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AB163883

Recombinant Human Deptor protein

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Recombinant Human Deptor protein is a Human Full Length protein, in the 1 to 409 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

DEPDC6, DEPTOR, DEP domain-containing mTOR-interacting protein, hDEPTOR, DEP domain-containing protein 6

1 Images
SDS-PAGE - Recombinant Human Deptor protein (AB163883)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Deptor protein (AB163883)

ab163883 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, ELISA

applications

Biologically active

No

Accession

Q8TB45

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MEEGGSTGSAGSDSSTSGSGGAQQRELERMAEVLVTGEQLRLRLHEEKVIKDRRHHLKTYPNCFVAKELIDWLIEHKEASDRETAIKLMQKLADRGIIHHVCDEHKEFKDVKLFYRFRKDDGTFPLDNEVKAFMRGQRLYEKLMSPENTLLQPREEEGVKYERTFMASEFLDWLVQEGEATTRKEAEQLCHRLMEHGIIQHVSSKHPFVDSNLLYQFRMNFRRRRRLMELLNEKSPSSQETHDSPFCLRKQSHDNRKSTSFMSVSPSKEIKTVSAVRRSSMSSCGSSGYFSSSPTLSSSPPVLCNPKSVLKRPVTSEELLTPGAPYARKTFTIVGDAVGWGFVVRGSKPCHIQAVDPSGPAAAAGMKVCQFVVSVNGLNVLHVDYRTVNNLILTGPRTIVMEVMEELEC","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":409,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q8TB45","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DEPTOR also known as DEP domain-containing mTOR-interacting protein functions as a subunit of the mTOR (mechanistic Target of Rapamycin) complexes. This protein has a molecular weight of approximately 48 kDa and is expressed extensively in tissues such as skeletal muscle adipose tissue and the brain. DEPTOR directly interacts with the mTOR complexes modulating their activity. Its exact mechanical role involves binding to mTOR and influencing the assembly and function of mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2).
Biological function summary

DEPTOR is important for the regulation of cell growth and metabolism. It is a component of the mTORC1 and mTORC2 two key protein complexes that control cell proliferation survival and autophagy. DEPTOR acts as an inhibitor within these complexes reducing their activity. By regulating the mTOR signaling pathway it helps balance anabolic and catabolic processes within the cell. Without proper DEPTOR function cells may grow unchecked leading to various issues including metabolic dysregulation.

Pathways

DEPTOR plays a significant role in the mTOR signaling pathway and the PI3K/AKT pathway. In the mTOR pathway DEPTOR controls the activity of mTORC1 and mTORC2 which are essential for translating signaling into cellular response. The PI3K/AKT pathway also involves DEPTOR as it relates to insulin signaling and glucose homeostasis. Proteins such as AKT and S6K work in this complex to transmit signals impacting numerous cellular activities. The dynamic balance it maintains within these pathways is critical for normal cellular function and metabolism.

DEPTOR is associated with conditions such as cancer and type 2 diabetes. In cancers its reduced expression allows for unchecked mTOR activity driving tumor growth and survival. Conversely unregulated expression can lead to increased cell apoptosis. DEPTOR's dysregulation in the context of type 2 diabetes affects glucose metabolism through interactions with the PI3K/AKT pathway. It also interacts with proteins like IRS-1 influencing insulin sensitivity and leading to metabolic disorders. DEPTOR's regulation of these pathways suggests potential therapeutic targets in these diseases.
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Specifications

Form

Liquid

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General info

Function

Negative regulator of the mTORC1 and mTORC2 complexes : inhibits the protein kinase activity of MTOR, thereby inactivating both complexes (PubMed : 19446321, PubMed : 22017875, PubMed : 22017876, PubMed : 22017877, PubMed : 25936805, PubMed : 29382726, PubMed : 34519268, PubMed : 34519269). DEPTOR inhibits mTORC1 and mTORC2 to induce autophagy (PubMed : 22017875, PubMed : 22017876, PubMed : 22017877). In contrast to AKT1S1/PRAS40, only partially inhibits mTORC1 activity (PubMed : 34519268, PubMed : 34519269).

Post-translational modifications

Phosphorylation weakens interaction with MTOR within mTORC1 and mTORC2 (PubMed:19446321). Phosphorylated at Ser-286, Ser-287 and Ser-291 in response to mitogenic stimulation by MTOR: DEPTOR is either directly phosphorylated by MTOR or indirectly via proteins kinases that are activated by MTOR, such as CK1/CSNK1A1 (PubMed:22017875, PubMed:22017876, PubMed:22017877). Phosphorylation at Ser-286, Ser-287 and Ser-291 promotes ubiquitination by the SCF(BTRC) complex, followed by degradation (PubMed:22017875, PubMed:22017876, PubMed:22017877). Phosphorylation at Ser-235 by MAPK3/ERK1 promotes deubiquitination by USP7, enhancing its stability (PubMed:35216969). Phosphorylation at Tyr-289 by SYK impairs its interaction with MTOR, promoting mTORC1 and mTORC2 signaling (PubMed:34634301).. Ubiquitinated; leading to proteasomal degradation (PubMed:22017875, PubMed:22017876, PubMed:22017877, PubMed:30080879, PubMed:33110214). Ubiquitination by the SCF(BTRC) and SCF(FBXW11) complexes following phosphorylation at Ser-286, Ser-287 and Ser-291 by MTOR, leads to its degradation by the proteasome (PubMed:22017875, PubMed:22017876, PubMed:22017877, PubMed:33110214). Deubiquitinated by OTUB1 in response to amino acid via a non-canonical mechanism, leading to DEPTOR stability (PubMed:29382726). Deubiquitinated by USP7 following phosphorylation at Ser-235, promoting its stability (PubMed:35216969).

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Product protocols

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Target data

Negative regulator of the mTORC1 and mTORC2 complexes : inhibits the protein kinase activity of MTOR, thereby inactivating both complexes (PubMed : 19446321, PubMed : 22017875, PubMed : 22017876, PubMed : 22017877, PubMed : 25936805, PubMed : 29382726, PubMed : 34519268, PubMed : 34519269). DEPTOR inhibits mTORC1 and mTORC2 to induce autophagy (PubMed : 22017875, PubMed : 22017876, PubMed : 22017877). In contrast to AKT1S1/PRAS40, only partially inhibits mTORC1 activity (PubMed : 34519268, PubMed : 34519269).
See full target information DEPTOR
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