Recombinant Human DDX6 protein is a Human Full Length protein, in the 1 to 483 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
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- PloS one 13:e02037082018The DEAD-box RNA-binding protein DDX6 regulates parental RNA decay for cellular reprogramming to pluripotency.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaisuke Kami et. al.PubMed 30273347
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M S T A R T E N P V I M G L S S Q N G Q L R G P V K P T G G P G G G G T Q T Q Q Q M N Q L K N T N T I N N G T Q Q Q A Q S M T T T I K P G D D W K K T L K L P P K D L R I K T S D V T S T K G N E F E D Y C L K R E L L M G I F E M G W E K P S P I Q E E S I P I A L S G R D I L A R A K N G T G K S G A Y L I P L L E R L D L K K D N I Q A M V I V P T R E L A L Q V S Q I C I Q V S K H M G G A K V M A T T G G T N L R D D I M R L D D T V H V V I A T P G R I L D L I K K G V A K V D H V Q M I V L D E A D K L L S Q D F V Q I M E D I I L T L P K N R Q I L L Y S A T F P L S V Q K F M N S H L Q K P Y E I N L M E E L T L K G V T Q Y Y A Y V T E R Q K V H C L N T L F S R L Q I N Q S I I F C N S S Q R V E L L A K K I S Q L G Y S C F Y I H A K M R Q E H R N R V F H D F R N G L C R N L V C T D L F T R G I D I Q A V N V V I N F D F P K L A E T Y L H R I G R S G R F G H L G L A I N L I T Y D D R F N L K S I E E Q L G T E I K P I P S N I D K S L Y V A E Y H S E P V E D E K P
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes Recombinant protein |
Target data
Function
Essential for the formation of P-bodies, cytosolic membrane-less ribonucleoprotein granules involved in RNA metabolism through the coordinated storage of mRNAs encoding regulatory functions (PubMed:25995375, PubMed:27342281, PubMed:31422817). Plays a role in P-bodies to coordinate the storage of translationally inactive mRNAs in the cytoplasm and prevent their degradation (PubMed:27342281). In the process of mRNA degradation, plays a role in mRNA decapping (PubMed:16364915). Blocks autophagy in nutrient-rich conditions by repressing the expression of ATG-related genes through degradation of their transcripts (PubMed:26098573).
Alternative names
HLR2, RCK, DDX6, Probable ATP-dependent RNA helicase DDX6, ATP-dependent RNA helicase p54, DEAD box protein 6, Oncogene RCK
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Recombinant Human DDX6 protein is a Human Full Length protein, in the 1 to 483 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- P26196-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.3% Glutathione
Sequence info
Amino acid sequence
- M S T A R T E N P V I M G L S S Q N G Q L R G P V K P T G G P G G G G T Q T Q Q Q M N Q L K N T N T I N N G T Q Q Q A Q S M T T T I K P G D D W K K T L K L P P K D L R I K T S D V T S T K G N E F E D Y C L K R E L L M G I F E M G W E K P S P I Q E E S I P I A L S G R D I L A R A K N G T G K S G A Y L I P L L E R L D L K K D N I Q A M V I V P T R E L A L Q V S Q I C I Q V S K H M G G A K V M A T T G G T N L R D D I M R L D D T V H V V I A T P G R I L D L I K K G V A K V D H V Q M I V L D E A D K L L S Q D F V Q I M E D I I L T L P K N R Q I L L Y S A T F P L S V Q K F M N S H L Q K P Y E I N L M E E L T L K G V T Q Y Y A Y V T E R Q K V H C L N T L F S R L Q I N Q S I I F C N S S Q R V E L L A K K I S Q L G Y S C F Y I H A K M R Q E H R N R V F H D F R N G L C R N L V C T D L F T R G I D I Q A V N V V I N F D F P K L A E T Y L H R I G R S G R F G H L G L A I N L I T Y D D R F N L K S I E E Q L G T E I K P I P S N I D K S L Y V A E Y H S E P V E D E K P
- Accession
- P26196
- Protein length
- Full Length
- Predicted molecular weight
- 79.24 kDa
- Amino acids
- 1 to 483
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Essential for the formation of P-bodies, cytosolic membrane-less ribonucleoprotein granules involved in RNA metabolism through the coordinated storage of mRNAs encoding regulatory functions (PubMed:25995375, PubMed:27342281, PubMed:31422817). Plays a role in P-bodies to coordinate the storage of translationally inactive mRNAs in the cytoplasm and prevent their degradation (PubMed:27342281). In the process of mRNA degradation, plays a role in mRNA decapping (PubMed:16364915). Blocks autophagy in nutrient-rich conditions by repressing the expression of ATG-related genes through degradation of their transcripts (PubMed:26098573).
- Sequence similarities
Belongs to the DEAD box helicase family. DDX6/DHH1 subfamily.
- Post-translational modifications
Sumoylated (PubMed:26184334).
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
DDX6 also known as RCK or p54 is an RNA helicase with a molecular mass of approximately 54 kDa. This protein is primarily located in the cytoplasm and is involved in processes such as mRNA degradation and translational repression. DDX6 acts mechanically by unwinding RNA molecules which facilitates various RNA processing events critical for cellular function. Its expression occurs broadly across different tissues indicating its significant role in diverse cellular operations.
- Biological function summary
DDX6 functions as part of the RNA-induced silencing complex (RISC) and is involved in messenger ribonucleoprotein (mRNP) granule formation. These granules include P-bodies and stress granules which are essential for mRNA metabolism and storage. DDX6 collaborates with other proteins such as EDC3 and DCP1 to regulate mRNA turnover and decay. Its role in post-transcriptional control impacts gene expression regulation at multiple levels.
- Pathways
The involvement of DDX6 in the RNA interference (RNAi) and nonsense-mediated mRNA decay (NMD) pathways is significant. In the RNAi pathway it interacts with proteins like AGO2 to mediate gene silencing by microRNAs. DDX6 also participates in the NMD pathway by targeting aberrant mRNAs for degradation assisting proteins like UPF1. These pathways demonstrate the important role of DDX6 in maintaining cellular RNA homeostasis and preventing the accumulation of defective RNAs.
- Associated diseases and disorders
DDX6 has been linked to cancer and neurodegenerative diseases. Its interaction with cancer-related proteins like AURKA suggests a role in tumorigenesis where it may affect cell proliferation and survival. Additionally DDX6's involvement in neurodegenerative disorders has been observed where dysregulation of its associated RNA processes may contribute to disease pathology. Understanding these connections provides insight into potential therapeutic targets for related diseases.
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SDS-PAGE - Recombinant Human DDX6 protein (ab114574)
12.5% SDS-PAGE Stained with Coomassie Blue
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