JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB202203

Recombinant Human Cytochrome P450 2E1 protein (denatured) (His tag N-Terminus)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human Cytochrome P450 2E1 protein (denatured) (His tag N-Terminus) is a Human Fragment protein, in the 29 to 493 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

CYP2E, CYP2E1, Cytochrome P450 2E1, 4-nitrophenol 2-hydroxylase, CYPIIE1, Cytochrome P450-J

1 Images
SDS-PAGE - Recombinant Human Cytochrome P450 2E1 protein (denatured) (His tag N-Terminus) (AB202203)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Cytochrome P450 2E1 protein (denatured) (His tag N-Terminus) (AB202203)

15% SDS-PAGE analysis of ab202203 (3 μg)

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P05181

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.32% Tris HCl

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSSWNLPPGPFPLPIIGNLFQLELKNIPKSFTRLAQRFGPVFTLYVGSQRMVVMHGYKAVKEALLDYKDEFSGRGDLPAFHAHRDRGIIFNNGPTWKDIRRFSLTTLRNYGMGKQGNESRIQREAHFLLEALRKTQGQPFDPTFLIGCAPCNVIADILFRKHFDYNDEKFLRLMYLFNENFHLLSTPWLQLYNNFPSFLHYLPGSHRKVIKNVAEVKEYVSERVKEHHQSLDPNCPRDLTDCLLVEMEKEKHSAERLYTMDGITVTVADLFFAGTETTSTTLRYGLLILMKYPEIEEKLHEEIDRVIGPSRIPAIKDRQEMPYMDAVVHEIQRFITLVPSNLPHEATRDTIFRGYLIPKGTVVVPTLDSVLYDNQEFPDPEKFKPEHFLNENGKFKYSDYFKPFSTGKRVCAGEGLARMELFLLLCAILQHFNLKPLVDPKDIDLSPIHIGFGCIPPRYKLCVIPRS","proteinLength":"Fragment","predictedMolecularWeight":"56.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":493,"aminoAcidStart":29,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P05181","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytochrome P450 2E1 also known as CYP2E1 is an enzyme belonging to the cytochrome P450 superfamily. It weighs approximately 56 kDa. This enzyme is majorly expressed in the liver but can also be found in other tissues like the kidney and lungs. It plays a central role in the oxidation and metabolism of various endogenous and exogenous substances including fatty acids drugs and alcohols.
Biological function summary

The enzyme CYP2E1 metabolizes low-molecular-weight compounds. It catalyzes the conversion of substrates to more soluble forms enabling their excretion from the body. It is not a part of a larger protein complex but often partners with NADPH-cytochrome P450 reductase in electron transfer. CYP2E1 can also produce reactive oxygen species during its catalytic cycle which may impact cellular structures and processes.

Pathways

CYP2E1 is involved in the oxidative metabolism of ethanol and other xenobiotics. It participates in the metabolic pathways for drugs and carcinogens transforming them into either active or inactive metabolites. The enzyme has connections with other P450 enzymes such as CYP3A4 within the same metabolic landscape often overlapping substrates and regulatory mechanisms.

CYP2E1 plays a significant role in alcohol-induced liver injury because its metabolism of ethanol generates toxic by-products like acetaldehyde and reactive oxygen species contributing to liver damage. The enzyme is also associated with the development of certain cancers as its metabolism of procarcinogens can produce carcinogenic metabolites. Other related cytochrome P450 enzymes such as CYP1A2 share pathways involved in carcinogen activation highlighting an interconnected network contributing to disease progression.
style
left-column

Specifications

Form

Liquid

Additional notes

ab202203 was purified using conventional chromatography.

style
left-column

General info

Function

A cytochrome P450 monooxygenase involved in the metabolism of fatty acids (PubMed : 10553002, PubMed : 18577768). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (PubMed : 10553002, PubMed : 18577768). Catalyzes the hydroxylation of carbon-hydrogen bonds. Hydroxylates fatty acids specifically at the omega-1 position displaying the highest catalytic activity for saturated fatty acids (PubMed : 10553002, PubMed : 18577768). May be involved in the oxidative metabolism of xenobiotics (Probable).

Sequence similarities

Belongs to the cytochrome P450 family.

Subcellular localisation

Mitochondrion inner membrane

style
left-column

Product protocols

style
left-column

Target data

A cytochrome P450 monooxygenase involved in the metabolism of fatty acids (PubMed : 10553002, PubMed : 18577768). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (PubMed : 10553002, PubMed : 18577768). Catalyzes the hydroxylation of carbon-hydrogen bonds. Hydroxylates fatty acids specifically at the omega-1 position displaying the highest catalytic activity for saturated fatty acids (PubMed : 10553002, PubMed : 18577768). May be involved in the oxidative metabolism of xenobiotics (Probable).
See full target information CYP2E1
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB28146

Anti-Cytochrome P450 2E1 antibody

Western blot - Anti-Cytochrome P450 2E1 antibody (AB28146)

  • 5
  • 6
Cell Lines & Lysates

AB94254

Cytochrome P450 2E1 overexpression 293T lysate (whole cell)

Western blot - Cytochrome P450 2E1 overexpression 293T lysate (whole cell) (AB94254)

  • 0
  • 0
Primary Antibodies

AB180597

Anti-Cytochrome P450 Reductase antibody [EPR14479(B)]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 Reductase antibody [EPR14479(B)] (AB180597)

  • 0
  • 0
Primary Antibodies

AB151728

Anti-Cytochrome P450 1A2 antibody [EPR6138(2)]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 1A2 antibody [EPR6138(2)] (AB151728)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com