Recombinant Human DNA polymerase eta protein

Specifications

Form

Liquid

General info

Function

DNA polymerase specifically involved in the DNA repair by translesion synthesis (TLS) (PubMed : 10385124, PubMed : 11743006, PubMed : 16357261, PubMed : 24449906, PubMed : 24553286, PubMed : 38212351). Due to low processivity on both damaged and normal DNA, cooperates with the heterotetrameric (REV3L, REV7, POLD2 and POLD3) POLZ complex for complete bypass of DNA lesions. Inserts one or 2 nucleotide(s) opposite the lesion, the primer is further extended by the tetrameric POLZ complex. In the case of 1,2-intrastrand d(GpG)-cisplatin cross-link, inserts dCTP opposite the 3' guanine (PubMed : 24449906). Particularly important for the repair of UV-induced pyrimidine dimers (PubMed : 10385124, PubMed : 11743006). Although inserts the correct base, may cause base transitions and transversions depending upon the context. May play a role in hypermutation at immunoglobulin genes (PubMed : 11376341, PubMed : 14734526). Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have any lyase activity, preventing the release of the 5'-deoxyribose phosphate (5'-dRP) residue. This covalent trapping of the enzyme by the 5'-dRP residue inhibits its DNA synthetic activity during base excision repair, thereby avoiding high incidence of mutagenesis (PubMed : 14630940). Targets POLI to replication foci (PubMed : 12606586).

Sequence similarities

Belongs to the DNA polymerase type-Y family.

Post-translational modifications

Monoubiquitinated by RCHY1/PIRH2 (PubMed:20159558, PubMed:21791603). Ubiquitination depends on integrity of the UBZ3-type zinc finger domain and is enhanced by TRAIP (PubMed:16357261, PubMed:24553286). Ubiquitination inhibits the ability of PolH to interact with PCNA and to bypass UV-induced lesions (PubMed:20159558, PubMed:21791603, PubMed:24553286).

Subcellular localisation

Nucleus