Recombinant Human DNase gamma protein (Tagged) is a Human Full Length protein, in the 21 to 305 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M R I C S F N V R S F G E S K Q E D K N A M D V I V K V I K R C D I I L V M E I K D S N N R I C P I L M E K L N R N S R R G I T Y N Y V I S S R L G R N T Y K E Q Y A F L Y K E K L V S V K R S Y H Y H D Y Q D G D A D V F S R E P F V V W F Q S P H T A V K D F V I I P L H T T P E T S V K E I D E L V E V Y T D V K H R W K A E N F I F M G D F N A G C S Y V P K K A W K N I R L R T D P R F V W L I G D Q E D T T V K K S T N C A Y D R I V L R G Q E I V S S V V P K S N S V F D F Q K A Y K L T E E E A L D V S D H F P V E F K L Q S S R A F T N S K K S V T L R K K T K S K R S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Has DNA hydrolytic activity. Is capable of both single- and double-stranded DNA cleavage, producing DNA fragments with 3'-OH ends (By similarity). Can cleave chromatin to nucleosomal units and cleaves nucleosomal and liposome-coated DNA (PubMed:10807908, PubMed:14646506, PubMed:27293190, PubMed:9070308, PubMed:9714828). Acts in internucleosomal DNA fragmentation (INDF) during apoptosis and necrosis (PubMed:23229555, PubMed:24312463). The role in apoptosis includes myogenic and neuronal differentiation, and BCR-mediated clonal deletion of self-reactive B cells (By similarity). Is active on chromatin in apoptotic cell-derived membrane-coated microparticles and thus suppresses anti-DNA autoimmunity (PubMed:27293190). Together with DNASE1, plays a key role in degrading neutrophil extracellular traps (NETs) (By similarity). NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation (By similarity). Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation (By similarity).
Alternative names
DHP2, DNAS1L3, DNASE1L3, Deoxyribonuclease gamma, DNase gamma, DNase I homolog protein DHP2, Deoxyribonuclease I-like 3, Liver and spleen DNase, DNase I-like 3, LS-DNase, LSD
Recommended products
Recombinant Human DNase gamma protein (Tagged) is a Human Full Length protein, in the 21 to 305 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q13609-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- M R I C S F N V R S F G E S K Q E D K N A M D V I V K V I K R C D I I L V M E I K D S N N R I C P I L M E K L N R N S R R G I T Y N Y V I S S R L G R N T Y K E Q Y A F L Y K E K L V S V K R S Y H Y H D Y Q D G D A D V F S R E P F V V W F Q S P H T A V K D F V I I P L H T T P E T S V K E I D E L V E V Y T D V K H R W K A E N F I F M G D F N A G C S Y V P K K A W K N I R L R T D P R F V W L I G D Q E D T T V K K S T N C A Y D R I V L R G Q E I V S S V V P K S N S V F D F Q K A Y K L T E E E A L D V S D H F P V E F K L Q S S R A F T N S K K S V T L R K K T K S K R S
- Accession
- Q13609
- Protein length
- Full Length
- Predicted molecular weight
- 60.4 kDa
- Amino acids
- 21 to 305
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Has DNA hydrolytic activity. Is capable of both single- and double-stranded DNA cleavage, producing DNA fragments with 3'-OH ends (By similarity). Can cleave chromatin to nucleosomal units and cleaves nucleosomal and liposome-coated DNA (PubMed:10807908, PubMed:14646506, PubMed:27293190, PubMed:9070308, PubMed:9714828). Acts in internucleosomal DNA fragmentation (INDF) during apoptosis and necrosis (PubMed:23229555, PubMed:24312463). The role in apoptosis includes myogenic and neuronal differentiation, and BCR-mediated clonal deletion of self-reactive B cells (By similarity). Is active on chromatin in apoptotic cell-derived membrane-coated microparticles and thus suppresses anti-DNA autoimmunity (PubMed:27293190). Together with DNASE1, plays a key role in degrading neutrophil extracellular traps (NETs) (By similarity). NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation (By similarity). Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation (By similarity).
- Sequence similarities
Belongs to the DNase I family.
- Post-translational modifications
Poly-ADP-ribosylated by PARP1. ADP-ribosylation negatively regulates enzymatic activity during apoptosis.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
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1 product image
SDS-PAGE - Recombinant Human DNase gamma protein (Tagged) (ab238220)
ab238220 analyzed by (Tris-Glycine gel) discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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