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AB132368

Recombinant Human Dcp2/TDT protein

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Recombinant Human Dcp2/TDT protein is a Human Full Length protein, in the 1 to 385 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

NUDT20, DCP2, m7GpppN-mRNA hydrolase, Nucleoside diphosphate-linked moiety X motif 20, mRNA-decapping enzyme 2, Nudix motif 20, hDpc

1 Images
SDS-PAGE - Recombinant Human Dcp2/TDT protein (AB132368)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Dcp2/TDT protein (AB132368)

12.5% SDS-PAGE analysis of ab132368 stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

Tag free

Applications

ELISA, SDS-PAGE, WB

applications

Biologically active

No

Accession

Q8IU60

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as Dcp2.
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Sequence info

[{"sequence":"METKRVEIPGSVLDDLCSRFILHIPSEERDNAIRVCFQIELAHWFYLDFYMQNTPGLPQCGIRDFAKAVFSHCPFLLPQGEDVEKVLDEWKEYKMGVPTYGAIILDETLENVLLVQGYLAKSGWGFPKGKVNKEEAPHDCAAREVFEETGFDIKDYICKDDYIELRINDQLARLYIIPGIPKDTKFNPKTRREIRNIEWFSIEKLPCHRNDMTPKSKLGLAPNKFFMAIPFIRPLRDWLSRRFGDSSDSDNGFSSTGSTPAKPTVEKLSRTKFRHSQQLFPDGSPGDQWVKHRQPLQQKPYNNHSEMSDLLKGKKCEKKLHPRKLQDNFETDAVYDLPSSSEDQLLEHAEGQPVACNGHCKFPFSSRAFLSFKFDHNAIMKILDL","proteinLength":"Full Length","predictedMolecularWeight":"68.09 kDa","actualMolecularWeight":null,"aminoAcidEnd":385,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q8IU60","tags":[]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

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General info

Function

Decapping metalloenzyme that catalyzes the cleavage of the cap structure on mRNAs (PubMed : 12218187, PubMed : 12417715, PubMed : 12923261, PubMed : 21070968, PubMed : 28002401, PubMed : 31875550). Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP (PubMed : 12486012, PubMed : 12923261, PubMed : 21070968, PubMed : 28002401, PubMed : 31875550). Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay (PubMed : 14527413). Plays a role in replication-dependent histone mRNA degradation (PubMed : 18172165). Has higher activity towards mRNAs that lack a poly(A) tail (PubMed : 21070968). Has no activity towards a cap structure lacking an RNA moiety (PubMed : 21070968). The presence of a N(6)-methyladenosine methylation at the second transcribed position of mRNAs (N(6),2'-O-dimethyladenosine cap; m6A(m)) provides resistance to DCP2-mediated decapping (PubMed : 28002401). Blocks autophagy in nutrient-rich conditions by repressing the expression of ATG-related genes through degradation of their transcripts (PubMed : 26098573).

Sequence similarities

Belongs to the Nudix hydrolase family. DCP2 subfamily.

Post-translational modifications

Phosphorylated at ser-249 in a MTOR-dependent manner (PubMed:26098573).

Subcellular localisation

Nucleus

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Product protocols

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Target data

Decapping metalloenzyme that catalyzes the cleavage of the cap structure on mRNAs (PubMed : 12218187, PubMed : 12417715, PubMed : 12923261, PubMed : 21070968, PubMed : 28002401, PubMed : 31875550). Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP (PubMed : 12486012, PubMed : 12923261, PubMed : 21070968, PubMed : 28002401, PubMed : 31875550). Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay (PubMed : 14527413). Plays a role in replication-dependent histone mRNA degradation (PubMed : 18172165). Has higher activity towards mRNAs that lack a poly(A) tail (PubMed : 21070968). Has no activity towards a cap structure lacking an RNA moiety (PubMed : 21070968). The presence of a N(6)-methyladenosine methylation at the second transcribed position of mRNAs (N(6),2'-O-dimethyladenosine cap; m6A(m)) provides resistance to DCP2-mediated decapping (PubMed : 28002401). Blocks autophagy in nutrient-rich conditions by repressing the expression of ATG-related genes through degradation of their transcripts (PubMed : 26098573).
See full target information DCP2
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