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AB128451

Recombinant Human DHODH protein

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(1 Publication)

Recombinant Human DHODH protein is a Human Fragment protein, in the 31 to 395 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

DHOdehase, Dihydroorotate oxidase, DHODH

1 Images
SDS-PAGE - Recombinant Human DHODH protein (AB128451)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human DHODH protein (AB128451)

15% SDS-PAGE showing ab128451 at approximately 42.3 kDa (3µg).

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Q02127

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Not currently tested for endotoxin levels
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSHMTGDERFYAEHLMPTLQGLLDPESAHRLAVRFTSLGLLPRARFQDSDMLEVRVLGHKFRNPVGIAAGFDKHGEAVDGLYKMGFGFVEIGSVTPKPQEGNPRPRVFRLPEDQAVINRYGFNSHGLSVVEHRLRARQQKQAKLTEDGLPLGVNLGKNKTSVDAAEDYAEGVRVLGPLADYLVVNVSSPNTAGLRSLQGKAELRRLLTKVLQERDGLRRVHRPAVLVKIAPDLTSQDKEDIASVVKELGIDGLIVTNTTVSRPAGLQGALRSETGGLSGKPLRDLSTQTIREMYALTQGRVPIIGVGGVSSGQDALEKIRAGASLVQLYTALTFWGPPVVGKVKRELEALLKEQGFGGVTDAIGADHRR","proteinLength":"Fragment","predictedMolecularWeight":"42.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":395,"aminoAcidStart":31,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q02127","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Dihydroorotate dehydrogenase (DHODH) also known as URA1 is an enzyme weighing approximately 43 kDa. It plays a critical role in the de novo pyrimidine biosynthesis pathway. DHODH is mainly expressed in the inner mitochondrial membrane where it catalyzes the conversion of dihydroorotate to orotate. This reaction involves ubiquinone as an electron acceptor making DHODH an important player in cellular respiration. Its functionality is essential for the synthesis of nucleotides particularly in rapidly proliferating cells.
Biological function summary

Dihydroorotate dehydrogenase is involved in establishing the pyrimidine pool necessary for DNA and RNA synthesis. It is a single-component enzyme but its activity supports the function of other enzymes in the pathway such as carbamoyl-phosphate synthetase. The proper regulation of DHODH is integral to cellular proliferation and immune responses. Cells that synthesize nucleotides at high rates such as immune cells and cancer cells heavily depend on its activity.

Pathways

Dihydroorotate dehydrogenase is an important player in the pyrimidine biosynthesis pathway and links to the mitochondrial electron transport chain. Its interaction with ubiquinone underlines its role in energy production connecting it to respiratory complexes. DHODH works alongside proteins such as orotate phosphoribosyltransferase which further transforms orotate to orotidine-5'-monophosphate in pyrimidine biosynthesis. These pathways are vital for maintaining the balance of nucleotide levels impacting processes like DNA replication.

Dihydroorotate dehydrogenase is relevant to autoimmune diseases like rheumatoid arthritis and cancers. Inhibitors such as leflunomide and brequinar target DHODH to disrupt the proliferation of rapidly dividing cells. Leflunomide which is metabolized to teriflunomide reduces immune cell turnover in rheumatoid arthritis illustrating DHODH's therapeutic importance. Similarly it is a target in cancer treatment where its inhibition can slow down tumor growth interfacing with other metabolic regulators active in malignancies.
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Specifications

Form

Liquid

Additional notes

ab128451 is purified using conventional chromatography techniques (anion exchange and gel filtration)

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General info

Function

Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor. Required for UMP biosynthesis via de novo pathway.

Sequence similarities

Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.

Post-translational modifications

The uncleaved transit peptide is required for mitochondrial targeting and proper membrane integration.

Subcellular localisation

Mitochondrion inner membrane

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Product protocols

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Target data

Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor. Required for UMP biosynthesis via de novo pathway.
See full target information DHODH
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Aging 11:8433-8462 PubMed31560653

2019

Brain pyrimidine nucleotide synthesis and Alzheimer disease.

Applications

Unspecified application

Species

Unspecified reactive species

Alba Pesini,Eldris Iglesias,M Pilar Bayona-Bafaluy,Nuria Garrido-Pérez,Patricia Meade,Paula Gaudó,Irene Jiménez-Salvador,Pol Andrés-Benito,Julio Montoya,Isidro Ferrer,Pedro Pesini,Eduardo Ruiz-Pesini
View all publications
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Complementary Products

Primary Antibodies

AB174288

Anti-DHODH antibody [EPR11814]

RabMAb|Recombinant|20ul selling size

Western blot - Anti-DHODH antibody [EPR11814] (AB174288)

  • 0
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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
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Primary Antibodies

AB236868

Anti-SCD1 antibody [EPR21963]

KO Validated|RabMAb|Recombinant|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-SCD1 antibody [EPR21963] (AB236868)

  • 0
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Product promise

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