Recombinant Human DFFB/CAD protein is a Human Full Length protein, in the 1 to 338 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Wheat germ
GST tag N-Terminus
ELISA, WB
No
M L Q K P K S V K L R A L R S P R K F G V A G R S C Q E V L R K G C L R F Q L P E R G S R L C L Y E D G T E L T E D Y F P S V P D N A E L V L L T L G Q A W Q G Y V S D I R R F L S A F H E P Q V G L I Q A A Q Q L L C D E Q A P Q R Q R L L A D L L H N V S Q N I A A E T R A E D P P W F E G L E S R F Q S K S G Y L R Y S C E S R I R S Y L R E V S S Y P S T V G A E A Q E E F L R V L G S M C Q R L R S M Q Y N G S Y F D R G A K G G S R L C T P E G W F S C Q G P F D M D S C L S R H S I N P Y S N R E S R I L F S T W N L D H I I E K K R T I I P T L V E A I K E Q D G R E V D W E Y F Y G L L F T S E N L K L V H I V C H K K T T H K L N C D P S R I Y K P Q T R L K R K Q P V R K R Q
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Select an associated product type
Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology.
CAD, DFF2, DFF40, DFF40, DFF2, CAD, DFFB, DNA fragmentation factor subunit beta, Caspase-activated deoxyribonuclease, Caspase-activated nuclease, DNA fragmentation factor 40 kDa subunit, CAD, Caspase-activated DNase, CPAN, DFF-40
Recombinant Human DFFB/CAD protein is a Human Full Length protein, in the 1 to 338 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Wheat germ
GST tag N-Terminus
ELISA, WB
No
No
Human
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
M L Q K P K S V K L R A L R S P R K F G V A G R S C Q E V L R K G C L R F Q L P E R G S R L C L Y E D G T E L T E D Y F P S V P D N A E L V L L T L G Q A W Q G Y V S D I R R F L S A F H E P Q V G L I Q A A Q Q L L C D E Q A P Q R Q R L L A D L L H N V S Q N I A A E T R A E D P P W F E G L E S R F Q S K S G Y L R Y S C E S R I R S Y L R E V S S Y P S T V G A E A Q E E F L R V L G S M C Q R L R S M Q Y N G S Y F D R G A K G G S R L C T P E G W F S C Q G P F D M D S C L S R H S I N P Y S N R E S R I L F S T W N L D H I I E K K R T I I P T L V E A I K E Q D G R E V D W E Y F Y G L L F T S E N L K L V H I V C H K K T T H K L N C D P S R I Y K P Q T R L K R K Q P V R K R Q
Full Length
1 to 338
Recombinant
GST tag N-Terminus
Liquid
Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology.
Nucleus
Dry Ice
-80°C
-80°C
Upon delivery aliquot
Avoid freeze / thaw cycle
This product was previously labelled as Recombinant Human DFFB protein.
This supplementary information is collated from multiple sources and compiled automatically.
DFFB also known as DNA fragmentation factor subunit beta or CAD is a protein with a molecular mass of approximately 40 kDa. It plays an important role in the apoptotic process by acting as a nuclease responsible for cleaving DNA into oligonucleosomal fragments. DFFB is found in various tissues expressing mainly in cells undergoing apoptosis. The interaction with its inhibitor DFFA prevents its activation until the apoptotic signal triggers the pathway for its release and activation resulting in DNA fragmentation.
DFFB is critical in the execution phase of apoptosis which is the programmed cell death essential to maintaining cellular homeostasis and development. It forms a complex with DFFA its chaperone and inhibitor which regulates its nuclease activity. Apoptosis requires controlled DNA degradation activity which DFFB provides ensuring that cells dismantle DNA in a process safe for the organism. The role of DFFB in apoptosis highlights its importance in normal cell turnover and immune system regulation.
DFFB is an essential component of the apoptotic signaling pathway. It aligns with the intrinsic mitochondrial pathway which involves cytochrome c release and subsequent apoptosome formation. Proteins such as caspase-3 activate DFFB by cleaving DFFA facilitating its nuclease function. The careful balance of these pathways and proteins ensures orderly cell death preventing unwanted inflammatory responses or pathological conditions.
DFFB has connections to cancer and neurodegenerative disorders. Dysregulation of its apoptotic function can lead to unchecked cell proliferation contributing to tumorigenesis. Furthermore abnormal DFFB activity is linked to conditions such as Alzheimer's disease where excessive or inappropriate apoptosis occurs. The interplay between DFFB and proteins such as caspase-3 often becomes critical in these disorders highlighting the complex role of apoptosis in both development and pathology.
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ab158274 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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