Recombinant Human DIPP protein
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Recombinant Human DIPP protein is a Human Full Length protein, in the 1 to 172 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.
View Alternative Names
DIPP, DIPP1, NUDT3, Diphosphoinositol polyphosphate phosphohydrolase 1, DIPP-1, Diadenosine hexaphosphate hydrolase, Endopolyphosphatase, Nucleoside diphosphate-linked moiety X motif 3, m7GpppN-mRNA hydrolase, m7GpppX diphosphatase, Ap6A hydrolase, Nudix motif 3
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant Human DIPP protein (AB103915)
15% SDS-PAGE analysis of ab103915 (3 μg).
Reactivity data
Product details
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
DIPP regulates the concentration of diadenosine polyphosphates which act as signaling molecules in cellular communication. These molecules control intracellular and extracellular nucleotide levels. DIPP sometimes forms part of a protein complex that modulates signal transduction pathways emphasizing its role in cellular homeostasis and signaling.
Pathways
DIPP integrates into the purinergic signaling pathway and it contributes to the regulation of energy metabolism. This protein interacts with other hydrolases and kinases influencing ATP degradation and synthesis. Through this interaction it plays a role with proteins like NTPDases highlighting its significance in nucleotide management.
Specifications
Form
Liquid
Additional notes
Purified using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA.
General info
Function
Cleaves a beta-phosphate from the diphosphate groups in PP-InsP5 (diphosphoinositol pentakisphosphate) and [PP]2-InsP4 (bisdiphosphoinositol tetrakisphosphate), suggesting that it may play a role in signal transduction (PubMed : 10585413, PubMed : 12370170, PubMed : 9822604). InsP6 (inositol hexakisphosphate) is not a substrate (PubMed : 9822604). Acts as a negative regulator of the ERK1/2 pathway (By similarity). Also able to catalyze the hydrolysis of dinucleoside oligophosphates, with diadenosine 5',5'''-P1,P6-hexaphosphate (Ap6A) and diadenosine 5',5'''- P1,P5-pentaphosphate (Ap5A) being the preferred substrates (PubMed : 10419486, PubMed : 12370170). The major reaction products are ADP and p4a from Ap6A and ADP and ATP from Ap5A (PubMed : 12370170). Also able to hydrolyze 5-phosphoribose 1-diphosphate (PubMed : 12370170). Acts as a decapping enzyme that modulates the stability of a subset of mRNAs implicated in cell motility (PubMed : 26932476). Hydrolyzes monomethylated capped RNA after both the alpha- and beta-phosphates generating m7GMP + ppRNA and m7GDP + pRNA (PubMed : 32727897). Can hydrolyze unmethylated capped RNAs (By similarity). Divalent cations zinc, magnesium and manganese determine its substrate specificity (PubMed : 34788624). Exhibits diphosphoinositol polyphosphate phosphohydrolase in the presence of magnesium ions, diadenosine hexaphosphate hydrolase activity in the presence of manganese ions and endopolyphosphatase activity in the presence of zinc ions (PubMed : 34788624). Plays an important role in limiting DNA damage and maintaining cell survival upon oxidative stress via its endopolyphosphatase activity (PubMed : 34788624).
Sequence similarities
Belongs to the Nudix hydrolase family. DIPP subfamily.
Target data
Complementary Products
Product promise
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