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AB112279

Recombinant Human DAZL protein (GST tag N-Terminus)

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Recombinant Human DAZL protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 296 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, PepArr, WB.

View Alternative Names

DAZH, DAZL1, DAZLA, SPGYLA, DAZL, Deleted in azoospermia-like, DAZ homolog, DAZ-like autosomal, Deleted in azoospermia-like 1, SPGY-like-autosomal

2 Images
Western blot - Recombinant Human DAZL protein (GST tag N-Terminus) (AB112279)
  • WB

Lab

Western blot - Recombinant Human DAZL protein (GST tag N-Terminus) (AB112279)

All lanes:

Western blot - Anti-DAZL antibody (<a href='/en-us/products/primary-antibodies/dazl-antibody-ab34139'>ab34139</a>) at 1 µg/mL

Lane 1:

GST tagged Recombinant Human DAZL protein (ab112279) at 0.1 µg

Lane 1:

Western blot - Recombinant Human DAZL protein (GST tag N-Terminus) (ab112279) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 33 kDa

true

Exposure time: 10s

SDS-PAGE - Recombinant Human DAZL protein (GST tag N-Terminus) (AB112279)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human DAZL protein (GST tag N-Terminus) (AB112279)

12.5% SDS-PAGE Stained with Coomassie Blue with recombinant protein.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

SDS-PAGE, WB, PepArr, ELISA

applications

Biologically active

No

Accession

Q92904

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "PepArr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.ab112279 can be used as a WB positive control in conjunction with <a href='/en-us/products/primary-antibodies/dazl-antibody-ab34139'>ab34139</a>.</p>" } } }

Product details

This product is useful for Antibody Production and Protein Array.

Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"58.19 kDa","actualMolecularWeight":null,"aminoAcidEnd":296,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q92904","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]

Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False

Specifications

Form

Liquid

General info

Function

RNA-binding protein, which is essential for gametogenesis in both males and females. Plays a central role during spermatogenesis. Acts by binding to the 3'-UTR of mRNA, specifically recognizing GUU triplets, and thereby regulating the translation of key transcripts (By similarity).

Sequence similarities

Belongs to the RRM DAZ family.

Subcellular localisation

Nucleus

Product protocols

Target data

RNA-binding protein, which is essential for gametogenesis in both males and females. Plays a central role during spermatogenesis. Acts by binding to the 3'-UTR of mRNA, specifically recognizing GUU triplets, and thereby regulating the translation of key transcripts (By similarity).
See full target information DAZL

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