JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB87755

Recombinant human Dihydrofolate reductase (DHFR) protein (His tag N-Terminus)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant human Dihydrofolate reductase (DHFR) protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 187 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, FuncS.

View Alternative Names

Dihydrofolate reductase, DHFR

1 Images
SDS-PAGE - Recombinant human Dihydrofolate reductase (DHFR) protein (His tag N-Terminus) (AB87755)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human Dihydrofolate reductase (DHFR) protein (His tag N-Terminus) (AB87755)

ab87755 at 3 ug visualised by SDS-PAGE. Observed band size is approximately 27 kDa.

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Specific activity is > 2000pmol/min/μg, and is defined as the amount of enzyme that converts 1.0pmole of dihydrofolic acid to tetrahydrofolic acid per minute at pH 6.5 at 25°C.

Accession

P00374

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 30% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0308% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

style
left-column

Complementary Products

Primary Antibodies

AB124814

Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] (AB124814)

  • 4
  • 1
Assay Kits

AB239705

Dihydrofolate Reductase Assay Kit (Colorimetric)

Functional Studies - Dihydrofolate Reductase Assay Kit (Colorimetric) (AB239705)

  • 0
  • 0
Primary Antibodies

AB31940

Anti-TBR1 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR1 antibody (AB31940)

  • 4
  • 34
Primary Antibodies

AB32454

Anti-MAP2 antibody - Neuronal Marker

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody - Neuronal Marker (AB32454)

  • 5
  • 35
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMVGSLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND","proteinLength":"Full Length","predictedMolecularWeight":"23.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":187,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P00374","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
style
left-column
style
left-column

Specifications

Form

Liquid

Additional notes

purified by using conventional chromatography techniques.

style
left-column

General info

Function

Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFR2.

Sequence similarities

Belongs to the dihydrofolate reductase family.

Subcellular localisation

Mitochondrion

style
left-column

Product protocols

style
left-column

Target data

Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFR2.
See full target information Dihydrofolate reductase
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com