Recombinant Human DGAT2 protein is a Human Fragment protein, in the 289 to 388 aa range, expressed in Wheat germ and suitable for ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
I F E E G S W G R W V Q K K F Q K Y I G F A P C I F H G R G L F S S D T W G L V P Y S K P I T T V V G E P I T I P K L E H P T Q Q D I D L Y H T M Y M E A L V K L F D K H K T K F G L P E T E V L E V N
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Essential acyltransferase that catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for synthesis and storage of intracellular triglycerides (PubMed:27184406). Probably plays a central role in cytosolic lipid accumulation. In liver, is primarily responsible for incorporating endogenously synthesized fatty acids into triglycerides (By similarity). Functions also as an acyl-CoA retinol acyltransferase (ARAT) (By similarity). Also able to use 1-monoalkylglycerol (1-MAkG) as an acyl acceptor for the synthesis of monoalkyl-monoacylglycerol (MAMAG) (PubMed:28420705).
Alternative names
HMFN1045, UNQ738/PRO1433, DGAT2, Diacylglycerol O-acyltransferase 2, Acyl-CoA retinol O-fatty-acyltransferase, Diglyceride acyltransferase 2, ARAT, Retinol O-fatty-acyltransferase
Recommended products
Recombinant Human DGAT2 protein is a Human Fragment protein, in the 289 to 388 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- Q96PD7-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- I F E E G S W G R W V Q K K F Q K Y I G F A P C I F H G R G L F S S D T W G L V P Y S K P I T T V V G E P I T I P K L E H P T Q Q D I D L Y H T M Y M E A L V K L F D K H K T K F G L P E T E V L E V N
- Accession
- Q96PD7
- Protein length
- Fragment
- Amino acids
- 289 to 388
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Essential acyltransferase that catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for synthesis and storage of intracellular triglycerides (PubMed:27184406). Probably plays a central role in cytosolic lipid accumulation. In liver, is primarily responsible for incorporating endogenously synthesized fatty acids into triglycerides (By similarity). Functions also as an acyl-CoA retinol acyltransferase (ARAT) (By similarity). Also able to use 1-monoalkylglycerol (1-MAkG) as an acyl acceptor for the synthesis of monoalkyl-monoacylglycerol (MAMAG) (PubMed:28420705).
- Sequence similarities
Belongs to the diacylglycerol acyltransferase family.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The diacylglycerol O-acyltransferase 2 commonly referred to as DGAT2 plays a mechanical role in triglyceride biosynthesis by catalyzing the final step of triacylglycerol formation from diacylglycerol and acyl-CoA. This protein also functions under the name acyl-CoA:diacylglycerol acyltransferase 2. DGAT2 has a molecular weight of approximately 40-45 kDa. The enzyme is mainly expressed in tissues like the liver adipose tissue and intestines where lipid metabolism is an important function.
- Biological function summary
DGAT2 significantly contributes to energy storage and lipid homeostasis in cells. The protein is not usually known to be part of a larger macromolecular complex but it works in association with lipid droplets where the fats are stored. The enzyme's activity significantly impacts the regulation of intracellular triglyceride levels making it important for maintaining energy balance within the cell and the entire organism.
- Pathways
The role of DGAT2 is mainly tied to lipid metabolism particularly the triglyceride synthesis pathway. It directly assists in converting excess carbohydrates and other substrates into triglycerides for energy storage. DGAT2 works closely with proteins like DGAT1 another isoform involved in triacylglycerol synthesis and both contribute to maintaining adequate lipid storage and energy regulation within the body.
- Associated diseases and disorders
DGAT2 has implications in metabolic diseases such as obesity and non-alcoholic fatty liver disease (NAFLD). An overexpression or increased activity of DGAT2 can lead to excessive lipid formation and storage contributing to these conditions. It shows interaction with proteins like perilipin which are involved in lipid droplet formation influencing pathological fat accumulation in tissues. Low levels or activity of DGAT2 have also been investigated concerning insulin resistance further linking it to metabolic syndrome-related disorders.
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1 product image
SDS-PAGE - Recombinant Human DGAT2 protein (ab153553)
ab153553 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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