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AB114574

Recombinant Human DDX6 protein (GST tag N-Terminus)

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(1 Publication)

Recombinant Human DDX6 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 483 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

HLR2, RCK, DDX6, Probable ATP-dependent RNA helicase DDX6, ATP-dependent RNA helicase p54, DEAD box protein 6, Oncogene RCK

1 Images
SDS-PAGE - Recombinant Human DDX6 protein (GST tag N-Terminus) (AB114574)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human DDX6 protein (GST tag N-Terminus) (AB114574)

12.5% SDS-PAGE Stained with Coomassie Blue

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

SDS-PAGE, ELISA, WB

applications

Biologically active

No

Accession

P26196

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.3% Glutathione

storage-buffer

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Complementary Products

Primary Antibodies

AB307418

Anti-DDX6 antibody [EPR27016-10]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX6 antibody [EPR27016-10] (AB307418)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Proteins & Peptides

AB152728

Recombinant Human ZEB1 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human ZEB1 protein (GST tag N-Terminus) (AB152728)

  • 0
  • 0
Proteins & Peptides

AB240764

Recombinant Human LOX protein (His tag)

SDS-PAGE - Recombinant Human LOX protein (His tag) (AB240764)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Recombinant protein</p>" } } }
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Sequence info

[{"sequence":"MSTARTENPVIMGLSSQNGQLRGPVKPTGGPGGGGTQTQQQMNQLKNTNTINNGTQQQAQSMTTTIKPGDDWKKTLKLPPKDLRIKTSDVTSTKGNEFEDYCLKRELLMGIFEMGWEKPSPIQEESIPIALSGRDILARAKNGTGKSGAYLIPLLERLDLKKDNIQAMVIVPTRELALQVSQICIQVSKHMGGAKVMATTGGTNLRDDIMRLDDTVHVVIATPGRILDLIKKGVAKVDHVQMIVLDEADKLLSQDFVQIMEDIILTLPKNRQILLYSATFPLSVQKFMNSHLQKPYEINLMEELTLKGVTQYYAYVTERQKVHCLNTLFSRLQINQSIIFCNSSQRVELLAKKISQLGYSCFYIHAKMRQEHRNRVFHDFRNGLCRNLVCTDLFTRGIDIQAVNVVINFDFPKLAETYLHRIGRSGRFGHLGLAINLITYDDRFNLKSIEEQLGTEIKPIPSNIDKSLYVAEYHSEPVEDEKP","proteinLength":"Full Length","predictedMolecularWeight":"79.24 kDa","actualMolecularWeight":null,"aminoAcidEnd":483,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P26196","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DDX6 also known as RCK or p54 is an RNA helicase with a molecular mass of approximately 54 kDa. This protein is primarily located in the cytoplasm and is involved in processes such as mRNA degradation and translational repression. DDX6 acts mechanically by unwinding RNA molecules which facilitates various RNA processing events critical for cellular function. Its expression occurs broadly across different tissues indicating its significant role in diverse cellular operations.
Biological function summary

DDX6 functions as part of the RNA-induced silencing complex (RISC) and is involved in messenger ribonucleoprotein (mRNP) granule formation. These granules include P-bodies and stress granules which are essential for mRNA metabolism and storage. DDX6 collaborates with other proteins such as EDC3 and DCP1 to regulate mRNA turnover and decay. Its role in post-transcriptional control impacts gene expression regulation at multiple levels.

Pathways

The involvement of DDX6 in the RNA interference (RNAi) and nonsense-mediated mRNA decay (NMD) pathways is significant. In the RNAi pathway it interacts with proteins like AGO2 to mediate gene silencing by microRNAs. DDX6 also participates in the NMD pathway by targeting aberrant mRNAs for degradation assisting proteins like UPF1. These pathways demonstrate the important role of DDX6 in maintaining cellular RNA homeostasis and preventing the accumulation of defective RNAs.

DDX6 has been linked to cancer and neurodegenerative diseases. Its interaction with cancer-related proteins like AURKA suggests a role in tumorigenesis where it may affect cell proliferation and survival. Additionally DDX6's involvement in neurodegenerative disorders has been observed where dysregulation of its associated RNA processes may contribute to disease pathology. Understanding these connections provides insight into potential therapeutic targets for related diseases.
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Specifications

Form

Liquid

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General info

Function

Essential for the formation of P-bodies, cytosolic membrane-less ribonucleoprotein granules involved in RNA metabolism through the coordinated storage of mRNAs encoding regulatory functions (PubMed : 25995375, PubMed : 27342281, PubMed : 31422817). Plays a role in P-bodies to coordinate the storage of translationally inactive mRNAs in the cytoplasm and prevent their degradation (PubMed : 27342281). In the process of mRNA degradation, plays a role in mRNA decapping (PubMed : 16364915). Blocks autophagy in nutrient-rich conditions by repressing the expression of ATG-related genes through degradation of their transcripts (PubMed : 26098573).

Sequence similarities

Belongs to the DEAD box helicase family. DDX6/DHH1 subfamily.

Post-translational modifications

Sumoylated (PubMed:26184334).

Subcellular localisation

Nucleus

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Product protocols

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Target data

Essential for the formation of P-bodies, cytosolic membrane-less ribonucleoprotein granules involved in RNA metabolism through the coordinated storage of mRNAs encoding regulatory functions (PubMed : 25995375, PubMed : 27342281, PubMed : 31422817). Plays a role in P-bodies to coordinate the storage of translationally inactive mRNAs in the cytoplasm and prevent their degradation (PubMed : 27342281). In the process of mRNA degradation, plays a role in mRNA decapping (PubMed : 16364915). Blocks autophagy in nutrient-rich conditions by repressing the expression of ATG-related genes through degradation of their transcripts (PubMed : 26098573).
See full target information DDX6
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PloS one 13:e0203708 PubMed30273347

2018

The DEAD-box RNA-binding protein DDX6 regulates parental RNA decay for cellular reprogramming to pluripotency.

Applications

Unspecified application

Species

Unspecified reactive species

Daisuke Kami,Tomoya Kitani,Akihiro Nakamura,Naoki Wakui,Rena Mizutani,Masahito Ohue,Fuyuki Kametani,Nobuyoshi Akimitsu,Satoshi Gojo
View all publications
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Product promise

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