JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB151926

Recombinant Human DLK-1 protein

Be the first to review this product! Submit a review

|

(2 Publications)

Recombinant Human DLK-1 protein is a Human Fragment protein, in the 24 to 297 aa range, expressed in HEK 293 cells, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, HPLC.

View Alternative Names

DLK, DLK1, Protein delta homolog 1, DLK-1, pG2

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

HPLC, SDS-PAGE

applications

Biologically active

No

Accession

P80370

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute in PBS

Storage buffer

pH: 7.4 Constituents: 99% Phosphate Buffer, 0.88% Sodium chloride

storage-buffer

style
left-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB21682

Anti-DLK-1 antibody

Western blot - Anti-DLK-1 antibody (AB21682)

  • 4
  • 5
Proteins & Peptides

AB140810

Recombinant Human TET1 protein

SDS-PAGE - Recombinant Human TET1 protein (AB140810)

  • 0
  • 0
Assay Kits

AB270211

Human DLK1 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human DLK1 ELISA Kit (AB270211)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"AECFPACNPQNGFCEDDNVCRCQPGWQGPLCDQCVTSPGCLHGLCGEPGQCICTDGWDGELCDRDVRACSSAPCANNGTCVSLDDGLYECSCAPGYSGKDCQKKDGPCVINGSPCQHGGTCVDDEGRASHASCLCPPGFSGNFCEIVANSCTPNPCENDGVCTDIGGDFRCRCPAGFIDKTCSRPVTNCASSPCQNGGTCLQHTQVSYECLCKPEFTGLTCVKKRALSPQQVTRLPNGYGLAYRLTPGVHELPVQQPEHRILKVSMKELNKKTPVDHHHHHH","proteinLength":"Fragment","predictedMolecularWeight":"30.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":297,"aminoAcidStart":24,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P80370","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
A few weeks
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Delta-like 1 homolog (DLK-1) also known as Pref-1 (preadipocyte factor 1) is a protein involved in a wide range of cellular processes. The mass of DLK-1 is approximately 65 kDa. This protein is expressed in various tissues including the liver brain and endocrine glands. DLK-1 has an important role in the regulation of cell differentiation and growth acting as a signal molecule that influences tissue development and regeneration.
Biological function summary

DLK-1 serves a function in the inhibition of adipocyte differentiation and the maintenance of preadipocyte phenotype. It does not form part of a complex but interacts with membrane-bound receptors to mediate signaling pathways that impact tissue development. Researchers study its role in embryonic development and its downregulation is a significant factor during the differentiation of mesenchymal cells. Due to these interactions DLK-1 has a pivotal involvement in processes governing tissue regeneration and growth control.

Pathways

Better understanding of DLK-1 involves examining its interaction in the Notch signaling pathway. This important pathway related to cell fate decisions features pathways with other proteins like Notch-1 and Notch-3. The relationship helps modulate the fate and proliferation of cells. DLK-1 also has associations with pathways involving phosphoinositide 3-kinase (PI3K) which influences cell growth and survival through related proteins such as AKT.

DLK-1 has shown associations with cancer and metabolic disorders. Overexpression of DLK-1 is linked to obesity due to its impact on preadipocyte differentiation. Additionally higher levels of DLK-1 can correlate with certain cancer progression where it acts alongside proteins like Notch-1 in promoting tumor cell growth. Understanding DLK-1's role in these contexts highlights the importance of DLK-1 as both a diagnostic marker and potential therapeutic target.
style
left-column

Specifications

Form

Lyophilized

Additional notes

Purity is greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.

style
left-column

General info

Function

May have a role in neuroendocrine differentiation.

Post-translational modifications

N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.

style
left-column

Product protocols

style
left-column

Target data

May have a role in neuroendocrine differentiation.
See full target information DLK1
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed39769389

2025

A Novel Glycoengineered Humanized Antibody Targeting DLK1 Exhibits Potent Anti-Tumor Activity in DLK1-Expressing Liver Cancer Cell Xenograft Models.

Applications

Unspecified application

Species

Unspecified reactive species

Koji Nakamura,Kota Takahashi,Izumi Sakaguchi,Takumi Satoh,Lingyi Zhang,Hiroyuki Yanai,Yukihito Tsukumo

Neoplasia (New York, N.Y.) 22:689-701 PubMed33142235

2020

Niche-derived soluble DLK1 promotes glioma growth.

Applications

Unspecified application

Species

Unspecified reactive species

Elisa S Grassi,Pauline Jeannot,Vasiliki Pantazopoulou,Tracy J Berg,Alexander Pietras
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com