JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB132709

Recombinant Human DDOST protein (GST tag N-Terminus)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human DDOST protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 456 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

KIAA0115, OST48, OK/SW-cl.45, DDOST, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit, DDOST 48 kDa subunit, Oligosaccharyl transferase 48 kDa subunit

1 Images
SDS-PAGE - Recombinant Human DDOST protein (GST tag N-Terminus) (AB132709)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human DDOST protein (GST tag N-Terminus) (AB132709)

ab132709 analyzed by 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, ELISA, SDS-PAGE

applications

Biologically active

No

Accession

P39656

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

style
left-column

Complementary Products

Primary Antibodies

AB204314

Anti-DDOST antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDOST antibody (AB204314)

  • 0
  • 0
Reagents, Controls & Accessories

AB104139

Mounting Medium With DAPI - Aqueous, Fluoroshield

Immunohistochemistry (Frozen sections) - Mounting Medium With DAPI - Aqueous, Fluoroshield (AB104139)

  • 5
  • 9
Primary Antibodies

AB302556

Anti-KPNA4 antibody [EPR26347-21]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPNA4 antibody [EPR26347-21] (AB302556)

  • 0
  • 0
Proteins & Peptides

AB101664

Recombinant Human ERP27 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human ERP27 protein (His tag N-Terminus) (AB101664)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"MGYFRCAGAGSFGRRRKMEPSTAARAWALFWLLLPLLGAVCASGPRTLVLLDNLNVRETHSLFFRSLKDRGFELTFKTADDPSLSLIKYGEFLYDNLIIFSPSVEDFGGNINVETISAFIDGGGSVLVAASSDIGDPLRELGSECGIEFDEEKTAVIDHHNYDISDLGQHTLIVADTENLLKAPTIVGKSSLNPILFRGVGMVADPDNPLVLDILTGSSTSYSFFPDKPITQYPHAVGKNTLLIAGLQARNNARVIFSGSLDFFSDSFFNSAVQKAAPGSQRYSQTGNYELAVALSRWVFKEEGVLRVGPVSHHRVGETAPPNAYTVTDLVEYSIVIQQLSNGKWVPFDGDDIQLEFVRIDPFVRTFLKKKGGKYSVQFKLPDVYGVFQFKVDYNRLGYTHLYSSTQVSVRPLQHTQYERFIPSAYPYYASAFSMMLGLFIFSIVFLHMKEKEKSD","proteinLength":"Full Length","predictedMolecularWeight":"77.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":456,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P39656","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit (DDOST) also known as oligosaccharyltransferase 48 kDa subunit weighs approximately 48 kDa. It is part of the oligosaccharyltransferase (OST) complex which is found mostly in the endoplasmic reticulum (ER). This protein functions to transfer glycan chains to nascent proteins during N-linked glycosylation an important post-translational modification. DDOST expresses widely across different tissues reflecting its importance in protein synthesis and folding.
Biological function summary

The DDOST protein plays a big role in the process of protein glycosylation. As part of the OST complex it facilitates the attachment of oligosaccharides to specific asparagine residues of target proteins in the ER. This glycosylation process helps with protein folding and stability and is important for the functionality and longevity of the proteins. Alterations in this system can affect protein structure and function illustrating DDOST's critical role in ensuring protein integrity and cellular homeostasis.

Pathways

DDOST participates rigorously in the N-glycosylation pathway. It interacts with key enzymes involved in the transfer of glycan moieties to nascent polypeptides which is necessary for proper cellular function. The protein also works closely with ribophorin and other subunits of the OST complex. While directly part of this glycosylation pathway its activity indirectly impacts various cellular signaling pathways due to its role in modifying and folding membrane-bound and secreted proteins.

Defects in DDOST relate to congenital disorders of glycosylation. Mutations in this protein can lead to improper glycosylation causing a range of symptoms from developmental delays to neurological issues. Also changes in glycosylation patterns have associations with cancer progression. Beyond DDOST itself glycosylation often affects other key proteins contributing to these conditions such as integrins and cadherins especially in the context of cell adhesion and signaling.
style
left-column

Specifications

Form

Liquid

style
left-column

General info

Function

Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation (PubMed : 31831667). N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity (By similarity). Required for the assembly of both SST3A- and SS3B-containing OST complexes (PubMed : 22467853).

Sequence similarities

Belongs to the DDOST 48 kDa subunit family.

style
left-column

Product protocols

style
left-column

Target data

Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation (PubMed : 31831667). N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity (By similarity). Required for the assembly of both SST3A- and SS3B-containing OST complexes (PubMed : 22467853).
See full target information DDOST
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com