Recombinant Human DDX39 protein (denatured) is a Human Fragment protein, in the 1 to 249 aa range, expressed in Escherichia coli, with >80% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >80% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S E F M A E Q D V E N D L L D Y D E E E E P Q A P Q E S T P A P P K K D I K G S Y V S I H S S G F R D F L L K P E L L R A I V D C G F E H P S E V Q H E C I P Q A I L G M D V L C Q A K S G M G K T A V F V L A T L Q Q I E P V N G Q V T V L V M C H T R E L A F Q I S K E Y E R F S K Y M P S V K V S V F F G G L S I K K D E E V L K K N C P H V V V G T P G R I L A L V R N R S F S L K N V K H F V L D E C D K M L E Q L D M R R D V Q E I F R L T P H E K Q C M M F S A T L S K D I R P V C R K F M Q D P M E V F
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Isoform 1. Involved in pre-mRNA splicing. Required for the export of mRNA out of the nucleus.
Alternative names
DDX39, DDX39A, ATP-dependent RNA helicase DDX39A, DEAD box protein 39, Nuclear RNA helicase URH49
Recommended products
Recombinant Human DDX39 protein (denatured) is a Human Fragment protein, in the 1 to 249 aa range, expressed in Escherichia coli, with >80% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >80% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- O00148-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S E F M A E Q D V E N D L L D Y D E E E E P Q A P Q E S T P A P P K K D I K G S Y V S I H S S G F R D F L L K P E L L R A I V D C G F E H P S E V Q H E C I P Q A I L G M D V L C Q A K S G M G K T A V F V L A T L Q Q I E P V N G Q V T V L V M C H T R E L A F Q I S K E Y E R F S K Y M P S V K V S V F F G G L S I K K D E E V L K K N C P H V V V G T P G R I L A L V R N R S F S L K N V K H F V L D E C D K M L E Q L D M R R D V Q E I F R L T P H E K Q C M M F S A T L S K D I R P V C R K F M Q D P M E V F
- Accession
- O00148
- Protein length
- Fragment
- Predicted molecular weight
- 31 kDa
- Amino acids
- 1 to 249
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Isoform 1
- Sequence similarities
Belongs to the DEAD box helicase family. DECD subfamily.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The DDX39 protein also known as DDX39A or BAT1 belongs to the DEAD box family of proteins and has a molecular mass of approximately 49 kDa. Mechanically DDX39 functions as an RNA helicase which plays a critical role in the unwinding of RNA secondary structures during various RNA metabolic processes. The protein facilitates RNA splicing transport and translation by interacting with these fundamental cellular mechanisms. DDX39 is widely expressed in various tissues including liver heart and brain highlighting its importance across different biological systems.
- Biological function summary
The multifunctional attributes of DDX39 contribute significantly to RNA processing events. The protein serves as an integral part of the TREX (transcription/export) complex which mediates the export of mRNA from the nucleus to the cytoplasm. By unwinding RNA DDX39 ensures proper mRNA maturation and transport essential for accurate gene expression. The involvement of DDX39 in the TREX complex highlights its central role in maintaining cellular homeostasis.
- Pathways
DDX39 participates actively in gene expression and mRNA processing pathways. The protein's role in the TREX complex links it to the mRNA export pathway important for synthesizing proteins and cell cycle progression. Through its activities DDX39 interacts with related proteins such as ALYREF and UAP56 coordinating the transfer of mRNA. These interactions highlight its collaborative function within complex cellular processes.
- Associated diseases and disorders
DDX39 has connections to cancer including breast and lung cancer where altered mRNA processing contributes to tumor progression. The protein associates closely with other mRNA processing components like ALYREF where dysregulated interactions might influence cancer cell proliferation. Additionally DDX39 abnormalities have associations with spinal muscular atrophy where its involvement in RNA processing affects motor neuron health. This links DDX39 through its complex roles in both cancer and neurodegenerative disorders.
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1 product image
SDS-PAGE - Recombinant Human DDX39 protein (denatured) (ab181916)
15% SDS-PAGE analysis of ab181916 (3μg).
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