AB255797

Recombinant Human DLL3 protein (His tag)

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(1 Publication)

Recombinant Human DLL3 protein (His tag) is a Human Fragment DLL3 protein, in the 27 to 492 aa range with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, Functional studies and ELISA. The predicted molecular weight of ab255797 recombinant protein is 50.4 kDa.

- Save time and ensure accurate results - us our recombinant DLL3 protein as a control
- Optimal protein bioactivity, stability and reproducibility
- Available in different sizes to fit your experimental needs

View Alternative Names

Delta-like protein 3, Drosophila Delta homolog 3, Delta3, DLL3

4 Images

Functional Studies - Recombinant Human DLL3 protein (His tag) (AB255797)

FuncS

Supplier Data

Functional Studies - Recombinant Human DLL3 protein (His tag) (AB255797)

Bispecific T-cell Engager captured on Protein A Chip can bind Human DLL3,His Tag (ab255797) with an affinity constant of 1.14 nM asdetermined in a SPR assay (Biacore 8K).

ELISA - Recombinant Human DLL3 protein (His tag) (AB255797)

ELISA

Supplier Data

ELISA - Recombinant Human DLL3 protein (His tag) (AB255797)

Immobilized Human DLL3, His Tag (ab255797) at 1 μg/mL (100μL/well) can bind Anti-DLL3 Antibody (specific Binding EGFs of DLL3),Human IgG1 with a linear range of 0.01-2 ng/mL.

ELISA

Supplier Data

ELISA - Recombinant Human DLL3 protein (His tag) (AB255797)

Immobilized Human DLL3, His Tag (ab255797) at 1 μg/mL (100μL/well) can bind Anti-DLL3 Antibody (specific Binding DSL of DLL3), Human IgG1 with a linear range of 0.2-2 ng/mL.

SDS-PAGE - Recombinant Human DLL3 protein (His tag) (AB255797)

SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human DLL3 protein (His tag) (AB255797)

Human DLL3, His Tag (ab255797) on SDS-PAGE under reducing condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Key facts

Purity

>90% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Applications

FuncS, SDS-PAGE, ELISA

applications

Biologically active

Yes

Biological activity

Immobilized Human DLL3, His Tag at 1 μg/mL (100 μL/well) can bind Anti-DLL3 Antibody (specific Binding DSL of DLL3), Human IgG1 with a linear range of 0.2-2 ng/mL.

Accession

Q9NYJ7

Animal free

Carrier free

Species

Human

Reconstitution

Reconstitute in 250 µL of water

Storage buffer

pH: 7.4 Constituents: PBS, 5% Trehalose

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" } } }

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Product details

Ensure the validity of your result using our recombinant human Delta-like protein 3 (DLL3) protein (His tag) ab255797 as a control.

The ab255797 DLL3 protein is sourced from HEK293 cells and can be used as a positive control in SDS-PAGE. Analyze your DLL-3 ELISA data using the ab255797 protein to generate and plot a standard curve.

Check out our protein gel staining guide for SDS-PAGE here

Check out our ELISA protocol for more information here.

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Sequence info

[{"sequence":"AGVFELQIHSFGPGPGPGAPRSPCSARLPCRLFFRVCLKPGLSEEAAESPCALGAALSARGPVYTEQPGAPAPDLPLPDGLLQVPFRDAWPGTFSFIIETWREELGDQIGGPAWSLLARVAGRRRLAAGGPWARDIQRAGAWELRFSYRARCEPPAVGTACTRLCRPRSAPSRCGPGLRPCAPLEDECEAPLVCRAGCSPEHGFCEQPGECRCLEGWTGPLCTVPVSTSSCLSPRGPSSATTGCLVPGPGPCDGNPCANGGSCSETPRSFECTCPRGFYGLRCEVSGVTCADGPCFNGGLCVGGADPDSAYICHCPPGFQGSNCEKRVDRCSLQPCRNGGLCLDLGHALRCRCRAGFAGPRCEHDLDDCAGRACANGGTCVEGGGAHRCSCALGFGGRDCRERADPCAARPCAHGGRCYAHFSGLVCACAPGYMGARCEFPVHPDGASALPAAPPGLRPGDPQRYL","proteinLength":"Fragment","predictedMolecularWeight":"50.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":492,"aminoAcidStart":27,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q9NYJ7","tags":[{"tag":"His","terminus":"C-Terminus"}]}]

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

True

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DLL3 also known as Delta-like protein 3 is a member of the Delta/Serrate/Jagged family. This protein has a molecular mass of approximately 64 kDa. Scientists find DLL3 expression primarily in the Golgi apparatus of cells. In healthy tissues DLL3 is mostly present in the central nervous system whereas in abnormal conditions it appears in certain types of cancer cells. The detection of DLL3 expression commonly performed using IHC (immunohistochemistry) highlights its potential as a biomarker in pathological studies.

Biological function summary

DLL3 functions mainly in the modulation of the Notch signaling pathway. It does not activate this pathway but acts as an inhibitor blocking Notch ligand activation on adjacent cells. DLL3's role involves binding directly to Notch receptors altering cellular differentiation and growth processes. Despite being related to the Delta/Serrate/LAG-2 family DLL3 exhibits unique characteristics in its interactions forming part of a regulatory complex different from its family members.

Pathways

DLL3 engages importantly in the Notch signaling pathway affecting important cellular communications that regulate development and differentiation. Its interaction with proteins of the Notch family specifically the Notch receptors highlights its inhibitory role. Additionally DLL3 intersects with the Wnt signaling pathway though indirect influencing its output by modulating availability of pathway regulators.

DLL3 has significant implications in cancer notably small cell lung cancer (SCLC) and neuroendocrine tumors. Researchers associate increased DLL3 expression with aggressive tumor behavior and poor prognosis. The protein also shows relevance in congenital disorders such as spondylocostal dysostosis where abnormal DLL3 function leads to vertebral segmentation defects. In these contexts DLL3 often relates to proteins involved in the disruption of normal signaling cascades impacting both tumor progression and developmental anomalies.

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Specifications

Form

Lyophilized

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General info

Function

Inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm (By similarity).

Post-translational modifications

Ubiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation.

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information DLL3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed39769389

2025

A Novel Glycoengineered Humanized Antibody Targeting DLK1 Exhibits Potent Anti-Tumor Activity in DLK1-Expressing Liver Cancer Cell Xenograft Models.

Applications

Unspecified application

Species

Unspecified reactive species

Koji Nakamura,Kota Takahashi,Izumi Sakaguchi,Takumi Satoh,Lingyi Zhang,Hiroyuki Yanai,Yukihito Tsukumo

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Recombinant Human DLL3 protein (His tag)

Functional Studies - Recombinant Human DLL3 protein (His tag) (AB255797)

ELISA - Recombinant Human DLL3 protein (His tag) (AB255797)

ELISA - Recombinant Human DLL3 protein (His tag) (AB255797)

SDS-PAGE - Recombinant Human DLL3 protein (His tag) (AB255797)

Key facts

Purity

Endotoxin level

Expression system

Tags

Applications

Biologically active

Biological activity

Accession

Animal free

Carrier free

Species

Reconstitution

Storage buffer

Reactivity data

Product details

Sequence info

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Specifications

Form

General info

Function

Post-translational modifications

Product protocols

Target data

Publications (1)

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