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AB268445

Recombinant human DGKZ/DGK-zeta protein (Active)

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Recombinant human DGKZ/DGK-zeta protein (Active) is a Human Full Length protein, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS.

View Alternative Names

DAGK6, DGKZ, Diacylglycerol kinase zeta, DAG kinase zeta, Diglyceride kinase zeta, DGK-zeta

2 Images
Functional Studies - Recombinant human DGKZ/DGK-zeta protein (Active) (AB268445)
  • FuncS

Supplier Data

Functional Studies - Recombinant human DGKZ/DGK-zeta protein (Active) (AB268445)

The specific activity of ab268445 was 18.2 nmol/min/mg in a kinase assay using Dilauroyl-sn-glycerol as substrate.

SDS-PAGE - Recombinant human DGKZ/DGK-zeta protein (Active) (AB268445)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human DGKZ/DGK-zeta protein (Active) (AB268445)

SDS-PAGE analysis of ab268445.

Key facts

Purity

>90% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

The specific activity of ab268445 was 18.2 nmol/min/mg in a kinase assay using Dilauroyl-sn-glycerol as substrate.

Accession

Q13574

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q13574","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DGKZ also known as DGK-zeta or DGKζ is an enzyme that belongs to the diacylglycerol kinase family and plays an important role in lipid signaling pathways. The 'zeta' isoform of DGK sometimes noted in scientific literature as DGK ART and DGK MEXICO catalyzes the conversion of diacylglycerol (DAG) to phosphatidic acid (PA) by ATP-dependent phosphorylation impacting signal transduction. DGKZ has a molecular mass of approximately 115 kDa. It shows expression in various tissues including brain heart and skeletal muscle with higher expression levels typically seen in the nervous system where it affects neurotransmitter signaling.
Biological function summary

DGKZ influences cellular processes such as growth differentiation and apoptosis by regulating DAG and PA levels. It forms part of a complex network of proteins involved in intracellular signaling cascades exerting effects on protein kinase C (PKC) activity. The regulatory function of DGKZ within cellular activity highlights its role in maintaining normal cellular homeostasis and responding to extracellular signals. Its enzymatic activity influences DAG-mediated pathways which are important for the activation and deactivation of downstream signaling proteins.

Pathways

DGKZ plays a critical role within the signaling pathways such as the phosphoinositide signaling and mTOR pathways. It serves as an important modulator of lipid signaling within the diacylglycerol- and phosphatidic acid-mediated signaling pathways impacting protein interactions and signaling mechanisms. Other proteins related through these pathways include PKC and mTOR with which DGKZ interacts to modulate functions such as cell proliferation and survival. The enzymatic conversion of DAG to PA by DGKZ actively regulates the availability of these lipid signaling molecules and therefore modulates their downstream effects.

DGKZ has associations with neurological disorders and cancer. Abnormal regulation or expression of DGKZ contributes to pathologies such as Alzheimer's disease where impaired lipid signaling affects neuronal function and survival. Additionally its dysregulation may promote cancer progression through aberrant cell signaling pathways. DGKZ interacts with proteins like PKC which play a role in these diseases making it a potential therapeutic target. Understanding its function and interactions helps offer insights into the development of targeted therapies for these conditions.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed : 15544348, PubMed : 18004883, PubMed : 19744926, PubMed : 22108654, PubMed : 22627129, PubMed : 23949095, PubMed : 9159104). Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (PubMed : 15544348, PubMed : 18004883, PubMed : 19744926, PubMed : 22108654, PubMed : 22627129, PubMed : 23949095, PubMed : 9159104). Also plays an important role in the biosynthesis of complex lipids (Probable). Does not exhibit an acyl chain-dependent substrate specificity among diacylglycerol species (PubMed : 19744926, PubMed : 22108654, PubMed : 9159104). Can also phosphorylate 1-alkyl-2-acylglycerol in vitro but less efficiently and with a preference for alkylacylglycerols containing an arachidonoyl group (PubMed : 15544348, PubMed : 19744926, PubMed : 22627129). The biological processes it is involved in include T cell activation since it negatively regulates T-cell receptor signaling which is in part mediated by diacylglycerol (By similarity). By generating phosphatidic acid, stimulates PIP5KIA activity which regulates actin polymerization (PubMed : 15157668). Through the same mechanism could also positively regulate insulin-induced translocation of SLC2A4 to the cell membrane (By similarity).. Isoform 1. Regulates RASGRP1 activity.. Isoform 2. Does not regulate RASGRP1 activity.

Sequence similarities

Belongs to the eukaryotic diacylglycerol kinase family.

Post-translational modifications

Phosphorylation of the MARCKS homology domain by PKC reduces nuclear accumulation of DGK-zeta.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed : 15544348, PubMed : 18004883, PubMed : 19744926, PubMed : 22108654, PubMed : 22627129, PubMed : 23949095, PubMed : 9159104). Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (PubMed : 15544348, PubMed : 18004883, PubMed : 19744926, PubMed : 22108654, PubMed : 22627129, PubMed : 23949095, PubMed : 9159104). Also plays an important role in the biosynthesis of complex lipids (Probable). Does not exhibit an acyl chain-dependent substrate specificity among diacylglycerol species (PubMed : 19744926, PubMed : 22108654, PubMed : 9159104). Can also phosphorylate 1-alkyl-2-acylglycerol in vitro but less efficiently and with a preference for alkylacylglycerols containing an arachidonoyl group (PubMed : 15544348, PubMed : 19744926, PubMed : 22627129). The biological processes it is involved in include T cell activation since it negatively regulates T-cell receptor signaling which is in part mediated by diacylglycerol (By similarity). By generating phosphatidic acid, stimulates PIP5KIA activity which regulates actin polymerization (PubMed : 15157668). Through the same mechanism could also positively regulate insulin-induced translocation of SLC2A4 to the cell membrane (By similarity).. Isoform 1. Regulates RASGRP1 activity.. Isoform 2. Does not regulate RASGRP1 activity.
See full target information DGKZ
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