Recombinant Human DNA Polymerase lambda protein
Be the first to review this product! Submit a review
|
(0 Publication)
Recombinant Human DNA Polymerase lambda protein is a Human Full Length protein, in the 1 to 300 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.
View Alternative Names
DNA polymerase lambda, Pol Lambda, DNA polymerase beta-2, DNA polymerase kappa, Pol beta2, POLL
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant Human DNA Polymerase lambda protein (AB113399)
15% SDS-PAGE of DNA Polymerase lambda/Polk protein : (1) MW ladder, (2) protein loaded 3ug
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
DNA polymerase lambda acts as a gap-filling enzyme during DNA repair processes especially in base excision repair and non-homologous end joining. This polymerase does not typically function within a larger complex but requires accessory factors to perform its repair functions. It maintains genomic stability by providing an accurate extension of DNA strands during repair ensuring the correct rejoining of DNA. Furthermore it also exhibits a lyase activity that can remove 5'-deoxyribose phosphate residues.
Pathways
DNA polymerase lambda is actively involved in the base excision repair and non-homologous end joining pathways. These pathways are important for fixing DNA single-strand breaks and double-strand breaks respectively. Pol λ works alongside proteins like XRCC1 in the short-patch base excision repair pathway and interacts with KU70/80 in non-homologous end joining reinforcing its multifaceted role in maintaining DNA integrity under varying genotoxic stress conditions.
Specifications
Form
Liquid
Additional notes
Conventional chromatography techniques (anion exchange followed by gel filtration with 20mM Tris pH 7.5, 2mM EDTA).
General info
Function
DNA polymerase that functions in several pathways of DNA repair (PubMed : 11457865, PubMed : 19806195, PubMed : 20693240, PubMed : 30250067). Involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA (PubMed : 11457865, PubMed : 19806195). Also contributes to DNA double-strand break repair by non-homologous end joining and homologous recombination (PubMed : 19806195, PubMed : 20693240, PubMed : 30250067). Has both template-dependent and template-independent (terminal transferase) DNA polymerase activities (PubMed : 10887191, PubMed : 10982892, PubMed : 12809503, PubMed : 14627824, PubMed : 15537631, PubMed : 19806195). Has also a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity (PubMed : 11457865, PubMed : 19806195).
Sequence similarities
Belongs to the DNA polymerase type-X family.
Subcellular localisation
Nucleus
Target data
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com