Recombinant human DNMT3B + DNMT3L protein is a Human Fragment protein, in the 564 to 853 aa range, expressed in Baculovirus infected Sf9, with >85% purity and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus His tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Sequence
This product is comprised of multiple sequences see
Amino acid sequence 1
Y P A I P A A R R R P I R V L S L F D G I A T G Y L V L K E L G I K V G K Y V A S E V C E E S I A V G T V K H E G N I K Y V N D V R N I T K K N I E E W G P F D L V I G G S P C N D L S N V N P A R K G L Y E G T G R L F F E F Y H L L N Y S R P K E G D D R P F F W M F E N V V A M K V G D K R D I S R F L E C N P V M I D A I K V S A A H R A R Y F W G N L P G M N R P V I A S K N D K L E L Q D C L E Y N R I A K L K K V Q T I T T K S N S I K Q G K N Q L F P V V M N G K E D V L W C T E L E R I F G F P V H Y T D V S N M G R G A R Q K L L G R S W S V P V I R H L F A P L K D Y F A C E
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing (By similarity). In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Isoforms 4 and 5 are probably not functional due to the deletion of two conserved methyltransferase motifs. Functions as a transcriptional corepressor by associating with ZHX1. Required for DUX4 silencing in somatic cells (PubMed:27153398).
Additional Targets
DNMT3L
Alternative names
DNA (cytosine-5)-methyltransferase 3B, Dnmt3b, DNA methyltransferase HsaIIIB, DNA MTase HsaIIIB, M.HsaIIIB, DNMT3B
Recommended products
Recombinant human DNMT3B + DNMT3L protein is a Human Fragment protein, in the 564 to 853 aa range, expressed in Baculovirus infected Sf9, with >85% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus His tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Assayed using DNMT3B Assay Kit. Add 50 μl reaction mix containing DNMT3B/DNMT3L to wells coated with DNMT substrate. Incubate 2 hr at 37°C. Add antibody against 5-methylcytosine, then HRP-labeled secondary antibody followed by chemiluminescent detection.
- Accession
- Q9UBC3-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.49% Glutathione, 0.02% Potassium chloride
Sequence info
Amino acid sequence 1
- Y P A I P A A R R R P I R V L S L F D G I A T G Y L V L K E L G I K V G K Y V A S E V C E E S I A V G T V K H E G N I K Y V N D V R N I T K K N I E E W G P F D L V I G G S P C N D L S N V N P A R K G L Y E G T G R L F F E F Y H L L N Y S R P K E G D D R P F F W M F E N V V A M K V G D K R D I S R F L E C N P V M I D A I K V S A A H R A R Y F W G N L P G M N R P V I A S K N D K L E L Q D C L E Y N R I A K L K K V Q T I T T K S N S I K Q G K N Q L F P V V M N G K E D V L W C T E L E R I F G F P V H Y T D V S N M G R G A R Q K L L G R S W S V P V I R H L F A P L K D Y F A C E
- Accession
- Q9UBC3
- Protein length
- Fragment
- Predicted molecular weight
- 59 kDa
- Amino acids
- 564 to 853
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Amino acid sequence 2
- W R S Q L K A F Y D R E S E N P L E M F E T V P V W R R Q P V R V L S L F E D I K K E L T S L G F L E S G S D P G Q L K H V V D V T D T V R K D V E E W G P F D L V Y G A T P P L G H T C D R P P S W Y L F Q F H R L L Q Y A R P K P G S P R P F F W M F V D N L V L N K E D L D V A S R F L E M E P V T I P D V H G G S L Q N A V R V W S N I P A I R S S R H W A L V S E E E L S L L A Q N K Q S S K L A A K W P T K L V K N C F L P L R E Y F K Y F S T E L T S S L
- Accession
- Q9UJW3
- Protein length
- Fragment
- Predicted molecular weight
- 27 kDa
- Amino acids
- 160 to 387
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing (By similarity). In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Isoforms 4 and 5 are probably not functional due to the deletion of two conserved methyltransferase motifs. Functions as a transcriptional corepressor by associating with ZHX1. Required for DUX4 silencing in somatic cells (PubMed:27153398).
- Sequence similarities
Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.
- Post-translational modifications
Sumoylated.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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2 product images
Functional Studies - Recombinant human DNMT3B + DNMT3L protein (ab198133)
Functional assay of ab198133.
SDS-PAGE - Recombinant human DNMT3B + DNMT3L protein (ab198133)
10% SDS-PAGE analysis of ab198133 with Coomassie staining.
Lane 1: ab198133
Lane 2: Protein marker
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