Recombinant Human DNase I protein (His tag) is a Human Full Length protein, in the 1 to 282 aa range, expressed in HEK 293, with >85% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >85% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M R G M K L L G A L L A L A A L L Q G A V S L K I A A F N I Q T F G E T K M S N A T L V S Y I V Q I L S R Y D I A L V Q E V R D S H L T A V G K L L D N L N Q D A P D T Y H Y V V S E P L G R N S Y K E R Y L F V Y R P D Q V S A V D S Y Y Y D D G C E P C G N D T F N R E P A I V R F F S R F T E V R E F A I V P L H A A P G D A V A E I D A L Y D V Y L D V Q E K W G L E D V M L M G D F N A G C S Y V R P S Q W S S I R L W T S P T F Q W L I P D S A D T T A T P T H C A Y D R I V V A G M L L R G A V V P D S A L P F N F Q A A Y G L S D Q L A Q A I S D H Y P V E V M L K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Serum endocuclease secreted into body fluids by a wide variety of exocrine and endocrine organs (PubMed:11241278, PubMed:2251263, PubMed:2277032). Expressed by non-hematopoietic tissues and preferentially cleaves protein-free DNA (By similarity). Among other functions, seems to be involved in cell death by apoptosis (PubMed:11241278). Binds specifically to G-actin and blocks actin polymerization (By similarity). Together with DNASE1L3, plays a key role in degrading neutrophil extracellular traps (NETs) (By similarity). NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation (By similarity). Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation (By similarity).
Alternative names
DNL1, DRNI, DNASE1, Deoxyribonuclease-1, Deoxyribonuclease I, DNase I
Recommended products
Recombinant Human DNase I protein (His tag) is a Human Full Length protein, in the 1 to 282 aa range, expressed in HEK 293, with >85% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >85% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P24855-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 100% PBS
Sequence info
Amino acid sequence
- M R G M K L L G A L L A L A A L L Q G A V S L K I A A F N I Q T F G E T K M S N A T L V S Y I V Q I L S R Y D I A L V Q E V R D S H L T A V G K L L D N L N Q D A P D T Y H Y V V S E P L G R N S Y K E R Y L F V Y R P D Q V S A V D S Y Y Y D D G C E P C G N D T F N R E P A I V R F F S R F T E V R E F A I V P L H A A P G D A V A E I D A L Y D V Y L D V Q E K W G L E D V M L M G D F N A G C S Y V R P S Q W S S I R L W T S P T F Q W L I P D S A D T T A T P T H C A Y D R I V V A G M L L R G A V V P D S A L P F N F Q A A Y G L S D Q L A Q A I S D H Y P V E V M L K
- Accession
- P24855
- Protein length
- Full Length
- Predicted molecular weight
- 30.7 kDa
- Amino acids
- 1 to 282
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Serum endocuclease secreted into body fluids by a wide variety of exocrine and endocrine organs (PubMed:11241278, PubMed:2251263, PubMed:2277032). Expressed by non-hematopoietic tissues and preferentially cleaves protein-free DNA (By similarity). Among other functions, seems to be involved in cell death by apoptosis (PubMed:11241278). Binds specifically to G-actin and blocks actin polymerization (By similarity). Together with DNASE1L3, plays a key role in degrading neutrophil extracellular traps (NETs) (By similarity). NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation (By similarity). Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation (By similarity).
- Sequence similarities
Belongs to the DNase I family.
- Subcellular localisation
- Nucleus envelope
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
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1 product image
SDS-PAGE - Recombinant Human DNase I protein (His tag) (ab276591)
SDS-PAGE analysis of ab276591
Downloads
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