Recombinant human DLL4 protein (Fc Chimera Active) is a Human Fragment protein, in the 1 to 529 aa range, expressed in HEK 293, with >=95% purity, < 0.01 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
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- iScience 25:1043062022Engineered patterns of Notch ligands Jag1 and Dll4 elicit differential spatial control of endothelial sprouting.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaura A Tiemeijer,Tommaso Ristori,Oscar M J A Stassen,Jaakko J Ahlberg,Jonne J J de Bijl,Christopher S Chen,Katie Bentley,Carlijn V C Bouten,Cecilia M SahlgrenPubMed 35602952
- Clinical cancer research : an official journal of the American Association for Cancer Research 26:669-6782019EGFL7 Antagonizes NOTCH Signaling and Represents a Novel Therapeutic Target in Acute Myeloid Leukemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarius Bill,Aparna Pathmanathan,Malith Karunasiri,Changxian Shen,Matthew H Burke,Parvathi Ranganathan,Dimitrios Papaioannou,Nina C Zitzer,Katiri Snyder,Allison LaRocco,Allison E Walker,Zachary J Brannan,Ansel P Nalin,Aharon G Freud,Mikhail M Dikov,Xiaoli Zhang,Clara D Bloomfield,Ramiro Garzon,Adrienne M DorrancePubMed 31672772
- Cellular signalling 54:130-1382018A non-canonical JAGGED1 signal to JAK2 mediates osteoblast commitment in cranial neural crest cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesArchana Kamalakar,Melissa S Oh,Yvonne C Stephenson,Samir A Ballestas-Naissir,Michael E Davis,Nick J Willett,Hicham M Drissi,Steven L GoudyPubMed 30529759
- Scientific reports 8:63922018Spatial patterning of the Notch ligand Dll4 controls endothelial sprouting in vitro.Applications:Unspecified applicationReactive species:Unspecified reactive speciesL A Tiemeijer et. al.PubMed 29686270
- Scientific reports 7:439262017Notch1 Signaling Contributes to Hypoxia-induced High Expression of Integrin β1 in Keratinocyte Migration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDi Tang et. al.PubMed 28266574
- Oncotarget 6:22467-792015DMXL2 drives epithelial to mesenchymal transition in hormonal therapy resistant breast cancer through Notch hyper-activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMonica Faronato,Van T M Nguyen,Darren K Patten,Ylenia Lombardo,Jennifer H Steel,Naina Patel,Laura Woodley,Sami Shousha,Giancarlo Pruneri,R Charles Coombes,Luca MagnaniPubMed 26093085
- The Journal of biological chemistry 289:12016-282014Endothelial Kruppel-like factor 4 regulates angiogenesis and the Notch signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndrew T Hale et. al.PubMed 24599951
Key facts
- Purity
- >=95% SDS-PAGE
- Endotoxin level
- < 0.01 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Fc tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Involved in the Notch signaling pathway as Notch ligand (PubMed:11134954). Activates NOTCH1 and NOTCH4. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting (PubMed:20616313). Essential for retinal progenitor proliferation. Required for suppressing rod fates in late retinal progenitors as well as for proper generation of other retinal cell types (By similarity). During spinal cord neurogenesis, inhibits V2a interneuron fate (PubMed:17728344).
Additional Targets
Alternative names
UNQ1895/PRO4341, DLL4, Delta-like protein 4, Drosophila Delta homolog 4, Delta4
Recommended products
Recombinant human DLL4 protein (Fc Chimera Active) is a Human Fragment protein, in the 1 to 529 aa range, expressed in HEK 293, with >=95% purity, < 0.01 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >=95% SDS-PAGE
- Endotoxin level
- < 0.01 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Fc tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Inhibits adipogenesis of 3T3L-1 cells and mesenchymal stem cells (MSCs). Induces the Notch target gene HES-1 when coated on a plate at 1μg/ml.
- Accession
- Q9NR61-1
- Animal free
- No
- Species
- Human
- Reconstitution
- Reconstitute in 100 µL of water
- Concentration
- Storage buffer
Constituents: PBS, 0.5% Trehalose
Sequence info
Amino acid sequence
- Accession
- Q9NR61
- Protein length
- Fragment
- Predicted molecular weight
- 80 kDa
- Amino acids
- 1 to 529
- Nature
- Recombinant
- Tags
- Fc tag C-Terminus
Specifications
- Form
- Lyophilized
- Additional notes
Purified using affinity chromatography.
General info
- Function
Involved in the Notch signaling pathway as Notch ligand (PubMed:11134954). Activates NOTCH1 and NOTCH4. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting (PubMed:20616313). Essential for retinal progenitor proliferation. Required for suppressing rod fates in late retinal progenitors as well as for proper generation of other retinal cell types (By similarity). During spinal cord neurogenesis, inhibits V2a interneuron fate (PubMed:17728344).
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
ab108557 interacts with Human Notch1 (as confirmed by Flow Cytometry).
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
DLL4 also known as Delta-like ligand 4 is a member of the Delta/Serrate/Lag-2 family. This target is a single-pass type I membrane protein with a molecular weight of approximately 65 kDa. It features prominently in the vascular endothelium where it impacts angiogenic activity. DLL4 acts as a ligand for Notch receptors initiating a signaling cascade important to cell fate decisions. DLL4 expression occurs mainly on the arterial endothelium and the tumor vasculature highlighting its significance in angiogenesis.
- Biological function summary
DLL4 engages in precise regulation of angiogenesis and vascular development. It participates in the Notch signaling pathway playing an integral role in controlling endothelial cell proliferation differentiation and migration. DLL4 does not operate in isolation; instead it functions as part of the Notch receptor-ligand complex. This relationship effectively regulates the sprouting of new blood vessels ensuring proper vascular patterning and functional blood supply.
- Pathways
DLL4 links directly to the Notch signaling and VEGF (vascular endothelial growth factor) pathways both of which play critical roles in vascular development and homeostasis. Through the Notch pathway DLL4 interacts with Notch1 and Notch4 receptors facilitating communication that inhibits endothelial cell proliferation distinguishing it from other pro-angiogenic factors. Its relationship with the VEGF pathway allows DLL4 to modulate angiogenic processes balancing vessel sprouting by opposing excessive VEGF-induced activities.
- Associated diseases and disorders
Aberrations of DLL4 influence cancer and cardiovascular diseases. Overexpression and dysregulation of DLL4 have been observed in various cancers where it contributes to tumor angiogenesis promoting the growth and spread of cancer cells. In cardiovascular disorders dysfunctional DLL4 signaling impacts vascular development which can lead to fatal arteriovenous malformations. In these conditions DLL4 interacts with proteins like Notch1 and VEGF underlining its critical involvement in pathological angiogenesis and vessel formation.
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Functional Studies - Recombinant human DLL4 protein (Fc Chimera Active) (ab108557)
Interaction of Human Notch1 with Human DLL4.
HEK293 cells transfected with a Human Notch1 or a Human GITR ligand expressing vector were incubated with 25 μg/ml of Human GITR-Fc or ab108557. Cells were stained with anti-Human IgG (Fc specific) FITC conjugate for DLL4-Fc binding.
Functional Studies - Recombinant human DLL4 protein (Fc Chimera Active) (ab108557)
Induction of Hes-1 with the treatment of recombinant Human DLL4-Fc (ab108557).
A Mouse preadpipocyte cell line, 3T3L-1, was stimulated with 5 μg/ml of Human DLL4-Fc as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-Mouse Hes1 or GAPDH.
M: Marker.
Lane 1: hDLL4-Fc, 0 min.
Lane 2: hDLL4-Fc, 10 min.
Lane 3: hDLL4-Fc, 30 min.
Lane 4: hDLL4-Fc, 1 hr.
Lane 5: hDLL4-Fc, 2 hr.
Lane 6: hDLL4-Fc, 4 hr.
Lane 7: hDLL4-Fc, 8 hr.
Lane 8: hDLL4-Fc, 24 hr.
Functional Studies - Recombinant human DLL4 protein (Fc Chimera Active) (ab108557)
Adipogenesis inhibition of 3T3L-1 cells.
3T3L-1 cells (mouse pre-adipocyte cells) were maintained in DMEM, supplemented with 10% fetal bovine serum
and penicillin-streptomycin. For differentiation of 3T3L-1 cells, 3T3L-1 cells were cultured in adipogenic medium
which was growth medium supplemented with 1 μM Dexamethasone, 0.5 mM IBMX, 10 μg/ml lnsulin (day 0).
Medium was changed every 2 days. Staining with Oil Red O was typically performed on day 7. Cells were
washed twice with PBS, fixed with 3.7% formalin, and stained with 0.5% filtered Oil Red O in propylene glycol.
For negative controls, mouse TNF-α (20 ng/ml) was added. Recombinant Human DLL4-Fc (ab108557) (5 μg/ml) dissolved in
DPBS was added to the differentiation medium. These plates were then used to differentiate 3T3L-1 cells.
Functional Studies - Recombinant human DLL4 protein (Fc Chimera Active) (ab108557)
Adipogenesis inhibition of MSCs.
MSCs (Mesenchymal stem cells) were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilinstreptomycin and glutamine. For differentiation of MSCs, MSCs were cultured in adipogenic medium which was
growth medium supplemented with 1 μM Dexamethasone, 0.5mM IBMX, 10 μg/m lnsulin, 100 μM Indomethacin (day 1). Medium was changed every 3 days. Staining with Oil Red O was typically performed on day 30. For negative controls, TNF-α (20 ng/ml) was added. To immobilize Notch ligands on the plastic surface of the culture plates, plates were incubated with a solution of ab108557 (5 μg/ml) or mCD137-Fc (5 μg/ml) in PBS for 2 hours at 37°C. Plates were then used to differentiate MSCs.
Functional Studies - Recombinant human DLL4 protein (Fc Chimera Active) (ab108557)
Adipogenesis inhibition of 3T3L-1 cells.
Functional Studies - Recombinant human DLL4 protein (Fc Chimera Active) (ab108557)
50 μg of cell lysates derived from hDLL4-Fc (ab108557) or non-treated 3T3L-1 cells, which had been either differentiated or undifferentiated, and were subjected to Western blot by using a Mouse adiponectin antibody.
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