Recombinant Human Dnmt2 protein is a Human Full Length protein, in the 1 to 391 aa range, expressed in Baculovirus infected Sf9, with >95% purity and suitable for SDS-PAGE, WB.
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Key facts
- Purity
- >95% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, WB
- Biologically active
- No
Amino acid sequence
M E P L R V L E L Y S G V G G M H H A L R E S C I P A Q V V A A I D V N T V A N E V Y K Y N F P H T Q L L A K T I E G I T L E E F D R L S F D M I L M S P P C Q P F T R I G R Q G D M T D S R T N S F L H I L D I L P R L Q K L P K Y I L L E N V K G F E V S S T R D L L I Q T I E N C G F Q Y Q E F L L S P T S L G I P N S R L R Y F L I A K L Q S E P L P F Q A P G Q V L M E F P K I E S V H P Q K Y A M D V E N K I Q E K N V E P N I S F D G S I Q C S G K D A I L F K L E T A E E I H R K N Q Q D S D L S V K M L K D F L E D D T D V N Q Y L L P P K S L L R Y A L L L D I V Q P T C R R S V C F T K G Y G S Y I E G T G S V L Q T A E D V Q V E N I Y K S L T N L S Q E E Q I T K L L I L K L R Y F T P K E I A N L L G F P P E F G F P E K I T V K Q R Y R L L G N S L N V H V V A K L I K I L Y E
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Specifically methylates cytosine 38 in the anticodon loop of tRNA(Asp) (PubMed:16424344). Has higher activity on tRNA(Asp) modified with queuosine at position 34 (PubMed:30093495).
Alternative names
DNMT2, TRDMT1, tRNA (cytosine(38)-C(5))-methyltransferase, DNA (cytosine-5)-methyltransferase-like protein 2, DNA methyltransferase homolog HsaIIP, PuMet, Dnmt2, DNA MTase homolog HsaIIP, M.HsaIIP
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Recombinant Human Dnmt2 protein is a Human Full Length protein, in the 1 to 391 aa range, expressed in Baculovirus infected Sf9, with >95% purity and suitable for SDS-PAGE, WB.
Key facts
- Purity
- >95% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, WB
- Biologically active
- No
- Accession
- O14717-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF
Sequence info
Amino acid sequence
- M E P L R V L E L Y S G V G G M H H A L R E S C I P A Q V V A A I D V N T V A N E V Y K Y N F P H T Q L L A K T I E G I T L E E F D R L S F D M I L M S P P C Q P F T R I G R Q G D M T D S R T N S F L H I L D I L P R L Q K L P K Y I L L E N V K G F E V S S T R D L L I Q T I E N C G F Q Y Q E F L L S P T S L G I P N S R L R Y F L I A K L Q S E P L P F Q A P G Q V L M E F P K I E S V H P Q K Y A M D V E N K I Q E K N V E P N I S F D G S I Q C S G K D A I L F K L E T A E E I H R K N Q Q D S D L S V K M L K D F L E D D T D V N Q Y L L P P K S L L R Y A L L L D I V Q P T C R R S V C F T K G Y G S Y I E G T G S V L Q T A E D V Q V E N I Y K S L T N L S Q E E Q I T K L L I L K L R Y F T P K E I A N L L G F P P E F G F P E K I T V K Q R Y R L L G N S L N V H V V A K L I K I L Y E
- Accession
- O14717
- Protein length
- Full Length
- Predicted molecular weight
- 67 kDa
- Amino acids
- 1 to 391
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Specifically methylates cytosine 38 in the anticodon loop of tRNA(Asp) (PubMed:16424344). Has higher activity on tRNA(Asp) modified with queuosine at position 34 (PubMed:30093495).
- Sequence similarities
Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
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1 product image
SDS-PAGE - Recombinant Human Dnmt2 protein (ab136359)
SDS-PAGE using ab136359
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Product protocols
- Visit the general protocols
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