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Recombinant human DAGK protein (Active) is a Human Full Length protein, in the 1 to 942 aa range, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, FuncS.

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Images

Functional Studies - Recombinant human DAGK protein (Active) (AB268444), expandable thumbnail
  • SDS-PAGE - Recombinant human DAGK protein (Active) (AB268444), expandable thumbnail

Key facts

Purity
>90% SDS-PAGE
Expression system
Baculovirus infected Sf9 cells
Tags
GST tag N-Terminus
Applications
SDS-PAGE, FuncS
Biologically active
Yes

Amino acid sequence

M A A A A E P G A R A W L G G G S P R P G S P A C S P V L G S G G R A R P G P G P G P G P E R A G V R A P G P A A A P G H S F R K V T L T K P T F C H L C S D F I W G L A G F L C D V C N F M S H E K C L K H V R I P C T S V A P S L V R V P V A H C F G P R G L H K R K F C A V C R K V L E A P A L H C E V C E L H L H P D C V P F A C S D C R Q C H Q D G H Q D H D T H H H H W R E G N L P S G A R C E V C R K T C G S S D V L A G V R C E W C G V Q A H S L C S A A L A P E C G F G R L R S L V L P P A C V R L L P G G F S K T Q S F R I V E A A E P G E G G D G A D G S A A V G P G R E T Q A T P E S G K Q T L K I F D G D D A V R R S Q F R L V T V S R L A G A E E V L E A A L R A H H I P E D P G H L E L C R L P P S S Q A C D A W A G G K A G S A V I S E E G R S P G S G E A T P E A W V I R A L P R A Q E V L K I Y P G W L K V G V A Y V S V R V T P K S T A R S V V L E V L P L L G R Q A E S P E S F Q L V E V A M G C R H V Q R T M L M D E Q P L L D R L Q D I R Q M S V R Q V S Q T R F Y V A E S R D V A P H V S L F V G G L P P G L S P E E Y S S L L H E A G A T K A T V V S V S H I Y S S Q G A V V L D V A C F A E A E R L Y M L L K D M A V R G R L L T A L V L P D L L H A K L P P D S C P L L V F V N P K S G G L K G R D L L C S F R K L L N P H Q V F D L T N G G P L P G L H L F S Q V P C F R V L V C G G D G T V G W V L G A L E E T R Y R L A C P E P S V A I L P L G T G N D L G R V L R W G A G Y S G E D P F S V L L S V D E A D A V L M D R W T I L L D A H E A G S A E N D T A D A E P P K I V Q M S N Y C G I G I D A E L S L D F H Q A R E E E P G K F T S R L H N K G V Y V R V G L Q K I S H S R S L H K Q I R L Q V E R Q E V E L P S I E G L I F I N I P S W G S G A D L W G S D S D T R F E K P R M D D G L L E V V G V T G V V H M G Q V Q G G L R S G I R I A Q G S Y F R V T L L K A T P V Q V D G E P W V Q A P G H M I I S A A G P K V H M L R K A K Q K P R R A G T T R D A R A D A A P A P E S D P R

Reactivity data

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Application
FuncS
Reactivity
Reacts
Dilution info
-
Notes

-

Target data

Function

Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed:11309392, PubMed:22627129, PubMed:9099683). Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (PubMed:11309392, PubMed:17664281, PubMed:26748701). Within the adrenocorticotropic hormone signaling pathway, produces phosphatidic acid which in turn activates NR5A1 and subsequent steroidogenic gene transcription (PubMed:17664281). Also functions downstream of the nerve growth factor signaling pathway being specifically activated in the nucleus by the growth factor (By similarity). Through its diacylglycerol activity also regulates synaptic vesicle endocytosis (PubMed:26748701).

Alternative names

Recommended products

Recombinant human DAGK protein (Active) is a Human Full Length protein, in the 1 to 942 aa range, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, FuncS.

Key facts

Purity
>90% SDS-PAGE
Expression system
Baculovirus infected Sf9 cells
Applications
SDS-PAGE, FuncS
Biological activity
The specific activity of ab268444 was 27.5 nmol/min/mg in a kinase assay using a Dilauroyl-sn-glycerol as substrate.
Accession
P52824-1
Animal free
No
Species
Human
Concentration
Loading...
Storage buffer

pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

Sequence info

Amino acid sequence

M A A A A E P G A R A W L G G G S P R P G S P A C S P V L G S G G R A R P G P G P G P G P E R A G V R A P G P A A A P G H S F R K V T L T K P T F C H L C S D F I W G L A G F L C D V C N F M S H E K C L K H V R I P C T S V A P S L V R V P V A H C F G P R G L H K R K F C A V C R K V L E A P A L H C E V C E L H L H P D C V P F A C S D C R Q C H Q D G H Q D H D T H H H H W R E G N L P S G A R C E V C R K T C G S S D V L A G V R C E W C G V Q A H S L C S A A L A P E C G F G R L R S L V L P P A C V R L L P G G F S K T Q S F R I V E A A E P G E G G D G A D G S A A V G P G R E T Q A T P E S G K Q T L K I F D G D D A V R R S Q F R L V T V S R L A G A E E V L E A A L R A H H I P E D P G H L E L C R L P P S S Q A C D A W A G G K A G S A V I S E E G R S P G S G E A T P E A W V I R A L P R A Q E V L K I Y P G W L K V G V A Y V S V R V T P K S T A R S V V L E V L P L L G R Q A E S P E S F Q L V E V A M G C R H V Q R T M L M D E Q P L L D R L Q D I R Q M S V R Q V S Q T R F Y V A E S R D V A P H V S L F V G G L P P G L S P E E Y S S L L H E A G A T K A T V V S V S H I Y S S Q G A V V L D V A C F A E A E R L Y M L L K D M A V R G R L L T A L V L P D L L H A K L P P D S C P L L V F V N P K S G G L K G R D L L C S F R K L L N P H Q V F D L T N G G P L P G L H L F S Q V P C F R V L V C G G D G T V G W V L G A L E E T R Y R L A C P E P S V A I L P L G T G N D L G R V L R W G A G Y S G E D P F S V L L S V D E A D A V L M D R W T I L L D A H E A G S A E N D T A D A E P P K I V Q M S N Y C G I G I D A E L S L D F H Q A R E E E P G K F T S R L H N K G V Y V R V G L Q K I S H S R S L H K Q I R L Q V E R Q E V E L P S I E G L I F I N I P S W G S G A D L W G S D S D T R F E K P R M D D G L L E V V G V T G V V H M G Q V Q G G L R S G I R I A Q G S Y F R V T L L K A T P V Q V D G E P W V Q A P G H M I I S A A G P K V H M L R K A K Q K P R R A G T T R D A R A D A A P A P E S D P R
Accession
P52824
Protein length
Full Length
Amino acids
1 to 942
Nature
Recombinant
Tags
GST tag N-Terminus

Specifications

Form
Liquid
Additional notes

Affinity purified.

General info

Function

Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed:11309392, PubMed:22627129, PubMed:9099683). Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (PubMed:11309392, PubMed:17664281, PubMed:26748701). Within the adrenocorticotropic hormone signaling pathway, produces phosphatidic acid which in turn activates NR5A1 and subsequent steroidogenic gene transcription (PubMed:17664281). Also functions downstream of the nerve growth factor signaling pathway being specifically activated in the nucleus by the growth factor (By similarity). Through its diacylglycerol activity also regulates synaptic vesicle endocytosis (PubMed:26748701).

Sequence similarities

Belongs to the eukaryotic diacylglycerol kinase family.

Post-translational modifications

Phosphorylated by PRKCE and PRKCH in vitro.

Subcellular localisation
Cytoskeleton, Nucleus, Nucleus speckle, Nucleus matrix

Storage

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

This product is an active protein and may elicit a biological response in vivo, handle with caution.

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Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Functional Studies - Recombinant human DAGK protein (Active) (ab268444), expandable thumbnail

    Functional Studies - Recombinant human DAGK protein (Active) (ab268444)

    The specific activity of ab268444 was 27.5 nmol/min/mg in a kinase assay using a Dilauroyl-sn-glycerol as substrate.

  • SDS-PAGE - Recombinant human DAGK protein (Active) (ab268444), expandable thumbnail

    SDS-PAGE - Recombinant human DAGK protein (Active) (ab268444)

    SDS-PAGE analysis of ab268444.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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