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AB134606

Recombinant Human Cytohesin 2 protein

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Recombinant Human Cytohesin 2 protein is a Human Full Length protein, in the 1 to 399 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

ARNO, PSCD2, PSCD2L, CYTH2, Cytohesin-2, ARF exchange factor, ARF nucleotide-binding site opener, Protein ARNO

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SDS-PAGE - Recombinant Human Cytohesin 2 protein (AB134606)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Cytohesin 2 protein (AB134606)

15% SDS-PAGE analysis of ab134606 (3μg).

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Q99418

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMEDGVYEPPDLTPEERMELENIRRRKQELLVEIQRLREELSEAMSEVEGLEANEGSKTLQRNRKMAMGRKKFNMDPKKGIQFLVENELLQNTPEEIARFLYKGEGLNKTAIGDYLGEREELNLAVLHAFVDLHEFTDLNLVQALRQFLWSFRLPGEAQKIDRMMEAFAQRYCLCNPGVFQSTDTCYVLSFAVIMLNTSLHNPNVRDKPGLERFVAMNRGINEGGDLPEELLRNLYDSIRNEPFKIPEDDGNDLTHTFFNPDREGWLLKLGGRVKTWKRRWFILTDNCLYYFEYTTDKEPRGIIPLENLSIREVDDPRKPNCFELYIPNNKGQLIKACKTEADGRVVEGNHMVYRISAPTQEEKDEWIKSIQAAVSVDPFYEMLAARKKRISVKKKQEQP","proteinLength":"Full Length","predictedMolecularWeight":"48.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":399,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q99418","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytohesin 2 also known as ARF nucleotide-binding site opener (ARNO) is a guanine nucleotide exchange factor (GEF) with a molecular mass of approximately 48 kDa. Mechanically Cytohesin 2 activates ADP-ribosylation factors (ARFs) by facilitating the exchange of GDP for GTP which is important for vesicular trafficking and actin cytoskeleton remodeling. This protein is expressed in various tissues with significant presence in the brain lung and immune cells indicating its involvement in multiple cellular processes.
Biological function summary

This protein contributes to cellular signal transduction and membrane dynamics. Cytohesin 2 interacts with the IPCEF1 protein which enhances its ability to act as a GEF for ARF family proteins. As part of the cytohesin protein family it regulates cell adhesion and migration by altering the actin cytoskeleton. It influences integrin-mediated signaling pathways that are critical for immune response and cell communication.

Pathways

Researchers have identified Cytohesin 2 in the PI3K-AKT signaling pathway where it modulates cellular processes such as growth and survival. It interacts with proteins like GRK2 which assists in regulating receptor signaling intensity. Additionally Cytohesin 2 links to Ras-related pathways where it influences small GTPase activities that are important for cell motility and proliferation.

Cytohesin 2 is associated with conditions such as cancer and inflammatory diseases. Altered expression or function of Cytohesin 2 affects cellular migration and adhesion processes often dysregulated in cancer metastasis. In inflammatory diseases Cytohesin 2 interacts with the Rho GTPase family showing its influence on cell migration and immune system regulation. These associations make Cytohesin 2 a potential target for therapeutic research in oncology and immunology.
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Specifications

Form

Liquid

Additional notes

ab134606 was purified using conventional chromatography techniques.

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General info

Function

Acts as a guanine-nucleotide exchange factor (GEF). Promotes guanine-nucleotide exchange on ARF1, ARF3 and ARF6. Activates ARF factors through replacement of GDP with GTP (By similarity). The cell membrane form, in association with ARL4 proteins, recruits ARF6 to the plasma membrane (PubMed : 17398095). Involved in neurite growth (By similarity).

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Product protocols

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Target data

Acts as a guanine-nucleotide exchange factor (GEF). Promotes guanine-nucleotide exchange on ARF1, ARF3 and ARF6. Activates ARF factors through replacement of GDP with GTP (By similarity). The cell membrane form, in association with ARL4 proteins, recruits ARF6 to the plasma membrane (PubMed : 17398095). Involved in neurite growth (By similarity).
See full target information CYTH2
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