Recombinant human Dnmt1 protein

Key facts

Purity

>95% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

His-DDDDK tag N-Terminus

Applications

SDS-PAGE, FuncS

Biologically active

Yes

Biological activity

Specific Activity: ≥0.16 pmol/min/μg.

Assay Conditions: Assay was performed using a DNMT1 Assay kit, using 40 μM S-adenosylmethionine for 1 hour at 37°C.

Accession

P26358-1

Animal free

No

Species

Human

Concentration

Storage buffer

pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.02% Potassium chloride

Sequence info

Amino acid sequence

M H H H H H H D Y K D D D D K E Y I L M D P S P E Y A P I F G L M Q E K I Y I S K I V V E F L Q S N S D S T Y E D L I N K I E T T V P P S G L N L N R F T E D S L L R H A Q F V V E Q V E S Y D E A G D S D E Q P I F L T P C M R D L I K L A G V T L G Q R R A Q A R R Q T I R H S T R E K D R G P T K A T T T K L V Y Q I F D T F F A E Q I E K D D R E D K E N A F K R R R C G V C E V C Q Q P E C G K C K A C K D M V K F G G S G R S K Q A C Q E R R C P N M A M K E A D D D E E V D D N I P E M P S P K K M H Q G K K K K Q N K N R I S W V G E A V K T D G K K S Y Y K K V C I D A E T L E V G D C V S V I P D D S S K P L Y L A R V T A L W E D S S N G Q M F H A H W F C A G T D T V L G A T S D P L E L F L V D E C E D M Q L S Y I H S K V K V I Y K A P S E N W A M E G G M D P E S L L E G D D G K T Y F Y Q L W Y D Q D Y A R F E S P P K T Q P T E D N K F K F C V S C A R L A E M R Q K E I P R V L E Q L E D L D S R V L Y Y S A T K N G I L Y R V G D G V Y L P P E A F T F N I K L S S P V K R P R K E P V D E D L Y P E H Y R K Y S D Y I K G S N L D A P E P Y R I G R I K E I F C P K K S N G R P N E T D I K I R V N K F Y R P E N T H K S T P A S Y H A D I N L L Y W S D E E A V V D F K A V Q G R C T V E Y G E D L P E C V Q V Y S M G G P N R F Y F L E A Y N A K S K S F E D P P N H A R S P G N K G K G K G K G K G K P K S Q A C E P S E P E I E I K L P K L R T L D V F S G C G G L S E G F H Q A G I S D T L W A I E M W D P A A Q A F R L N N P G S T V F T E D C N I L L K L V M A G E T T N S R G Q R L P Q K G D V E M L C G G P P C Q G F S G M N R F N S R T Y S K F K N S L V V S F L S Y C D Y Y R P R F F L L E N V R N F V S F K R S M V L K L T L R C L V R M G Y Q C T F G V L Q A G Q Y G V A Q T R R R A I I L A A A P G E K L P L F P E P L H V F A P R A C Q L S V V V D D K K F V S N I T R L S S G P F R T I T V R D T M S D L P E V R N G A S A L E I S Y N G E P Q S W F Q R Q L R G A Q Y Q P I L R D H I C K D M S A L V A A R M R H I P L A P G S D W R D L P N I E V R L S D G T M A R K L R Y T H H D R K N G R S S S G A L R G V C S C V E A G K A C D P A A R Q F N T L I P W C L P H T G N R H N H W A G L Y G R L E W D G F F S T T V T N P E P M G K Q G R V L H P E Q H R V V S V R E C A R S Q G F P D T Y R L F G N I L D K H R Q V G N A V P P P L A K A I G L E I K L C M L A K A R E S A S A K I K E E E A A K D

Accession

P26358

Protein length

Fragment

Predicted molecular weight

130 kDa

Amino acids

501 to 1632

Nature

Recombinant

Tags

His-DDDDK tag N-Terminus

Notes

Product promise