Recombinant Human DC-SIGN protein (Fc Chimera) is a Human Fragment protein, in the 62 to 404 aa range, expressed in HEK 293, with >97% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
>97% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
HEK 293 cells
- Tags
Fc tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
Amino acid sequence
K V P S S I S Q E Q S R Q D A I Y Q N L T Q L K A A V G E L S E K S K L Q E I Y Q E L T Q L K A A V G E L P E K S K L Q E I Y Q E L T R L K A A V G E L P E K S K L Q E I Y Q E L T W L K A A V G E L P E K S K M Q E I Y Q E L T R L K A A V G E L P E K S K Q Q E I Y Q E L T R L K A A V G E L P E K S K Q Q E I Y Q E L T R L K A A V G E L P E K S K Q Q E I Y Q E L T Q L K A A V E R L C H P C P W E W T F F Q G N C Y F M S N S Q R N W H D S I T A C K E V G A Q L V V I K S A E E Q N F L Q L Q S S R S N R F T W M G L S D L N Q E G T W Q W V D G S P L L P S F K Q Y W N R G E P N N V G E E D C A E F S G N G W N D D K C N L A K F W I C K K S A A S C S R D E E Q F L S P A P A T P N P P P A
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Pathogen-recognition receptor expressed on the surface of immature dendritic cells (DCs) and involved in initiation of primary immune response. Thought to mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. The receptor returns to the cell membrane surface and the pathogen-derived antigens are presented to resting T-cells via MHC class II proteins to initiate the adaptive immune response.On DCs it is a high affinity receptor for ICAM2 and ICAM3 by binding to mannose-like carbohydrates. May act as a DC rolling receptor that mediates transendothelial migration of DC presursors from blood to tissues by binding endothelial ICAM2. Seems to regulate DC-induced T-cell proliferation by binding to ICAM3 on T-cells in the immunological synapse formed between DC and T-cells.(Microbial infection) Acts as an attachment receptor for HIV-1 and HIV-2.(Microbial infection) Acts as an attachment receptor for Ebolavirus.(Microbial infection) Acts as an attachment receptor for Cytomegalovirus.(Microbial infection) Acts as an attachment receptor for HCV.(Microbial infection) Acts as an attachment receptor for Dengue virus.(Microbial infection) Acts as an attachment receptor for Measles virus.(Microbial infection) Acts as an attachment receptor for Herpes simplex virus 1.(Microbial infection) Acts as an attachment receptor for Influenzavirus A.(Microbial infection) Acts as an attachment receptor for SARS-CoV.(Microbial infection) Acts as an attachment receptor for Japanese encephalitis virus.(Microbial infection) Acts as an attachment receptor for Lassa virus (PubMed:23966408). Acts as an attachment receptor for Marburg virusn.(Microbial infection) Acts as an attachment receptor for Respiratory syncytial virus.(Microbial infection) Acts as an attachment receptor for Rift valley fever virus and uukuniemi virus.(Microbial infection) Acts as an attachment receptor for West-nile virus.(Microbial infection) Probably recognizes in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of bacterial pathogen antigens, including Leishmania pifanoi LPG, Lewis-x antigen in Helicobacter pylori LPS, mannose in Klebsiella pneumonae LPS, di-mannose and tri-mannose in Mycobacterium tuberculosis ManLAM and Lewis-x antigen in Schistosoma mansoni SEA (PubMed:16379498). Recognition of M.tuberculosis by dendritic cells occurs partially via this molecule (PubMed:16092920, PubMed:21203928).
Alternative names
CD209 antigen, C-type lectin domain family 4 member L, Dendritic cell-specific ICAM-3-grabbing non-integrin 1, DC-SIGN, DC-SIGN1, CD209, CLEC4L
Recommended products
Recombinant Human DC-SIGN protein (Fc Chimera) is a Human Fragment protein, in the 62 to 404 aa range, expressed in HEK 293, with >97% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Alternative names
CD209 antigen, C-type lectin domain family 4 member L, Dendritic cell-specific ICAM-3-grabbing non-integrin 1, DC-SIGN, DC-SIGN1, CD209, CLEC4L
Key facts
- Purity
>97% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
HEK 293 cells
- Tags
Fc tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
- Accession
- Q9NNX6-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 100% PBS
Sequence info
Amino acid sequence
K V P S S I S Q E Q S R Q D A I Y Q N L T Q L K A A V G E L S E K S K L Q E I Y Q E L T Q L K A A V G E L P E K S K L Q E I Y Q E L T R L K A A V G E L P E K S K L Q E I Y Q E L T W L K A A V G E L P E K S K M Q E I Y Q E L T R L K A A V G E L P E K S K Q Q E I Y Q E L T R L K A A V G E L P E K S K Q Q E I Y Q E L T R L K A A V G E L P E K S K Q Q E I Y Q E L T Q L K A A V E R L C H P C P W E W T F F Q G N C Y F M S N S Q R N W H D S I T A C K E V G A Q L V V I K S A E E Q N F L Q L Q S S R S N R F T W M G L S D L N Q E G T W Q W V D G S P L L P S F K Q Y W N R G E P N N V G E E D C A E F S G N G W N D D K C N L A K F W I C K K S A A S C S R D E E Q F L S P A P A T P N P P P A
- Accession
- Protein length
Fragment
- Predicted molecular weight
65.8 kDa
- Amino acids
62 to 404
- Nature
Recombinant
- Tags
Fc tag N-Terminus
Specifications
- Form
Lyophilized
General info
- Function
Pathogen-recognition receptor expressed on the surface of immature dendritic cells (DCs) and involved in initiation of primary immune response. Thought to mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. The receptor returns to the cell membrane surface and the pathogen-derived antigens are presented to resting T-cells via MHC class II proteins to initiate the adaptive immune response.
Storage
- Shipped at conditions
Ambient - Can Ship with Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Product promise
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1 product image
SDS-PAGE - Recombinant Human DC-SIGN protein (Fc Chimera) (ab276226)
SDS-PAGE analysis of ab276226
Downloads
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