Recombinant Human Deptor protein is a Human Full Length protein, in the 1 to 409 aa range, expressed in Wheat germ and suitable for ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
M E E G G S T G S A G S D S S T S G S G G A Q Q R E L E R M A E V L V T G E Q L R L R L H E E K V I K D R R H H L K T Y P N C F V A K E L I D W L I E H K E A S D R E T A I K L M Q K L A D R G I I H H V C D E H K E F K D V K L F Y R F R K D D G T F P L D N E V K A F M R G Q R L Y E K L M S P E N T L L Q P R E E E G V K Y E R T F M A S E F L D W L V Q E G E A T T R K E A E Q L C H R L M E H G I I Q H V S S K H P F V D S N L L Y Q F R M N F R R R R R L M E L L N E K S P S S Q E T H D S P F C L R K Q S H D N R K S T S F M S V S P S K E I K T V S A V R R S S M S S C G S S G Y F S S S P T L S S S P P V L C N P K S V L K R P V T S E E L L T P G A P Y A R K T F T I V G D A V G W G F V V R G S K P C H I Q A V D P S G P A A A A G M K V C Q F V V S V N G L N V L H V D Y R T V N N L I L T G P R T I V M E V M E E L E C
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Negative regulator of the mTORC1 and mTORC2 complexes: inhibits the protein kinase activity of MTOR, thereby inactivating both complexes (PubMed:19446321, PubMed:22017875, PubMed:22017876, PubMed:22017877, PubMed:25936805, PubMed:29382726, PubMed:34519268, PubMed:34519269). DEPTOR inhibits mTORC1 and mTORC2 to induce autophagy (PubMed:22017875, PubMed:22017876, PubMed:22017877). In contrast to AKT1S1/PRAS40, only partially inhibits mTORC1 activity (PubMed:34519268, PubMed:34519269).
Alternative names
DEPDC6, DEPTOR, DEP domain-containing mTOR-interacting protein, hDEPTOR, DEP domain-containing protein 6
Recommended products
Recombinant Human Deptor protein is a Human Full Length protein, in the 1 to 409 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- Q8TB45-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M E E G G S T G S A G S D S S T S G S G G A Q Q R E L E R M A E V L V T G E Q L R L R L H E E K V I K D R R H H L K T Y P N C F V A K E L I D W L I E H K E A S D R E T A I K L M Q K L A D R G I I H H V C D E H K E F K D V K L F Y R F R K D D G T F P L D N E V K A F M R G Q R L Y E K L M S P E N T L L Q P R E E E G V K Y E R T F M A S E F L D W L V Q E G E A T T R K E A E Q L C H R L M E H G I I Q H V S S K H P F V D S N L L Y Q F R M N F R R R R R L M E L L N E K S P S S Q E T H D S P F C L R K Q S H D N R K S T S F M S V S P S K E I K T V S A V R R S S M S S C G S S G Y F S S S P T L S S S P P V L C N P K S V L K R P V T S E E L L T P G A P Y A R K T F T I V G D A V G W G F V V R G S K P C H I Q A V D P S G P A A A A G M K V C Q F V V S V N G L N V L H V D Y R T V N N L I L T G P R T I V M E V M E E L E C
- Accession
- Q8TB45
- Protein length
- Full Length
- Amino acids
- 1 to 409
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Negative regulator of the mTORC1 and mTORC2 complexes: inhibits the protein kinase activity of MTOR, thereby inactivating both complexes (PubMed:19446321, PubMed:22017875, PubMed:22017876, PubMed:22017877, PubMed:25936805, PubMed:29382726, PubMed:34519268, PubMed:34519269). DEPTOR inhibits mTORC1 and mTORC2 to induce autophagy (PubMed:22017875, PubMed:22017876, PubMed:22017877). In contrast to AKT1S1/PRAS40, only partially inhibits mTORC1 activity (PubMed:34519268, PubMed:34519269).
- Post-translational modifications
Phosphorylation weakens interaction with MTOR within mTORC1 and mTORC2 (PubMed:19446321). Phosphorylated at Ser-286, Ser-287 and Ser-291 in response to mitogenic stimulation by MTOR: DEPTOR is either directly phosphorylated by MTOR or indirectly via proteins kinases that are activated by MTOR, such as CK1/CSNK1A1 (PubMed:22017875, PubMed:22017876, PubMed:22017877). Phosphorylation at Ser-286, Ser-287 and Ser-291 promotes ubiquitination by the SCF(BTRC) complex, followed by degradation (PubMed:22017875, PubMed:22017876, PubMed:22017877). Phosphorylation at Ser-235 by MAPK3/ERK1 promotes deubiquitination by USP7, enhancing its stability (PubMed:35216969). Phosphorylation at Tyr-289 by SYK impairs its interaction with MTOR, promoting mTORC1 and mTORC2 signaling (PubMed:34634301).
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
DEPTOR also known as DEP domain-containing mTOR-interacting protein functions as a subunit of the mTOR (mechanistic Target of Rapamycin) complexes. This protein has a molecular weight of approximately 48 kDa and is expressed extensively in tissues such as skeletal muscle adipose tissue and the brain. DEPTOR directly interacts with the mTOR complexes modulating their activity. Its exact mechanical role involves binding to mTOR and influencing the assembly and function of mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2).
- Biological function summary
DEPTOR is important for the regulation of cell growth and metabolism. It is a component of the mTORC1 and mTORC2 two key protein complexes that control cell proliferation survival and autophagy. DEPTOR acts as an inhibitor within these complexes reducing their activity. By regulating the mTOR signaling pathway it helps balance anabolic and catabolic processes within the cell. Without proper DEPTOR function cells may grow unchecked leading to various issues including metabolic dysregulation.
- Pathways
DEPTOR plays a significant role in the mTOR signaling pathway and the PI3K/AKT pathway. In the mTOR pathway DEPTOR controls the activity of mTORC1 and mTORC2 which are essential for translating signaling into cellular response. The PI3K/AKT pathway also involves DEPTOR as it relates to insulin signaling and glucose homeostasis. Proteins such as AKT and S6K work in this complex to transmit signals impacting numerous cellular activities. The dynamic balance it maintains within these pathways is critical for normal cellular function and metabolism.
- Associated diseases and disorders
DEPTOR is associated with conditions such as cancer and type 2 diabetes. In cancers its reduced expression allows for unchecked mTOR activity driving tumor growth and survival. Conversely unregulated expression can lead to increased cell apoptosis. DEPTOR's dysregulation in the context of type 2 diabetes affects glucose metabolism through interactions with the PI3K/AKT pathway. It also interacts with proteins like IRS-1 influencing insulin sensitivity and leading to metabolic disorders. DEPTOR's regulation of these pathways suggests potential therapeutic targets in these diseases.
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1 product image
SDS-PAGE - Recombinant Human Deptor protein (ab163883)
ab163883 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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