribute to adverse drug reactions or altered therapeutic effects. The enzyme's interaction with proteins like CYP2D6 relates to altered responses to antidepressants impacting treatment outcomes.
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Specifications

Form

Liquid

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General info

Function

A cytochrome P450 monooxygenase involved in the metabolism of endocannabinoids and steroids (PubMed : 12865317, PubMed : 21289075). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the epoxidation of double bonds of arachidonoylethanolamide (anandamide) to 8,9-, 11,12-, and 14,15-epoxyeicosatrienoic acid ethanolamides (EpETrE-EAs), potentially modulating endocannabinoid system signaling (PubMed : 21289075). Hydroxylates steroid hormones, including testosterone at C-16 and estrogens at C-2 (PubMed : 12865317, PubMed : 21289075). Plays a role in the oxidative metabolism of xenobiotics, including plant lipids and drugs (PubMed : 11695850, PubMed : 22909231). Acts as a 1,4-cineole 2-exo-monooxygenase (PubMed : 11695850).. Allele 2B6*9 : Has low affinity for anandamide and can only produce 11,12 EpETrE-EAs.

Sequence similarities

Belongs to the cytochrome P450 family.

Post-translational modifications

Phosphorylation is accompanied by a decrease in enzyme activity.

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Product protocols

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Target data

A cytochrome P450 monooxygenase involved in the metabolism of endocannabinoids and steroids (PubMed : 12865317, PubMed : 21289075). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the epoxidation of double bonds of arachidonoylethanolamide (anandamide) to 8,9-, 11,12-, and 14,15-epoxyeicosatrienoic acid ethanolamides (EpETrE-EAs), potentially modulating endocannabinoid system signaling (PubMed : 21289075). Hydroxylates steroid hormones, including testosterone at C-16 and estrogens at C-2 (PubMed : 12865317, PubMed : 21289075). Plays a role in the oxidative metabolism of xenobiotics, including plant lipids and drugs (PubMed : 11695850, PubMed : 22909231). Acts as a 1,4-cineole 2-exo-monooxygenase (PubMed : 11695850).. Allele 2B6*9 : Has low affinity for anandamide and can only produce 11,12 EpETrE-EAs.
See full target information Cytochrome P450 2B6
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Complementary Products

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Primary Antibodies

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Primary Antibodies

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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