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AB113174

Recombinant Human DNAJA1 protein

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Recombinant Human DNAJA1 protein is a Human Full Length protein, in the 1 to 394 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, WB.

View Alternative Names

DNAJ2, HDJ2, HSJ2, HSPF4, DNAJA1, DnaJ homolog subfamily A member 1, DnaJ protein homolog 2, HSDJ, Heat shock 40 kDa protein 4, Heat shock protein J2, Human DnaJ protein 2, HSJ-2, hDj-2

2 Images
Western blot - Recombinant Human DNAJA1 protein (AB113174)
  • WB

Unknown

Western blot - Recombinant Human DNAJA1 protein (AB113174)

Western Blot analysis
Lane 1 : MW marker
Lane 2 : DNAJA1 (ab113174) probed with anti-DNAJA1 monoclonal antibody.

All lanes:

Western blot - Recombinant Human DNAJA1 protein (ab113174)

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SDS-PAGE - Recombinant Human DNAJA1 protein (AB113174)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human DNAJA1 protein (AB113174)

SDS-PAGE analysis
Lane 1 : MW marker
Lane 2 : 0.5 μg DNAJA1 protein (ab113174)
Lane 3 : 1 μg DNAJA1 protein (ab113174)
Lane 4 : 2 μg DNAJA1 protein (ab113174)
Lane 5 : 5 μg DNAJA1 protein (ab113174).

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE, WB

applications

Biologically active

No

Accession

P31689

Animal free

No

Carrier free

No

Species

Human

Storage buffer

Constituents: 99% Tris buffered saline, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>(control, 100 ng, colorimetric).</p>" } } }
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Product details

This product was previously labelled as HDJ2
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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"45 kDa","actualMolecularWeight":null,"aminoAcidEnd":394,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P31689","tags":[]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

Additional notes

ab113174 was purified by multi-step chromatography.

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General info

Function

Co-chaperone for HSPA8/Hsc70 (PubMed : 10816573). Stimulates ATP hydrolysis, but not the folding of unfolded proteins mediated by HSPA1A (in vitro) (PubMed : 24318877). Plays a role in protein transport into mitochondria via its role as co-chaperone. Functions as a co-chaperone for HSPA1B and negatively regulates the translocation of BAX from the cytosol to mitochondria in response to cellular stress, thereby protecting cells against apoptosis (PubMed : 14752510). Promotes apoptosis in response to cellular stress mediated by exposure to anisomycin or UV (PubMed : 24512202).

Subcellular localisation

Nucleus

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Product protocols

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Target data

Co-chaperone for HSPA8/Hsc70 (PubMed : 10816573). Stimulates ATP hydrolysis, but not the folding of unfolded proteins mediated by HSPA1A (in vitro) (PubMed : 24318877). Plays a role in protein transport into mitochondria via its role as co-chaperone. Functions as a co-chaperone for HSPA1B and negatively regulates the translocation of BAX from the cytosol to mitochondria in response to cellular stress, thereby protecting cells against apoptosis (PubMed : 14752510). Promotes apoptosis in response to cellular stress mediated by exposure to anisomycin or UV (PubMed : 24512202).
See full target information DNAJA1
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Complementary Products

Primary Antibodies

AB126774

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Western blot - Anti-DNAJA1 antibody [EPR7248] (AB126774)

  • 0
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Primary Antibodies

AB168829

Anti-NKRF antibody

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Cell Lines & Lysates

AB257925

Human DNAJA1 knockout HEK-293T cell lysate

Western blot - Human DNAJA1 knockout HEK-293T cell lysate (AB257925)

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Cell Lines & Lysates

AB266437

Human DNAJA1 knockout HEK-293T cell line

Advanced Validation

Western blot - Human DNAJA1 knockout HEK-293T cell line (AB266437)

  • 0
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Product promise

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