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AB283431

Recombinant Human Decorin protein (Active)

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Recombinant Human Decorin protein (Active) is a Human Full Length protein, in the 31 to 359 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for SDS-PAGE, Mass Spec, HPLC, Biological Activity, FuncS.

View Alternative Names

SLRR1B, DCN, Decorin, Bone proteoglycan II, PG-S2, PG40

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Mass Spectrometry - Recombinant Human Decorin protein (Active) (AB283431)
  • Mass Spec

Lab

Mass Spectrometry - Recombinant Human Decorin protein (Active) (AB283431)

Mass determination by ESI-TOF.

Predicted MW is 36383.70 Da (+/- 10 Da by ESI-TOF). Observed MW is 36259.04 Da & 36391.79 Da.

Biochemical assay - Recombinant Human Decorin protein (Active) (AB283431)
  • Biochemical assay

Supplier Data

Biochemical assay - Recombinant Human Decorin protein (Active) (AB283431)

Fully biologically active by measuring its ability to modulate collagen fibrillogenesis. At concentration above 10 ug/ml, recombinant human Decorin can significantly delay the rate of type I collagen fibrillogenesis (turbidity).

HPLC - Recombinant Human Decorin protein (Active) (AB283431)
  • HPLC

Lab

HPLC - Recombinant Human Decorin protein (Active) (AB283431)

HPLC analysis of ab283431

SDS-PAGE - Recombinant Human Decorin protein (Active) (AB283431)
  • SDS-PAGE

Lab

SDS-PAGE - Recombinant Human Decorin protein (Active) (AB283431)

SDS-PAGE analysis of ab283431

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

<0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

SDS-PAGE, Mass Spec, FuncS, Biological Activity, HPLC

applications

Biologically active

Yes

Biological activity

Fully biologically active by measuring its ability to modulate collagen fibrillogenesis. At concentration above 10 μg/ml, recombinant human Decorin can significantly delay the rate of type I collagen fibrillogenesis (turbidity).

Accession

P07585

Animal free

Yes

Carrier free

No

Species

Human

Reconstitution

Reconstitute in PBS

Storage buffer

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Biological Activity": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" } } }
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Sequence info

[{"sequence":"DEASGIGPEVPDDRDFEPSLGPVCPFRCQCHLRVVQCSDLGLDKVPKDLPPDTTLLDLQNNKITEIKDGDFKNLKNLHALILVNNKISKVSPGAFTPLVKLERLYLSKNQLKELPEKMPKTLQELRAHENEITKVRKVTFNGLNQMIVIELGTNPLKSSGIENGAFQGMKKLSYIRIADTNITSIPQGLPPSLTELHLDGNKISRVDAASLKGLNNLAKLGLSFNSISAVDNGSLANTPHLRELHLDNNKLTRVPGGLAEHKYIQVVYLHNNNISVVGSSDFCPPGHNTKKASYSGVSLFSNPVQYWEIQPSTFRCVYVRSAIQLGNYK","proteinLength":"Full Length","predictedMolecularWeight":"36.38 kDa","actualMolecularWeight":"36.26 kDa","aminoAcidEnd":359,"aminoAcidStart":31,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P07585","tags":[]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
True
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Specifications

Form

Lyophilized

Additional notes

Purity by HPLC =95%

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General info

Function

May affect the rate of fibrils formation.

Sequence similarities

Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.

Post-translational modifications

The attached glycosaminoglycan chain can be either chondroitin sulfate or dermatan sulfate depending upon the tissue of origin.

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Product protocols

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Target data

May affect the rate of fibrils formation.
See full target information DCN
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