Recombinant human DNA Polymerase Kappa/POLK protein
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Recombinant human DNA Polymerase Kappa/POLK protein is a Human Fragment protein, in the 1 to 560 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, ELISA, WB, FuncS.
View Alternative Names
DINB1, POLK, DNA polymerase kappa, DINB protein, DINP
- FuncS
Supplier Data
Functional Studies - Recombinant human DNA Polymerase Kappa/POLK protein (AB176956)
DNA polymerizing activity of ab176956
50 mM Tris-HCl pH 8.0, 2 mM MgCl2, 1 mM DTT, 0.1 mg / ml BSA, 0.1 mM dNTP, 100 nM primer/template(13-mer/30-mer), DNA polymerase kappa/POLK at 13~50 ng/25μl (lanes 1,2, and 3 respectiviely) per assay, incubated at 37℃ for 10 min.
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant human DNA Polymerase Kappa/POLK protein (AB176956)
SDS-PAGE analysis of ab196956.
Complementary Products
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
DNA polymerase kappa plays a significant role in error-prone repair mechanisms contributing to DNA damage tolerance. It is not known to associate directly with a larger complex but works in concert with other translesion synthesis polymerases such as DNA polymerase eta. Through its action POLK introduces nucleotides opposite DNA lesions maintaining replication fork progression and cell viability under genotoxic stress conditions.
Pathways
DNA polymerase kappa is integral to the translesion synthesis pathway and participates in the broader DNA damage response system. It relates functionally to other DNA polymerases including polymerase eta and zeta which together modulate cellular responses to DNA damage and repair. The interplay between these enzymes facilitates flexible and adaptive responses to various genotoxic insults highlighting their roles in maintaining genomic stability.
Specifications
Form
Liquid
General info
Function
DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. Lacks 3'-5' proofreading exonuclease activity. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity.
Sequence similarities
Belongs to the DNA polymerase type-Y family.
Subcellular localisation
Nucleus
Target data
Product promise
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