Recombinant human DGKA protein (Active) is a Human Full Length protein, in the 1 to 735 aa range, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, FuncS.
Images
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M A K E R G L I S P S D F A Q L Q K Y M E Y S T K K V S D V L K L F E D G E M A K Y V Q G D A I G Y E G F Q Q F L K I Y L E V D N V P R H L S L A L F Q S F E T G H C L N E T N V T K D V V C L N D V S C Y F S L L E G G R P E D K L E F T F K L Y D T D R N G I L D S S E V D K I I L Q M M R V A E Y L D W D V S E L R P I L Q E M M K E I D Y D G S G S V S Q A E W V R A G A T T V P L L V L L G L E M T L K D D G Q H M W R P K R F P R P V Y C N L C E S S I G L G K Q G L S C N L C K Y T V H D Q C A M K A L P C E V S T Y A K S R K D I G V Q S H V W V R G G C E S G R C D R C Q K K I R I Y H S L T G L H C V W C H L E I H D D C L Q A V G H E C D C G L L R D H I L P P S S I Y P S V L A S G P D R K N S K T S Q K T M D D L N L S T S E A L R I D P V P N T H P L L V F V N P K S G G K Q G Q R V L W K F Q Y I L N P R Q V F N L L K D G P E I G L R L F K D V P D S R I L V C G G D G T V G W I L E T I D K A N L P V L P P V A V L P L G T G N D L A R C L R W G G G Y E G Q N L A K I L K D L E M S K V V H M D R W S V E V I P Q Q T E E K S D P V P F Q I I N N Y F S I G V D A S I A H R F H I M R E K Y P E K F N S R M K N K L W Y F E F A T S E S I F S T C K K L E E S L T V E I C G K P L D L S N L S L E G I A V L N I P S M H G G S N L W G D T R R P H G D I Y G I N Q A L G A T A K V I T D P D I L K T C V P D L S D K R L E V V G L E G A I E M G Q I Y T K L K N A G R R L A K C S E I T F H T T K T L P M Q I D G E P W M Q T P C T I K I T H K N Q M P M L M G P P P R S T N F F G F L S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed:15544348, PubMed:2175712). Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (PubMed:15544348, PubMed:2175712). Also plays an important role in the biosynthesis of complex lipids (Probable). Can also phosphorylate 1-alkyl-2-acylglycerol in vitro as efficiently as diacylglycerol provided it contains an arachidonoyl group (PubMed:15544348). Also involved in the production of alkyl-lysophosphatidic acid, another bioactive lipid, through the phosphorylation of 1-alkyl-2-acetyl glycerol (PubMed:22627129).
Alternative names
DAGK, DAGK1, DGKA, Diacylglycerol kinase alpha, DAG kinase alpha, 80 kDa diacylglycerol kinase, Diglyceride kinase alpha, DGK-alpha
Recommended products
Recombinant human DGKA protein (Active) is a Human Full Length protein, in the 1 to 735 aa range, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- The specific activity of ab268441 was 29.1 nmol/min/mg in a kinase assay using Dilauroyl-sn-glycerol as substrate.
- Accession
- P23743-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7
Preservative: 1.02% Imidazole
Constituents: 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 0.82% Sodium phosphate, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF
Sequence info
Amino acid sequence
- M A K E R G L I S P S D F A Q L Q K Y M E Y S T K K V S D V L K L F E D G E M A K Y V Q G D A I G Y E G F Q Q F L K I Y L E V D N V P R H L S L A L F Q S F E T G H C L N E T N V T K D V V C L N D V S C Y F S L L E G G R P E D K L E F T F K L Y D T D R N G I L D S S E V D K I I L Q M M R V A E Y L D W D V S E L R P I L Q E M M K E I D Y D G S G S V S Q A E W V R A G A T T V P L L V L L G L E M T L K D D G Q H M W R P K R F P R P V Y C N L C E S S I G L G K Q G L S C N L C K Y T V H D Q C A M K A L P C E V S T Y A K S R K D I G V Q S H V W V R G G C E S G R C D R C Q K K I R I Y H S L T G L H C V W C H L E I H D D C L Q A V G H E C D C G L L R D H I L P P S S I Y P S V L A S G P D R K N S K T S Q K T M D D L N L S T S E A L R I D P V P N T H P L L V F V N P K S G G K Q G Q R V L W K F Q Y I L N P R Q V F N L L K D G P E I G L R L F K D V P D S R I L V C G G D G T V G W I L E T I D K A N L P V L P P V A V L P L G T G N D L A R C L R W G G G Y E G Q N L A K I L K D L E M S K V V H M D R W S V E V I P Q Q T E E K S D P V P F Q I I N N Y F S I G V D A S I A H R F H I M R E K Y P E K F N S R M K N K L W Y F E F A T S E S I F S T C K K L E E S L T V E I C G K P L D L S N L S L E G I A V L N I P S M H G G S N L W G D T R R P H G D I Y G I N Q A L G A T A K V I T D P D I L K T C V P D L S D K R L E V V G L E G A I E M G Q I Y T K L K N A G R R L A K C S E I T F H T T K T L P M Q I D G E P W M Q T P C T I K I T H K N Q M P M L M G P P P R S T N F F G F L S
- Accession
- P23743
- Protein length
- Full Length
- Amino acids
- 1 to 735
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed:15544348, PubMed:2175712). Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (PubMed:15544348, PubMed:2175712). Also plays an important role in the biosynthesis of complex lipids (Probable). Can also phosphorylate 1-alkyl-2-acylglycerol in vitro as efficiently as diacylglycerol provided it contains an arachidonoyl group (PubMed:15544348). Also involved in the production of alkyl-lysophosphatidic acid, another bioactive lipid, through the phosphorylation of 1-alkyl-2-acetyl glycerol (PubMed:22627129).
- Sequence similarities
Belongs to the eukaryotic diacylglycerol kinase family.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
DGKA also known as diacylglycerol kinase alpha plays an important role in the conversion of diacylglycerol (DAG) to phosphatidic acid (PA) by transferring a phosphate group from ATP. This enzymatic process regulates levels of DAG and PA which are critical molecules for signaling processes. DGKA has a molecular weight of approximately 80 kDa. Expression of DGKA occurs in various tissues with notable presence in the brain and immune system highlighting its significance in neurological and immunological functions.
- Biological function summary
DGKA modulates multiple signaling pathways by controlling the availability of DAG and PA essential intermediates in intracellular signaling. It operates as part of a complex that interacts with various phosphorylated proteins to affect downstream signaling. The enzyme influences cellular processes such as proliferation differentiation and apoptosis by modulating DAG-dependent signaling pathways.
- Pathways
DGKA integrates into significant signaling cascades including the PI3K/Akt and MAPK pathways. In the PI3K/Akt pathway DGKA modulates the activity of proteins like PKC through its DAG-regulating function. Alternatively in the MAPK pathway it influences protein phosphorylation through effects on upstream kinases. This regulation highlights the enzyme's role in critical cell fate decisions.
- Associated diseases and disorders
DGKA has associations with certain cancers and immune-related diseases. Aberrant DGKA activity links to oncogenic processes influencing tumor growth and progression via its impact on the PI3K/Akt pathway. In immune disorders dysregulated DGKA activity connects to excessive inflammation affecting proteins such as PKC that are involved in immune responses. Understanding DGKA's role in these diseases could drive new therapeutic strategies.
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2 product images
Functional Studies - Recombinant human DGKA protein (Active) (ab268441)
The specific activity of ab268441 was 29.1 nmol/min/mg in a kinase assay using Dilauroyl-sn-glycerol as substrate.
SDS-PAGE - Recombinant human DGKA protein (Active) (ab268441)
SDS-PAGE analysis of ab268441.
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