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Recombinant Human Decorin protein (Active) is a Human Full Length protein, in the 31 to 359 aa range, expressed in HEK 293, with >95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, MS, HPLC, Biological Activity.

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Images

SDS-PAGE - Recombinant Human Decorin protein (Active) (AB283431), expandable thumbnail
  • Mass Spectrometry - Recombinant Human Decorin protein (Active) (AB283431), expandable thumbnail
  • HPLC - Recombinant Human Decorin protein (Active) (AB283431), expandable thumbnail
  • Biochemical assay - Recombinant Human Decorin protein (Active) (AB283431), expandable thumbnail

Key facts

Purity
>95% SDS-PAGE
Endotoxin level
<= 0.005 EU/µg
Expression system
HEK 293 cells
Tags
Tag free
Applications
SDS-PAGE, MS, HPLC, Biological Activity
Biologically active
Yes

Amino acid sequence

D E A S G I G P E V P D D R D F E P S L G P V C P F R C Q C H L R V V Q C S D L G L D K V P K D L P P D T T L L D L Q N N K I T E I K D G D F K N L K N L H A L I L V N N K I S K V S P G A F T P L V K L E R L Y L S K N Q L K E L P E K M P K T L Q E L R A H E N E I T K V R K V T F N G L N Q M I V I E L G T N P L K S S G I E N G A F Q G M K K L S Y I R I A D T N I T S I P Q G L P P S L T E L H L D G N K I S R V D A A S L K G L N N L A K L G L S F N S I S A V D N G S L A N T P H L R E L H L D N N K L T R V P G G L A E H K Y I Q V V Y L H N N N I S V V G S S D F C P P G H N T K K A S Y S G V S L F S N P V Q Y W E I Q P S T F R C V Y V R S A I Q L G N Y K

Reactivity data

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Application
MS
Reactivity
Reacts
Dilution info
-
Notes

-

Application
HPLC
Reactivity
Reacts
Dilution info
-
Notes

-

Application
Biological Activity
Reactivity
Reacts
Dilution info
-
Notes

-

Target data

Function

May affect the rate of fibrils formation.

Alternative names

Recommended products

Recombinant Human Decorin protein (Active) is a Human Full Length protein, in the 31 to 359 aa range, expressed in HEK 293, with >95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, MS, HPLC, Biological Activity.

Key facts

Purity
>95% SDS-PAGE
Endotoxin level
<= 0.005 EU/µg
Expression system
HEK 293 cells
Applications
SDS-PAGE, MS, HPLC, Biological Activity
Biological activity
Fully biologically active by measuring its ability to modulate collagen fibrillogenesis. At concentration above 10 μg/ml, recombinant human Decorin can significantly delay the rate of type I collagen fibrillogenesis (turbidity).
Accession
P07585-1
Animal free
Yes
Species
Human
Reconstitution
Reconstitute in PBS
Concentration
Loading...
Storage buffer

pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

Sequence info

Amino acid sequence

D E A S G I G P E V P D D R D F E P S L G P V C P F R C Q C H L R V V Q C S D L G L D K V P K D L P P D T T L L D L Q N N K I T E I K D G D F K N L K N L H A L I L V N N K I S K V S P G A F T P L V K L E R L Y L S K N Q L K E L P E K M P K T L Q E L R A H E N E I T K V R K V T F N G L N Q M I V I E L G T N P L K S S G I E N G A F Q G M K K L S Y I R I A D T N I T S I P Q G L P P S L T E L H L D G N K I S R V D A A S L K G L N N L A K L G L S F N S I S A V D N G S L A N T P H L R E L H L D N N K L T R V P G G L A E H K Y I Q V V Y L H N N N I S V V G S S D F C P P G H N T K K A S Y S G V S L F S N P V Q Y W E I Q P S T F R C V Y V R S A I Q L G N Y K
Accession
P07585
Protein length
Full Length
Predicted molecular weight
36.38 kDa
Amino acids
31 to 359
Nature
Recombinant

Specifications

Form
Lyophilized
Additional notes

Purity by HPLC =95%

General info

Function

May affect the rate of fibrils formation.

Sequence similarities

Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.

Post-translational modifications

The attached glycosaminoglycan chain can be either chondroitin sulfate or dermatan sulfate depending upon the tissue of origin.

Storage

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • SDS-PAGE - Recombinant Human Decorin protein (Active) (ab283431), expandable thumbnail

    SDS-PAGE - Recombinant Human Decorin protein (Active) (ab283431)

    SDS-PAGE analysis of ab283431

  • Mass Spectrometry - Recombinant Human Decorin protein (Active) (ab283431), expandable thumbnail

    Mass Spectrometry - Recombinant Human Decorin protein (Active) (ab283431)

    Mass determination by ESI-TOF.

    Predicted MW is 36383.70 Da (+/- 10 Da by ESI-TOF). Observed MW is 36259.04 Da & 36391.79 Da.

  • HPLC - Recombinant Human Decorin protein (Active) (ab283431), expandable thumbnail

    HPLC - Recombinant Human Decorin protein (Active) (ab283431)

    HPLC analysis of ab283431

  • Biochemical assay - Recombinant Human Decorin protein (Active) (ab283431), expandable thumbnail

    Biochemical assay - Recombinant Human Decorin protein (Active) (ab283431)

    Fully biologically active by measuring its ability to modulate collagen fibrillogenesis.
    At concentration above 10 ug/ml, recombinant human Decorin can significantly delay the rate of type I collagen fibrillogenesis (turbidity).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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