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AB153463

Recombinant Human DENND1A protein (GST tag N-Terminus)

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Recombinant Human DENND1A protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 559 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

FAM31A, KIAA1608, DENND1A, DENN domain-containing protein 1A, Connecdenn 1, Protein FAM31A, Connecdenn

1 Images
SDS-PAGE - Recombinant Human DENND1A protein (GST tag N-Terminus) (AB153463)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human DENND1A protein (GST tag N-Terminus) (AB153463)

ab153463 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, WB

applications

Biologically active

No

Accession

Q8TEH3

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSRIKQNPETTFEVYVEVAYPRTGGTLSDPEVQRQFPEDYSDQEVLQTLTKFCFPFYVDSLTVSQVGQNFTFVLTDIDSKQRFGFCRLSSGAKSCFCILSYLPWFEVFYKLLNILADYTTKRQENQWNELLETLHKLPIPDPGVSVHLSVHSYFTVPDTRELPSIPENRNLTEYFVAVDVNNMLHLYASMLYERRILIICSKLSTLTACIHGSAAMLYPMYWQHVYIPVLPPHLLDYCCAPMPYLIGIHLSLMEKVRNMALDDVVILNVDTNTLETPFDDLQSLPNDVISSLKNRLKKVSTTTGDGVARAFLKAQAAFFGSYRNALKIEPEEPITFCEEAFVSHYRSGAMRQFLQNATQLQLFKQFIDGRLDLLNSGEGFSDVFEEEINMGEYAGSDKLYHQWLSTVRKGSGAILNTVKTKANPAMKTVYKFAKDHAKMGIKEVKNRLKQKDIAENGCAPTPEEQLPKTAPSPLVEAKDPKLREDRRPITVHFGQVRPPRPHVVKRPKSNIAVEGRRTSVPSPEQNTIATPATLHILQKSITHFAAKFPTRGWTSSSH","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":559,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q8TEH3","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DENND1A also known as DENN domain-containing protein 1A functions as a guanine nucleotide exchange factor (GEF) with a recognized molecular mass of approximately 115 kDa. It facilitates the activation of Rab proteins which are small GTPases involved in the regulation of intracellular vesicular transport. DENND1A is expressed in various tissues with significant levels found in the brain liver and kidneys indicating its diverse role in cellular processes.
Biological function summary

DENND1A plays an important part in regulating endocytic membrane traffic. It forms part of the multi-protein complex that is responsible for vesicle budding delivery and membrane fusion. This protein aids in mediating the interactions between vesicles and their target membranes ensuring precise and timely transport of cellular materials. Its role in cellular transport highlights the importance of maintaining cellular homeostasis and function.

Pathways

DENND1A participates significantly in the endocytic and exocytic pathways. These pathways control the internalization and secretion of cellular substances important for cellular communication and nutrient uptake. DENND1A influences the function of the Rab GTPases which play a central role in these pathways. Through this interaction DENND1A helps in maintaining the balance between vesicle trafficking and signaling pathways.

DENND1A has been linked to conditions such as polycystic ovary syndrome (PCOS) and certain neurological disorders. Its interaction with proteins involved in these diseases such as insulin receptor substrates in PCOS suggests that alterations in DENND1A expression or function could disrupt normal cellular transport and signaling mechanisms contributing to disease pathogenesis. Understanding its role in these disorders could offer insight into potential therapeutic targets.
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Specifications

Form

Liquid

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General info

Function

Guanine nucleotide exchange factor (GEF) regulating clathrin-mediated endocytosis through RAB35 activation. Promotes the exchange of GDP to GTP, converting inactive GDP-bound RAB35 into its active GTP-bound form. Regulates clathrin-mediated endocytosis of synaptic vesicles and mediates exit from early endosomes (PubMed : 20154091, PubMed : 20937701). Binds phosphatidylinositol-phosphates (PtdInsPs), with some preference for PtdIns(3)P (By similarity).

Post-translational modifications

Phosphorylated on serine and/or threonine in an Akt-dependent manner. Phosphorylation probably regulates the guanine nucleotide exchange factor (GEF) activity, possibly by disrupting an intramolecular interaction between the DENN domain and the C-terminus of the protein, thereby relieving the autoinhibition.

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Product protocols

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Target data

Guanine nucleotide exchange factor (GEF) regulating clathrin-mediated endocytosis through RAB35 activation. Promotes the exchange of GDP to GTP, converting inactive GDP-bound RAB35 into its active GTP-bound form. Regulates clathrin-mediated endocytosis of synaptic vesicles and mediates exit from early endosomes (PubMed : 20154091, PubMed : 20937701). Binds phosphatidylinositol-phosphates (PtdInsPs), with some preference for PtdIns(3)P (By similarity).
See full target information DENND1A
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