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AB103056

Recombinant Human DCUN1D1 protein

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Recombinant Human DCUN1D1 protein is a Human Full Length protein, in the 1 to 259 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

DCN1, DCUN1L1, RP42, SCCRO, DCUN1D1, DCN1-like protein 1, DCNL1, DCUN1 domain-containing protein 1, Defective in cullin neddylation protein 1-like protein 1, Squamous cell carcinoma-related oncogene

1 Images
SDS-PAGE - Recombinant Human DCUN1D1 protein (AB103056)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human DCUN1D1 protein (AB103056)

15% SDS-PAGE (3 μg)

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

Q96GG9

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMNKLKSSQKDKVRQFMIFTQSSEKTAVSCLSQNDWKLDVATDNFFQNPELYIRESVKGSLDRKKLEQLYNRYKDPQDENKIGIDGIQQFCDDLALDPASISVLIIAWKFRAATQCEFSKQEFMDGMTELGCDSIEKLKAQIPKMEQELKEPGRFKDFYQFTFNFAKNPGQKGLDLEMAIAYWNLVLNGRFKFLDLWNKFLLEHHKRSIPKDTWNLLLDFSTMIADDMSNYDEEGAWPVLIDDFVEFARPQIAGTKSTTV","proteinLength":"Full Length","predictedMolecularWeight":"32.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":259,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q96GG9","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

Additional notes

ab103056 was purified using conventional chromatography.

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General info

Function

Part of an E3 ubiquitin ligase complex for neddylation (PubMed : 18826954). Promotes neddylation of cullin components of E3 cullin-RING ubiquitin ligase complexes (PubMed : 19617556, PubMed : 23201271, PubMed : 23401859, PubMed : 26906416). Acts by binding to cullin-RBX1 complexes in the cytoplasm and promoting their nuclear translocation, enhancing recruitment of E2-NEDD8 (UBE2M-NEDD8) thioester to the complex, and optimizing the orientation of proteins in the complex to allow efficient transfer of NEDD8 from the E2 to the cullin substrates. Involved in the release of inhibitory effets of CAND1 on cullin-RING ligase E3 complex assembly and activity (PubMed : 25349211, PubMed : 28581483). Acts also as an oncogene facilitating malignant transformation and carcinogenic progression (By similarity).

Post-translational modifications

Mono- and poly-ubiquitinated by ARIH2 and ARIH1. Monoubiquitination by ARIH2 is mediated by an interaction between autoubiquitinated ARIH2 and the UBA-like domain. The monoubiquitinated form preferentially interacts with non-neddylated cullins and modulates cullin RING ligase (CRL) complex composition and activity.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Part of an E3 ubiquitin ligase complex for neddylation (PubMed : 18826954). Promotes neddylation of cullin components of E3 cullin-RING ubiquitin ligase complexes (PubMed : 19617556, PubMed : 23201271, PubMed : 23401859, PubMed : 26906416). Acts by binding to cullin-RBX1 complexes in the cytoplasm and promoting their nuclear translocation, enhancing recruitment of E2-NEDD8 (UBE2M-NEDD8) thioester to the complex, and optimizing the orientation of proteins in the complex to allow efficient transfer of NEDD8 from the E2 to the cullin substrates. Involved in the release of inhibitory effets of CAND1 on cullin-RING ligase E3 complex assembly and activity (PubMed : 25349211, PubMed : 28581483). Acts also as an oncogene facilitating malignant transformation and carcinogenic progression (By similarity).
See full target information DCUN1D1
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