Skip to main content

Recombinant Human DFFB/CAD protein is a Human Full Length protein, in the 1 to 338 aa range, expressed in Wheat germ and suitable for ELISA, WB.

Be the first to review this product! Submit a review

Images

SDS-PAGE - Recombinant Human DFFB/CAD protein (AB158274), expandable thumbnail

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, WB

Biologically active

No

Amino acid sequence

M L Q K P K S V K L R A L R S P R K F G V A G R S C Q E V L R K G C L R F Q L P E R G S R L C L Y E D G T E L T E D Y F P S V P D N A E L V L L T L G Q A W Q G Y V S D I R R F L S A F H E P Q V G L I Q A A Q Q L L C D E Q A P Q R Q R L L A D L L H N V S Q N I A A E T R A E D P P W F E G L E S R F Q S K S G Y L R Y S C E S R I R S Y L R E V S S Y P S T V G A E A Q E E F L R V L G S M C Q R L R S M Q Y N G S Y F D R G A K G G S R L C T P E G W F S C Q G P F D M D S C L S R H S I N P Y S N R E S R I L F S T W N L D H I I E K K R T I I P T L V E A I K E Q D G R E V D W E Y F Y G L L F T S E N L K L V H I V C H K K T T H K L N C D P S R I Y K P Q T R L K R K Q P V R K R Q

Reactivity data

Application

ELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Application

WB

Reactivity

Reacts

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology.

Alternative names

Recommended products

Recombinant Human DFFB/CAD protein is a Human Full Length protein, in the 1 to 338 aa range, expressed in Wheat germ and suitable for ELISA, WB.

Key facts

Expression system

Wheat germ

Applications

ELISA, WB

Accession
O76075-1
Animal free

No

Species

Human

Concentration
Loading...
Storage buffer

pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione

Sequence info

Amino acid sequence

M L Q K P K S V K L R A L R S P R K F G V A G R S C Q E V L R K G C L R F Q L P E R G S R L C L Y E D G T E L T E D Y F P S V P D N A E L V L L T L G Q A W Q G Y V S D I R R F L S A F H E P Q V G L I Q A A Q Q L L C D E Q A P Q R Q R L L A D L L H N V S Q N I A A E T R A E D P P W F E G L E S R F Q S K S G Y L R Y S C E S R I R S Y L R E V S S Y P S T V G A E A Q E E F L R V L G S M C Q R L R S M Q Y N G S Y F D R G A K G G S R L C T P E G W F S C Q G P F D M D S C L S R H S I N P Y S N R E S R I L F S T W N L D H I I E K K R T I I P T L V E A I K E Q D G R E V D W E Y F Y G L L F T S E N L K L V H I V C H K K T T H K L N C D P S R I Y K P Q T R L K R K Q P V R K R Q

Accession

O76075

Protein length

Full Length

Amino acids

1 to 338

Nature

Recombinant

Tags

GST tag N-Terminus

Specifications

Form

Liquid

General info

Function

Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology.

Subcellular localisation

Nucleus

Storage

Shipped at conditions

Dry Ice

Appropriate short-term storage conditions

-80°C

Appropriate long-term storage conditions

-80°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This product was previously labelled as Recombinant Human DFFB protein.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

DFFB also known as DNA fragmentation factor subunit beta or CAD is a protein with a molecular mass of approximately 40 kDa. It plays an important role in the apoptotic process by acting as a nuclease responsible for cleaving DNA into oligonucleosomal fragments. DFFB is found in various tissues expressing mainly in cells undergoing apoptosis. The interaction with its inhibitor DFFA prevents its activation until the apoptotic signal triggers the pathway for its release and activation resulting in DNA fragmentation.

Biological function summary

DFFB is critical in the execution phase of apoptosis which is the programmed cell death essential to maintaining cellular homeostasis and development. It forms a complex with DFFA its chaperone and inhibitor which regulates its nuclease activity. Apoptosis requires controlled DNA degradation activity which DFFB provides ensuring that cells dismantle DNA in a process safe for the organism. The role of DFFB in apoptosis highlights its importance in normal cell turnover and immune system regulation.

Pathways

DFFB is an essential component of the apoptotic signaling pathway. It aligns with the intrinsic mitochondrial pathway which involves cytochrome c release and subsequent apoptosome formation. Proteins such as caspase-3 activate DFFB by cleaving DFFA facilitating its nuclease function. The careful balance of these pathways and proteins ensures orderly cell death preventing unwanted inflammatory responses or pathological conditions.

Associated diseases and disorders

DFFB has connections to cancer and neurodegenerative disorders. Dysregulation of its apoptotic function can lead to unchecked cell proliferation contributing to tumorigenesis. Furthermore abnormal DFFB activity is linked to conditions such as Alzheimer's disease where excessive or inappropriate apoptosis occurs. The interplay between DFFB and proteins such as caspase-3 often becomes critical in these disorders highlighting the complex role of apoptosis in both development and pathology.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • SDS-PAGE - Recombinant Human DFFB/CAD protein (ab158274), expandable thumbnail

    SDS-PAGE - Recombinant Human DFFB/CAD protein (ab158274)

    ab158274 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com