Recombinant Human DDI2 protein is a Human Full Length protein, in the 1 to 211 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Images
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
M L L T V Y C V R R D L S E V T F S L Q V D A D F E L H N F R A L C E L E S G I P A A E S Q I V Y A E R P L T D N H R S L A S Y G L K D G D V V I L R Q K E N A D P R P P V Q F P N L P R I D F S S I A V P G T S S P R Q R Q P P G T Q Q S H S S P G E I T S S P Q G L D N P A L L R D M L L A N P H E L S L L K E R N P P L A E A L L S G D L E K F S R V L V E Q Q Q D R A R R E Q E R I R L F S A D P F D L E A Q A K I E E D I R
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Aspartic protease that mediates the cleavage of NFE2L1/NRF1 at 'Leu-104', thereby promoting release of NFE2L1/NRF1 from the endoplasmic reticulum membrane (PubMed:27528193, PubMed:27676298). Ubiquitination of NFE2L1/NRF1 is a prerequisite for cleavage, suggesting that DDI2 specifically recognizes and binds ubiquitinated NFE2L1/NRF1 (PubMed:27528193). Seems to act as a proteasomal shuttle which links the proteasome and replication fork proteins like RTF2 (Probable). Required, with DDI1, for cellular survival following replication stress. Together or redudantly with DDI1, removes RTF2 from stalled forks to allow cell cycle progression after replication stress and maintains genome integrity (PubMed:29290612).
Alternative names
Protein DDI1 homolog 2, DDI2
Recommended products
Recombinant Human DDI2 protein is a Human Full Length protein, in the 1 to 211 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- Q5TDH0-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M L L T V Y C V R R D L S E V T F S L Q V D A D F E L H N F R A L C E L E S G I P A A E S Q I V Y A E R P L T D N H R S L A S Y G L K D G D V V I L R Q K E N A D P R P P V Q F P N L P R I D F S S I A V P G T S S P R Q R Q P P G T Q Q S H S S P G E I T S S P Q G L D N P A L L R D M L L A N P H E L S L L K E R N P P L A E A L L S G D L E K F S R V L V E Q Q Q D R A R R E Q E R I R L F S A D P F D L E A Q A K I E E D I R
- Accession
- Q5TDH0
- Protein length
- Full Length
- Amino acids
- 1 to 211
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Aspartic protease that mediates the cleavage of NFE2L1/NRF1 at 'Leu-104', thereby promoting release of NFE2L1/NRF1 from the endoplasmic reticulum membrane (PubMed:27528193, PubMed:27676298). Ubiquitination of NFE2L1/NRF1 is a prerequisite for cleavage, suggesting that DDI2 specifically recognizes and binds ubiquitinated NFE2L1/NRF1 (PubMed:27528193). Seems to act as a proteasomal shuttle which links the proteasome and replication fork proteins like RTF2 (Probable). Required, with DDI1, for cellular survival following replication stress. Together or redudantly with DDI1, removes RTF2 from stalled forks to allow cell cycle progression after replication stress and maintains genome integrity (PubMed:29290612).
- Sequence similarities
Belongs to the DDI1 family.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
DDI2 also known as DNA-damage inducible 1 homolog 2 is a protein involved in several important cellular processes. It functions as an aspartic protease known for its capability to cleave ubiquitinated substrates. This protein has a molecular mass of approximately 50 kDa. Researchers often find DDI2 expression in various human tissues indicating its potential roles in diverse biological processes.
- Biological function summary
DDI2 participates in the proteasome-mediated pathway a significant cellular mechanism that regulates protein degradation to maintain cellular homeostasis. It seems to play a part in the endoplasmic reticulum-associated degradation (ERAD) pathway where misfolded proteins get recognized and directed towards destruction. DDI2's involvement in these pathways suggests its critical function in protein quality control. It also may interact with other proteins that contribute to its protease activity enhancing its regulatory role within the cell.
- Pathways
DDI2 functions in the context of the ubiquitin-proteasome system (UPS) a fundamental cellular pathway responsible for protein turnover. Through its protease activity DDI2 intersects with pathways involved in the degradation of damaged or misfolded proteins which connects it to the larger ERAD pathway. Evidence shows its interaction with proteins like p97/VCP which assists in protein degradation and ubiquitin which tags proteins for proteasomal activity highlighting DDI2's place in cellular quality control.
- Associated diseases and disorders
DDI2 is associated with several conditions involving protein homeostasis deficits. Its involvement in lipid metabolism disorders points to a potential role in conditions like Niemann-Pick disease. DDI2 dysfunction may result in the inadequate breakdown of proteins contributing to the pathological accumulation found in disorders related to lipid metabolism. Its interaction with the protein p97/VCP known to be involved in various degenerative diseases highlights the importance of understanding DDI2's mechanistic role in these pathologies.
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1 product image
SDS-PAGE - Recombinant Human DDI2 protein (ab164562)
ab164562 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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