Recombinant Human DHCR7 protein is a Human Full Length protein, in the 1 to 475 aa range, expressed in Wheat germ and suitable for ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
M A A K L Q P N I P K A K S L D G V T N D R T A S Q G Q W G R A W E V D W F S L A S V I F L L L F A P F I V Y Y F I M A C D Q Y S C A L T G P V V D I V T G H A R L S D I W A K T P P I T R K A A Q L Y T L W V T F Q V L L Y T S L P D F C H K F L P G Y V G G I Q E G A V T P A G V V N K Y Q I N G L Q A W L L T H L L W F A N A H L L S W F S P T I I F D N W I P L L W C A N I L G Y A V S T F A M V K G Y F F P T S A R D C K F T G N F F Y N Y M M G I E F N P R I G K W F D F K L F F N G R P G I V A W T L I N L S F A A K Q R E L H S H V T N A M V L V N V L Q A I Y V I D F F W N E T W Y L K T I D I C H D H F G W Y L G W G D C V W L P Y L Y T L Q G L Y L V Y H P V Q L S T P H A V G V L L L G L V G Y Y I F R V A N H Q K D L F R R T D G R C L I W G R K P K V I E C S Y T S A D G Q R H H S K L L V S G F W G V A R H F N Y V G D L M G S L A Y C L A C G G G H L L P Y F Y I I Y M A I L L T H R C L R D E H R C A S K Y G R D W E R Y T A A V P Y R L L P G I F
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Catalyzes the last step of the cholesterol synthesis pathway, which transforms cholesta-5,7-dien-3beta-ol (7-dehydrocholesterol,7-DHC) into cholesterol by reducing the C7-C8 double bond of its sterol core (PubMed:25637936, PubMed:38297129, PubMed:38297130, PubMed:9465114, PubMed:9634533). Can also metabolize cholesta-5,7,24-trien-3beta-ol (7-dehydrodemosterol, 7-DHD) to desmosterol, which is then metabolized by the Delta(24)-sterol reductase (DHCR24) to cholesterol (By similarity). Modulates ferroptosis (a form of regulated cell death driven by iron-dependent lipid peroxidation) through the metabolic breakdown of the anti-ferroptotic metabolites 7-DHC and 7-DHD which, when accumulated, divert the propagation of peroxyl radical-mediated damage from phospholipid components to its sterol core, protecting plasma and mitochondrial membranes from phospholipid autoxidation (PubMed:38297129, PubMed:38297130).
Alternative names
D7SR, DHCR7, 7-dehydrocholesterol reductase, 7-DHC reductase, Delta7-sterol reductase, Sterol Delta(7)-reductase, Sterol reductase SR-2
Recommended products
Recombinant Human DHCR7 protein is a Human Full Length protein, in the 1 to 475 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- Q9UBM7-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M A A K L Q P N I P K A K S L D G V T N D R T A S Q G Q W G R A W E V D W F S L A S V I F L L L F A P F I V Y Y F I M A C D Q Y S C A L T G P V V D I V T G H A R L S D I W A K T P P I T R K A A Q L Y T L W V T F Q V L L Y T S L P D F C H K F L P G Y V G G I Q E G A V T P A G V V N K Y Q I N G L Q A W L L T H L L W F A N A H L L S W F S P T I I F D N W I P L L W C A N I L G Y A V S T F A M V K G Y F F P T S A R D C K F T G N F F Y N Y M M G I E F N P R I G K W F D F K L F F N G R P G I V A W T L I N L S F A A K Q R E L H S H V T N A M V L V N V L Q A I Y V I D F F W N E T W Y L K T I D I C H D H F G W Y L G W G D C V W L P Y L Y T L Q G L Y L V Y H P V Q L S T P H A V G V L L L G L V G Y Y I F R V A N H Q K D L F R R T D G R C L I W G R K P K V I E C S Y T S A D G Q R H H S K L L V S G F W G V A R H F N Y V G D L M G S L A Y C L A C G G G H L L P Y F Y I I Y M A I L L T H R C L R D E H R C A S K Y G R D W E R Y T A A V P Y R L L P G I F
- Accession
- Q9UBM7
- Protein length
- Full Length
- Amino acids
- 1 to 475
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Catalyzes the last step of the cholesterol synthesis pathway, which transforms cholesta-5,7-dien-3beta-ol (7-dehydrocholesterol,7-DHC) into cholesterol by reducing the C7-C8 double bond of its sterol core (PubMed:25637936, PubMed:38297129, PubMed:38297130, PubMed:9465114, PubMed:9634533). Can also metabolize cholesta-5,7,24-trien-3beta-ol (7-dehydrodemosterol, 7-DHD) to desmosterol, which is then metabolized by the Delta(24)-sterol reductase (DHCR24) to cholesterol (By similarity). Modulates ferroptosis (a form of regulated cell death driven by iron-dependent lipid peroxidation) through the metabolic breakdown of the anti-ferroptotic metabolites 7-DHC and 7-DHD which, when accumulated, divert the propagation of peroxyl radical-mediated damage from phospholipid components to its sterol core, protecting plasma and mitochondrial membranes from phospholipid autoxidation (PubMed:38297129, PubMed:38297130).
- Sequence similarities
Belongs to the ERG4/ERG24 family.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
7-dehydrocholesterol reductase (DHCR7) is an enzyme that plays an important role in cholesterol biosynthesis. It catalyzes the conversion of 7-dehydrocholesterol (7-DHC) to cholesterol in the final step of the process. DHCR7 is also known by the name sterol Δ7-reductase. The enzyme has a molecular mass of approximately 54 kDa. Expression of DHCR7 is prominent in tissues that require active cholesterol synthesis such as the liver adrenal glands and brain.
- Biological function summary
DHCR7 activity ensures the maintenance of cholesterol homeostasis in cells. The enzyme operates independently and does not form part of a larger complex focusing directly on the reduction of the double bond in the C7-8 position of 7-DHC to yield cholesterol. By regulating cholesterol levels DHCR7 supports membrane fluidity and integrity contributing to the proper function of cellular structures.
- Pathways
DHCR7 is an important component of the cholesterol biosynthesis pathway which is part of the larger sterol metabolic pathway. Its function is linked with other enzymes such as 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR) highlighting their roles in sequential steps that convert squalene into cholesterol. These interactions highlight the interdependence of DHCR7 with other sterol-processing proteins influencing both cholesterol production and regulation within the body.
- Associated diseases and disorders
DHCR7 mutations can cause conditions such as Smith-Lemli-Opitz syndrome a congenital disorder characterized by multiple physical and developmental abnormalities. This condition connects DHCR7 to defective cholesterol metabolism and accumulation of toxic 7-DHC levels. Additionally the involvement of DHCR7 in cholesterol homeostasis ties it with potential dyslipidemias where abnormal lipid levels may influence cardiovascular health. Changes in DHCR7 function can lead to disruptions in normal cholesterol synthesis impacting related proteins and enzymatic pathways.
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1 product image
SDS-PAGE - Recombinant Human DHCR7 protein (ab158282)
ab158282 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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