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AB198133

Recombinant human DNMT3B + DNMT3L protein (GST tag N-Terminus, His tag N-Terminus)

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Recombinant human DNMT3B + DNMT3L protein (GST tag N-Terminus, His tag N-Terminus) is a Human Fragment protein, in the 564 to 853 aa range, expressed in Baculovirus infected Sf9 cells, with >85%, suitable for SDS-PAGE, FuncS.

View Alternative Names

DNA (cytosine-5)-methyltransferase 3B, Dnmt3b, DNA methyltransferase HsaIIIB, DNA MTase HsaIIIB, M.HsaIIIB, DNMT3B, DNA (cytosine-5)-methyltransferase 3-like, DNMT3L

2 Images
Functional Studies - Recombinant human DNMT3B + DNMT3L protein (GST tag N-Terminus, His tag N-Terminus) (AB198133)
  • FuncS

Supplier Data

Functional Studies - Recombinant human DNMT3B + DNMT3L protein (GST tag N-Terminus, His tag N-Terminus) (AB198133)

Functional assay of ab198133.

SDS-PAGE - Recombinant human DNMT3B + DNMT3L protein (GST tag N-Terminus, His tag N-Terminus) (AB198133)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human DNMT3B + DNMT3L protein (GST tag N-Terminus, His tag N-Terminus) (AB198133)

10% SDS-PAGE analysis of ab198133 with Coomassie staining.

Lane 1 : ab198133
Lane 2 : Protein marker

Key facts

Purity

>85% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

Tag free

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Assayed using DNMT3B Assay Kit. Add 50 μl reaction mix containing DNMT3B/DNMT3L to wells coated with DNMT substrate. Incubate 2 hr at 37°C. Add antibody against 5-methylcytosine, then HRP-labeled secondary antibody followed by chemiluminescent detection.

Accession

Q9UBC3

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.49% Glutathione, 0.02% Potassium chloride

storage-buffer

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Complementary Products

Proteins & Peptides

AB198095

Recombinant human DNMT3A + DNMT3L protein (GST tag N-Terminus, His tag N-Terminus)

Functional Studies - Recombinant human DNMT3A + DNMT3L protein (GST tag N-Terminus, His tag N-Terminus) (AB198095)

  • 0
  • 0
Proteins & Peptides

AB271798

Human Nucleosome protein (Tag Free)

SDS-PAGE - Human Nucleosome protein (Tag Free) (AB271798)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB2851

Anti-Dnmt3b antibody

Western blot - Anti-Dnmt3b antibody (AB2851)

  • 4
  • 5
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"YPAIPAARRRPIRVLSLFDGIATGYLVLKELGIKVGKYVASEVCEESIAVGTVKHEGNIKYVNDVRNITKKNIEEWGPFDLVIGGSPCNDLSNVNPARKGLYEGTGRLFFEFYHLLNYSRPKEGDDRPFFWMFENVVAMKVGDKRDISRFLECNPVMIDAIKVSAAHRARYFWGNLPGMNRPVIASKNDKLELQDCLEYNRIAKLKKVQTITTKSNSIKQGKNQLFPVVMNGKEDVLWCTELERIFGFPVHYTDVSNMGRGARQKLLGRSWSVPVIRHLFAPLKDYFACE","proteinLength":"Fragment","predictedMolecularWeight":"59 kDa","actualMolecularWeight":null,"aminoAcidEnd":853,"aminoAcidStart":564,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q9UBC3","tags":[{"tag":"GST","terminus":"N-Terminus"}]},{"sequence":"WRSQLKAFYDRESENPLEMFETVPVWRRQPVRVLSLFEDIKKELTSLGFLESGSDPGQLKHVVDVTDTVRKDVEEWGPFDLVYGATPPLGHTCDRPPSWYLFQFHRLLQYARPKPGSPRPFFWMFVDNLVLNKEDLDVASRFLEMEPVTIPDVHGGSLQNAVRVWSNIPAIRSSRHWALVSEEELSLLAQNKQSSKLAAKWPTKLVKNCFLPLREYFKYFSTELTSSL","proteinLength":"Fragment","predictedMolecularWeight":"27 kDa","actualMolecularWeight":null,"aminoAcidEnd":387,"aminoAcidStart":160,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q9UJW3","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
True
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing (By similarity). In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Isoforms 4 and 5 are probably not functional due to the deletion of two conserved methyltransferase motifs. Functions as a transcriptional corepressor by associating with ZHX1. Required for DUX4 silencing in somatic cells (PubMed : 27153398).

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Post-translational modifications

Sumoylated.. Citrullinated by PADI4.

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Product protocols

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Target data

Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing (By similarity). In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Isoforms 4 and 5 are probably not functional due to the deletion of two conserved methyltransferase motifs. Functions as a transcriptional corepressor by associating with ZHX1. Required for DUX4 silencing in somatic cells (PubMed : 27153398).
See full target information DNMT3B

Additional targets

DNMT3L
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