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AB151823

Recombinant Human Cytochrome P450 Reductase protein

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Recombinant Human Cytochrome P450 Reductase protein is a Human Full Length protein, in the 1 to 679 aa range, expressed in Baculovirus infected Sf9 cells, with >95%, suitable for SDS-PAGE, WB.

View Alternative Names

CYPOR, POR, NADPH--cytochrome P450 reductase, CPR, P450R

1 Images
SDS-PAGE - Recombinant Human Cytochrome P450 Reductase protein (AB151823)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Cytochrome P450 Reductase protein (AB151823)

SDS-PAGE analysis of ab151823 which was determined to be >95% pure by densitometry.

Key facts

Purity

>95% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

WB, SDS-PAGE

applications

Biologically active

No

Accession

P16435

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.79% Tris HCl, 0.31% Glutathione, 0.29% Sodium chloride, 0.04% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"INMGDSHVDTSSTVSEAVAEEVSLFSMTDMILFSLIVGLLTYWFLFRKKKEEVPEFTKIQTLTSSVRESSFVEKMKKTGRNIIVFYGSQTGTAEEFANRLSKDAHRYGMRGMSADPEEYDLADLSSLPEIDNALVVFCMATYGEGDPTDNAQDFYDWLQETDVDLSGVKFAVFGLGNKTYEHFNAMGKYVDKRLEQLGAQRIFELGLGDDDGNLEEDFITWREQFWLAVCEHFGVEATGEESSIRQYELVVHTDIDAAKVYMGEMGRLKSYENQKPPFDAKNPFLAAVTTNRKLNQGTERHLMHLELDISDSKIRYESGDHVAVYPANDSALVNQLGKILGADLDVVMSLNNLDEESNKKHPFPCPTSYRTALTYYLDITNPPRTNVLYELAQYASEPSEQELLRKMASSSGEGKELYLSWVVEARRHILAILQDCPSLRPPIDHLCELLPRLQARYYSIASSSKVHPNSVHICAVVVEYETKAGRINKGVATNWLRAKEPVGENGGRALVPMFVRKSQFRLPFKATTPVIMVGPGTGVAPFIGFIQERAWLRQQGKEVGETLLYYGCRRSDEDYLYREELAQFHRDGALTQLNVAFSREQSHKVYVQHLLKQDREHLWKLIEGGAHIYVCGDARNMARDVQNTFYDIVAELGAMEHAQAVDYIKKLMTKGRYSLDVWS","proteinLength":"Full Length","predictedMolecularWeight":"102 kDa","actualMolecularWeight":null,"aminoAcidEnd":679,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"P16435","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytochrome P450 reductase also known as NADPH--cytochrome P450 reductase (CPR) is an enzyme important in electron transfer. It has a mass of approximately 77 kDa and is responsible for transferring electrons from NADPH to cytochrome P450 enzymes supporting their function. The enzyme is predominantly expressed in the endoplasmic reticulum of liver cells and other tissues where detoxification processes occur. Cytochrome reductase as it is sometimes called plays a fundamental role in the metabolism of drugs and the synthesis of cholesterol steroids and other lipids.
Biological function summary

CPR facilitates electron donation to all microsomal cytochrome P450 enzymes which are involved in a variety of oxidative reactions. This protein is essential for P450 activity assay as it is part of a multienzyme complex that metabolizes exogenous and endogenous compounds efficiently. Without cytochrome P450 reductase the catalytic activity of cytochrome P450 enzymes becomes impaired affecting metabolizing mechanisms for various substances and having systemic biological impacts.

Pathways

Cytochrome P450 reductase directly participates in drug metabolism and steroid biosynthesis pathways. It together with the cytochrome P450 enzymes metabolizes drugs to make them more water-soluble for excretion. The proteins 3 beta-hydroxysteroid dehydrogenase and CYP3A4 are some of the related components within these pathways working in conjunction to achieve metabolic homeostasis. P450 reductase assays are often utilized to study these pathways further understanding the biological roles and interactions of these proteins.

CPR has a significant connection to conditions like congenital adrenal hyperplasia and prostate cancer. Mutations or dysregulations in cytochrome P450 reductase can lead to accumulated steroid precursors and disrupted steroid hormone synthesis contributing to these disorders. The relationship with adrenal enzyme 21-hydroxylase and the androgen receptor illustrates the pathway complexities and the clinical relevance of cytochrome P450 reductase. Understanding these connections highlights the importance of CPR in maintaining normal physiological functions and how its abnormalities can lead to serious health issues.
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Specifications

Form

Liquid

Additional notes

Assessed by densitometry. Affinity purified.

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General info

Function

This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.

Sequence similarities

Belongs to the NADPH--cytochrome P450 reductase family.. In the N-terminal section; belongs to the flavodoxin family.. In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.

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Product protocols

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Target data

This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.
See full target information POR
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