Recombinant human DNA Polymerase Kappa/POLK protein is a Human Fragment protein, in the 1 to 560 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, ELISA, WB, FuncS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, ELISA, WB, FuncS
- Biologically active
- Yes
Amino acid sequence
M D S T K E K C D S Y K D D L L L R M G L N D N K A G M E G L D K E K I N K I I M E A T K G S R F Y G N E L K K E K Q V N Q R I E N M M Q Q K A Q I T S Q Q L R K A Q L Q V D R F A M E L E Q S R N L S N T I V H I D M D A F Y A A V E M R D N P E L K D K P I A V G S M S M L S T S N Y H A R R F G V R A A M P G F I A K R L C P Q L I I V P P N F D K Y R A V S K E V K E I L A D Y D P N F M A M S L D E A Y L N I T K H L E E R Q N W P E D K R R Y F I K M G S S V E N D N P G K E V N K L S E H E R S I S P L L F E E S P S D V Q P P G D P F Q V N F E E Q N N P Q I L Q N S V V F G T S A Q E V V K E I R F R I E Q K T T L T A S A G I A P N T M L A K V C S D K N K P N G Q Y Q I L P N R Q A V M D F I K D L P I R K V S G I G K V T E K M L K A L G I I T C T E L Y Q Q R A L L S L L F S E T S W H Y F L H I S L G L G S T H L T R D G E R K S M S V E R T F S E I N K A E E Q Y S L C Q E L C S E L A Q D L Q K E R L K G R T V T I K L K N V N F E V K T R A S T V S S V V S T A E E I F A I A K E L L K T E I D A D F P H P L R L R L M G V R I S S F P N E E D R K H Q Q R S I I G F L Q A G N Q A L S A T E C T L E K T D K D K F V K P L E
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. Lacks 3'-5' proofreading exonuclease activity. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity.
Alternative names
DINB1, POLK, DNA polymerase kappa, DINB protein, DINP
Recommended products
Recombinant human DNA Polymerase Kappa/POLK protein is a Human Fragment protein, in the 1 to 560 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, ELISA, WB, FuncS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, ELISA, WB, FuncS
- Biologically active
- Yes
- Biological activity
- Active
- Accession
- Q9UBT6-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 6 - 8.5
Constituents: 50% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.14% Sodium phosphate
Sequence info
Amino acid sequence
- M D S T K E K C D S Y K D D L L L R M G L N D N K A G M E G L D K E K I N K I I M E A T K G S R F Y G N E L K K E K Q V N Q R I E N M M Q Q K A Q I T S Q Q L R K A Q L Q V D R F A M E L E Q S R N L S N T I V H I D M D A F Y A A V E M R D N P E L K D K P I A V G S M S M L S T S N Y H A R R F G V R A A M P G F I A K R L C P Q L I I V P P N F D K Y R A V S K E V K E I L A D Y D P N F M A M S L D E A Y L N I T K H L E E R Q N W P E D K R R Y F I K M G S S V E N D N P G K E V N K L S E H E R S I S P L L F E E S P S D V Q P P G D P F Q V N F E E Q N N P Q I L Q N S V V F G T S A Q E V V K E I R F R I E Q K T T L T A S A G I A P N T M L A K V C S D K N K P N G Q Y Q I L P N R Q A V M D F I K D L P I R K V S G I G K V T E K M L K A L G I I T C T E L Y Q Q R A L L S L L F S E T S W H Y F L H I S L G L G S T H L T R D G E R K S M S V E R T F S E I N K A E E Q Y S L C Q E L C S E L A Q D L Q K E R L K G R T V T I K L K N V N F E V K T R A S T V S S V V S T A E E I F A I A K E L L K T E I D A D F P H P L R L R L M G V R I S S F P N E E D R K H Q Q R S I I G F L Q A G N Q A L S A T E C T L E K T D K D K F V K P L E
- Accession
- Q9UBT6
- Protein length
- Fragment
- Predicted molecular weight
- 64 kDa
- Amino acids
- 1 to 560
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Liquid
General info
- Function
DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. Lacks 3'-5' proofreading exonuclease activity. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity.
- Sequence similarities
Belongs to the DNA polymerase type-Y family.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
DNA polymerase kappa also known as POLK or kappa polymerase is a specialized enzyme involved in DNA replication and repair. This protein weighs approximately 93 kDa. POLK is expressed across various human tissues with notable activity in testis and placenta. Mechanically DNA polymerase kappa participates in translesion synthesis a process that permits DNA replication to proceed past damaged or abnormal bases allowing cells to overcome blockages that conventional DNA polymerases cannot bypass.
- Biological function summary
DNA polymerase kappa plays a significant role in error-prone repair mechanisms contributing to DNA damage tolerance. It is not known to associate directly with a larger complex but works in concert with other translesion synthesis polymerases such as DNA polymerase eta. Through its action POLK introduces nucleotides opposite DNA lesions maintaining replication fork progression and cell viability under genotoxic stress conditions.
- Pathways
DNA polymerase kappa is integral to the translesion synthesis pathway and participates in the broader DNA damage response system. It relates functionally to other DNA polymerases including polymerase eta and zeta which together modulate cellular responses to DNA damage and repair. The interplay between these enzymes facilitates flexible and adaptive responses to various genotoxic insults highlighting their roles in maintaining genomic stability.
- Associated diseases and disorders
DNA polymerase kappa's association with carcinogenesis is of particular interest. Its error-prone activity can result in mutagenesis linking it to cancer development. POLK's expression or malfunction influences conditions like xeroderma pigmentosum where defective DNA repair increases cancer risk. Connections with the p53 pathway illustrate that defects in related DNA repair proteins can exacerbate the development of neoplastic diseases highlighting considerable implications for targeted cancer therapies.
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2 product images
Functional Studies - Recombinant human DNA Polymerase Kappa/POLK protein (ab176956)
DNA polymerizing activity of ab176956
50 mM Tris-HCl pH 8.0, 2 mM MgCl2, 1 mM DTT, 0.1 mg / ml BSA, 0.1 mM dNTP, 100 nM primer/template(13-mer/30-mer), DNA polymerase kappa/POLK at 13~50 ng/25μl (lanes 1,2, and 3 respectiviely) per assay, incubated at 37℃ for 10 min.
SDS-PAGE - Recombinant human DNA Polymerase Kappa/POLK protein (ab176956)
SDS-PAGE analysis of ab196956.
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