Recombinant Human DKK2 protein (denatured) is a Human Full Length protein, in the 34 to 259 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M K L N S I K S S L G G E T P G Q A A N R S A G M Y Q G L A F G G S K K G K N L G Q A Y P C S S D K E C E V G R Y C H S P H Q G S S A C M V C R R K K K R C H R D G M C C P S T R C N N G I C I P V T E S I L T P H I P A L D G T R H R D R N H G H Y S N H D L G W Q N L G R P H T K M S H I K G H E G D P C L R S S D C I E G F C C A R H F W T K I C K P V L H Q G E V C T K Q R K K G S H G L E I F Q R C D C A K G L S C K V W K D A T Y S S K A R L H V C Q K I
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (By similarity).
Alternative names
UNQ682/PRO1316, DKK2, Dickkopf-related protein 2, Dickkopf-2, Dkk-2, hDkk-2
Recommended products
Recombinant Human DKK2 protein (denatured) is a Human Full Length protein, in the 34 to 259 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q9UBU2-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M K L N S I K S S L G G E T P G Q A A N R S A G M Y Q G L A F G G S K K G K N L G Q A Y P C S S D K E C E V G R Y C H S P H Q G S S A C M V C R R K K K R C H R D G M C C P S T R C N N G I C I P V T E S I L T P H I P A L D G T R H R D R N H G H Y S N H D L G W Q N L G R P H T K M S H I K G H E G D P C L R S S D C I E G F C C A R H F W T K I C K P V L H Q G E V C T K Q R K K G S H G L E I F Q R C D C A K G L S C K V W K D A T Y S S K A R L H V C Q K I
- Accession
- Q9UBU2
- Protein length
- Full Length
- Predicted molecular weight
- 27.6 kDa
- Amino acids
- 34 to 259
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (By similarity).
- Sequence similarities
Belongs to the dickkopf family.
- Post-translational modifications
May be proteolytically processed by a furin-like protease.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
DKK2 also known as Dickkopf-related protein 2 is a member of the Dickkopf family of proteins that inhibit the Wnt signaling pathway. It has a mass of approximately 28 kDa. DKK2 is expressed in various tissues including the eye bone and the central nervous system. It acts by binding to LRP5/6 co-receptors preventing Wnt proteins from interacting with their target receptors which inhibits downstream signaling.
- Biological function summary
DKK2 functions to modulate the balance between cell proliferation and differentiation. It does not form a complex on its own but interacts with other proteins such as LRP5/6 and Kremen1 to exert its inhibitory effects. The regulation by DKK2 is essential in processes like embryonic development bone formation and maintenance of tissue homeostasis. Its overexpression or lack of expression can lead to varied outcomes depending on the biological context it is involved in.
- Pathways
DKK2 plays a critical role in the Wnt signaling pathway which is important for regulating gene expression cell behavior and developmental processes. The protein interacts specifically with LRP6 inhibiting the pathway and influencing downstream molecules like beta-catenin. Additionally DKK2 has ties with the BMP signaling pathway where it has a more modulatory role impacting bone morphogenetic proteins during bone and cartilage formation.
- Associated diseases and disorders
DKK2 has connections with cancer and osteoporosis. In cancer abnormal DKK2 expression can either suppress or promote tumor progression depending on the type of cancer and the tumor environment where its interaction with proteins like beta-catenin becomes significant. In osteoporosis DKK2's role in bone remodeling is highlighted influencing bone density and health. The connection with LRP5 also links DKK2 to this bone disease as LRP5 mutations have implications in bone mass regulation.
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1 product image
SDS-PAGE - Recombinant Human DKK2 protein (denatured) (ab131710)
15% SDS-PAGE showing ab131710 at approximately 27.6 kDa (3µg).
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