JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB130040

Recombinant Human DIMT1L protein

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human DIMT1L protein is a Human Full Length protein, in the 1 to 313 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

DIMT1L, HUSSY-05, DIMT1, Dimethyladenosine transferase, 18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase, 18S rRNA dimethylase, DIM1 dimethyladenosine transferase 1 homolog, DIM1 dimethyladenosine transferase 1-like

1 Images
SDS-PAGE - Recombinant Human DIMT1L protein (AB130040)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human DIMT1L protein (AB130040)

3ug by SDS-PAGE under reducing conditions and visualized by coomassie blue stain.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Q9UNQ2

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 30% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.32% Tris HCl, 0.06% EDTA, 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMMPKVKSGAIGRRRGRQEQRRELKSAGGLMFNTGIGQHILKNPLIINSIIDKAALRPTDVVLEVGPGTGNMTVKLLEKAKKVVACELDPRLVAELHKRVQGTPVASKLQVLVGDVLKTDLPFFDTCVANLPYQISSPFVFKLLLHRPFFRCAILMFQREFALRLVAKPGDKLYCRLSINTQLLARVDHLMKVGKNNFRPPPKVESSVVRIEPKNPPPPINFQEWDGLVRITFVRKNKTLSAAFKSSAVQQLLEKNYRIHCSVHNIIIPEDFSIADKIQQILTSTGFSDKRARSMDIDDFIRLLHGFNAEGIHFS","proteinLength":"Full Length","predictedMolecularWeight":"37.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":313,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9UNQ2","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DIMT1L also known as DIMT1 or Nsun5 is a 58 kDa protein involved in ribosomal RNA methylation. It functions as a methyltransferase introducing methyl groups at specific adenine residues in the small subunit ribosomal RNA (rRNA). The protein shows expression mainly in tissues with high rates of protein synthesis such as liver kidney and pancreas reflecting its role in ribosome biogenesis. Its widespread expression in proliferative tissues highlights its importance for cellular growth and division.
Biological function summary

The protein DIMT1L plays an important role in assembling the 40S ribosomal subunit a part of the eukaryotic 80S ribosome. As a methyltransferase this protein methylates adenine residues in rRNA influencing the ribosome's structural and functional integrity. It usually functions as a monomer not requiring large complexes to exert its effects. This methyltransferase activity is important for correct ribosome maturation which in turn affects protein synthesis capacity within cells.

Pathways

DIMT1L activity is integral to ribosome biogenesis and rRNA processing pathways. The protein interacts with components of the ribonucleoprotein complex to facilitate the final maturation steps of the 40S subunit. It associates with proteins like Nop14 which participate in similar rRNA processing pathways ensuring the fidelity of ribosome assembly. Its role in these pathways highlights the importance of ribosomal integrity for effective translation and overall cellular function.

Research links DIMT1L to certain cancers and growth defects. Aberrations in its expression or function can lead to dysregulation in ribosome production contributing to tumorigenesis. The protein sometimes connects to pathways involving tumor suppressor proteins like p53 where disruption can accelerate cancer progression. Beyond cancer other proliferative disorders might arise from alterations in DIMT1L-mediated processes highlighting the need for further exploration into its mechanistic impacts on human health.
style
left-column

Specifications

Form

Liquid

Additional notes

ab130040 is purified using conventional chromatography techniques.

style
left-column

General info

Function

Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 18S rRNA in the 40S particle (PubMed : 25851604). Involved in the pre-rRNA processing steps leading to small-subunit rRNA production independently of its RNA-modifying catalytic activity (PubMed : 25851604). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed : 34516797).

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family.

Subcellular localisation

Nucleus

style
left-column

Product protocols

style
left-column

Target data

Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 18S rRNA in the 40S particle (PubMed : 25851604). Involved in the pre-rRNA processing steps leading to small-subunit rRNA production independently of its RNA-modifying catalytic activity (PubMed : 25851604). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed : 34516797).
See full target information DIMT1
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB92554

Anti-Cullin 4A/CUL-4A antibody [EPR3198]

RabMAb|Recombinant

Western blot - Anti-Cullin 4A/CUL-4A antibody [EPR3198] (AB92554)

  • 3
  • 1
Primary Antibodies

AB251661

Anti-UPF3B/RENT3B antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UPF3B/RENT3B antibody (AB251661)

  • 0
  • 0
Primary Antibodies

AB202064

Anti-PPCEL/PREPL antibody [EPR17751]

RabMAb|Recombinant

Western blot - Anti-PPCEL/PREPL antibody [EPR17751] (AB202064)

  • 0
  • 0
Primary Antibodies

AB220160

Anti-YTHDC2 antibody [EPR21820-49]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDC2 antibody [EPR21820-49] (AB220160)

  • 5
  • 4

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com