Recombinant human DAGK protein (Active) is a Human Full Length protein, in the 1 to 942 aa range, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, FuncS.
M A A A A E P G A R A W L G G G S P R P G S P A C S P V L G S G G R A R P G P G P G P G P E R A G V R A P G P A A A P G H S F R K V T L T K P T F C H L C S D F I W G L A G F L C D V C N F M S H E K C L K H V R I P C T S V A P S L V R V P V A H C F G P R G L H K R K F C A V C R K V L E A P A L H C E V C E L H L H P D C V P F A C S D C R Q C H Q D G H Q D H D T H H H H W R E G N L P S G A R C E V C R K T C G S S D V L A G V R C E W C G V Q A H S L C S A A L A P E C G F G R L R S L V L P P A C V R L L P G G F S K T Q S F R I V E A A E P G E G G D G A D G S A A V G P G R E T Q A T P E S G K Q T L K I F D G D D A V R R S Q F R L V T V S R L A G A E E V L E A A L R A H H I P E D P G H L E L C R L P P S S Q A C D A W A G G K A G S A V I S E E G R S P G S G E A T P E A W V I R A L P R A Q E V L K I Y P G W L K V G V A Y V S V R V T P K S T A R S V V L E V L P L L G R Q A E S P E S F Q L V E V A M G C R H V Q R T M L M D E Q P L L D R L Q D I R Q M S V R Q V S Q T R F Y V A E S R D V A P H V S L F V G G L P P G L S P E E Y S S L L H E A G A T K A T V V S V S H I Y S S Q G A V V L D V A C F A E A E R L Y M L L K D M A V R G R L L T A L V L P D L L H A K L P P D S C P L L V F V N P K S G G L K G R D L L C S F R K L L N P H Q V F D L T N G G P L P G L H L F S Q V P C F R V L V C G G D G T V G W V L G A L E E T R Y R L A C P E P S V A I L P L G T G N D L G R V L R W G A G Y S G E D P F S V L L S V D E A D A V L M D R W T I L L D A H E A G S A E N D T A D A E P P K I V Q M S N Y C G I G I D A E L S L D F H Q A R E E E P G K F T S R L H N K G V Y V R V G L Q K I S H S R S L H K Q I R L Q V E R Q E V E L P S I E G L I F I N I P S W G S G A D L W G S D S D T R F E K P R M D D G L L E V V G V T G V V H M G Q V Q G G L R S G I R I A Q G S Y F R V T L L K A T P V Q V D G E P W V Q A P G H M I I S A A G P K V H M L R K A K Q K P R R A G T T R D A R A D A A P A P E S D P R
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed:11309392, PubMed:22627129, PubMed:9099683). Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (PubMed:11309392, PubMed:17664281, PubMed:26748701). Within the adrenocorticotropic hormone signaling pathway, produces phosphatidic acid which in turn activates NR5A1 and subsequent steroidogenic gene transcription (PubMed:17664281). Also functions downstream of the nerve growth factor signaling pathway being specifically activated in the nucleus by the growth factor (By similarity). Through its diacylglycerol activity also regulates synaptic vesicle endocytosis (PubMed:26748701).
DAGK4, DGKQ, Diacylglycerol kinase theta, DAG kinase theta, DGKtheta, Diglyceride kinase theta, DGK-theta
Recombinant human DAGK protein (Active) is a Human Full Length protein, in the 1 to 942 aa range, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, FuncS.
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF
Affinity purified.
Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed:11309392, PubMed:22627129, PubMed:9099683). Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (PubMed:11309392, PubMed:17664281, PubMed:26748701). Within the adrenocorticotropic hormone signaling pathway, produces phosphatidic acid which in turn activates NR5A1 and subsequent steroidogenic gene transcription (PubMed:17664281). Also functions downstream of the nerve growth factor signaling pathway being specifically activated in the nucleus by the growth factor (By similarity). Through its diacylglycerol activity also regulates synaptic vesicle endocytosis (PubMed:26748701).
Belongs to the eukaryotic diacylglycerol kinase family.
Phosphorylated by PRKCE and PRKCH in vitro.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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The specific activity of ab268444 was 27.5 nmol/min/mg in a kinase assay using a Dilauroyl-sn-glycerol as substrate.
SDS-PAGE analysis of ab268444.
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