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AB156970

Recombinant Human Cytokeratin 8 protein (His tag N-Terminus)

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(2 Publications)

Recombinant Human Cytokeratin 8 protein is a Human Full Length protein, in the 1 to 483 aa range with >90% purity and suitable for SDS-PAGE.The predicted molecular weight of ab156970 protein is 56.1 kDa.

- Save time and ensure accurate results - use our recombinant human Cytokeratin 8 (KRT8) as a control

View Alternative Names

CYK8, KRT8, Cytokeratin-8, Keratin-8, Type-II keratin Kb8, CK-8, K8

1 Images
SDS-PAGE - Recombinant Human Cytokeratin 8 protein (His tag N-Terminus) (AB156970)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Cytokeratin 8 protein (His tag N-Terminus) (AB156970)

15% SDS-PAGE analysis of ab156970 (3 µg).

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P05787

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Ensure the validity of your result using our recombinant human Cytokeratin 8 (KRT8) ab156970 as positive control in SDS-PAGE.


Check out our protein gel staining guide for SDS-PAGE here
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMSIRVTQKSYKVSTSGPRAFSSRSYTSGPGSRISSSSFSRVGSSNFRGGLGGGYGGASGMGGITAVTVNQSLLSPLVLEVDPNIQAVRTQEKEQIKTLNNKFASFIDKVRFLEQQNKMLETKWSLLQQQKTARSNMDNMFESYINNLRRQLETLGQEKLKLEAELGNMQGLVEDFKNKYEDEINKRTEMENEFVLIKKDVDEAYMNKVELESRLEGLTDEINFLRQLYEEEIRELQSQISDTSVVLSMDNSRSLDMDSIIAEVKAQYEDIANRSRAEAESMYQIKYEELQSLAGKHGDDLRRTKTEISEMNRNISRLQAEIEGLKGQRASLEAAIADAEQRGELAIKDANAKLSELEAALQRAKQDMARQLREYQELMNVKLALDIEIATYRKLLEGEESRLESGMQNMSIHTKTTSGYAGGLSSAYGGLTSPGLSYSLGSSFGSGAGSSSFSRTSSSRAVVVKKIETRDGKLVSESSDVLPK","proteinLength":"Full Length","predictedMolecularWeight":"56.14 kDa","actualMolecularWeight":null,"aminoAcidEnd":483,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P05787","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

Additional notes

ab156970 is purified using conventional chromatography techniques.

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General info

Function

Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

Sequence similarities

Belongs to the intermediate filament family.

Post-translational modifications

Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.. O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.. O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
See full target information KRT8
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Tissue barriers 11:2039003 PubMed35262466

2022

GRP75 as a functional element of cholix transcytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Keyi Liu,Tom Hunter,Alistair Taverner,Kevin Yin,Julia MacKay,Kate Colebrook,Morgan Correia,Amandine Rapp,Randall J Mrsny

Frontiers in molecular neuroscience 10:259 PubMed28878619

2017

miRNA Enriched in Human Neuroblast Nuclei Bind the MAZ Transcription Factor and Their Precursors Contain the MAZ Consensus Motif.

Applications

Unspecified application

Species

Unspecified reactive species

Belinda J Goldie,Chantel Fitzsimmons,Judith Weidenhofer,Joshua R Atkins,Dan O Wang,Murray J Cairns
View all publications
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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