Recombinant human Dnmt3L protein is a Human Full Length protein, in the 1 to 386 aa range, expressed in Baculovirus infected Sf9, with >80% purity and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >80% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M A A I P A L D P E A E P S M D V I L V G S S E L S S S V S P G T G R D L I A Y E V K A N Q R N I E D I C I C C G S L Q V H T Q H P L F E G G I C A P C K D K F L D A L F L Y D D D G Y Q S Y C S I C C S G E T L L I C G N P D C T R C Y C F E C V D S L V G P G T S G K V H A M S N W V C Y L C L P S S R S G L L Q R R R K W R S Q L K A F Y D R E S E N P L E M F E T V P V W R R Q P V R V L S L F E D I K K E L T S L G F L E S G S D P G Q L K H V V D V T D T V R K D V E E W G P F D L V Y G A T P P L G H T C D R P P S W Y L F Q F H R L L Q Y A R P K P G S P R P F F W M F V D N L V L N K E D L D V A S R F L E M E P V T I P D V H G G S L Q N A V R V W S N I P A I R S R H W A L V S E E E L S L L A Q N K Q S S K L A A K W P T K L V K N C F L P L R E Y F K Y F S T E L T S S L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Catalytically inactive regulatory factor of DNA methyltransferases that can either promote or inhibit DNA methylation depending on the context (By similarity). Essential for the function of DNMT3A and DNMT3B: activates DNMT3A and DNMT3B by binding to their catalytic domain (PubMed:17687327). Acts by accelerating the binding of DNA and S-adenosyl-L-methionine (AdoMet) to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases (PubMed:17687327). Recognizes unmethylated histone H3 lysine 4 (H3K4me0) and induces de novo DNA methylation by recruitment or activation of DNMT3 (PubMed:17687327). Plays a key role in embryonic stem cells and germ cells (By similarity). In germ cells, required for the methylation of imprinted loci together with DNMT3A (By similarity). In male germ cells, specifically required to methylate retrotransposons, preventing their mobilization (By similarity). Plays a key role in embryonic stem cells (ESCs) by acting both as an positive and negative regulator of DNA methylation (By similarity). While it promotes DNA methylation of housekeeping genes together with DNMT3A and DNMT3B, it also acts as an inhibitor of DNA methylation at the promoter of bivalent genes (By similarity). Interacts with the EZH2 component of the PRC2/EED-EZH2 complex, preventing interaction of DNMT3A and DNMT3B with the PRC2/EED-EZH2 complex, leading to maintain low methylation levels at the promoters of bivalent genes (By similarity). Promotes differentiation of ESCs into primordial germ cells by inhibiting DNA methylation at the promoter of RHOX5, thereby activating its expression (By similarity).
Alternative names
DNA (cytosine-5)-methyltransferase 3-like, DNMT3L
Recommended products
Recombinant human Dnmt3L protein is a Human Full Length protein, in the 1 to 386 aa range, expressed in Baculovirus infected Sf9, with >80% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >80% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Specific activity of ab170406 (0.25 μg) was increased 3-fold when co-incubated with 0.25 μg DNMT3L
- Accession
- Q9UJW3-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.79% Tris HCl, 0.31% Glutathione, 0.29% Sodium chloride, 0.03% EDTA, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF
Sequence info
Amino acid sequence
- M A A I P A L D P E A E P S M D V I L V G S S E L S S S V S P G T G R D L I A Y E V K A N Q R N I E D I C I C C G S L Q V H T Q H P L F E G G I C A P C K D K F L D A L F L Y D D D G Y Q S Y C S I C C S G E T L L I C G N P D C T R C Y C F E C V D S L V G P G T S G K V H A M S N W V C Y L C L P S S R S G L L Q R R R K W R S Q L K A F Y D R E S E N P L E M F E T V P V W R R Q P V R V L S L F E D I K K E L T S L G F L E S G S D P G Q L K H V V D V T D T V R K D V E E W G P F D L V Y G A T P P L G H T C D R P P S W Y L F Q F H R L L Q Y A R P K P G S P R P F F W M F V D N L V L N K E D L D V A S R F L E M E P V T I P D V H G G S L Q N A V R V W S N I P A I R S R H W A L V S E E E L S L L A Q N K Q S S K L A A K W P T K L V K N C F L P L R E Y F K Y F S T E L T S S L
- Accession
- Q9UJW3
- Protein length
- Full Length
- Predicted molecular weight
- 70 kDa
- Amino acids
- 1 to 386
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Catalytically inactive regulatory factor of DNA methyltransferases that can either promote or inhibit DNA methylation depending on the context (By similarity). Essential for the function of DNMT3A and DNMT3B: activates DNMT3A and DNMT3B by binding to their catalytic domain (PubMed:17687327). Acts by accelerating the binding of DNA and S-adenosyl-L-methionine (AdoMet) to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases (PubMed:17687327). Recognizes unmethylated histone H3 lysine 4 (H3K4me0) and induces de novo DNA methylation by recruitment or activation of DNMT3 (PubMed:17687327). Plays a key role in embryonic stem cells and germ cells (By similarity). In germ cells, required for the methylation of imprinted loci together with DNMT3A (By similarity). In male germ cells, specifically required to methylate retrotransposons, preventing their mobilization (By similarity). Plays a key role in embryonic stem cells (ESCs) by acting both as an positive and negative regulator of DNA methylation (By similarity). While it promotes DNA methylation of housekeeping genes together with DNMT3A and DNMT3B, it also acts as an inhibitor of DNA methylation at the promoter of bivalent genes (By similarity). Interacts with the EZH2 component of the PRC2/EED-EZH2 complex, preventing interaction of DNMT3A and DNMT3B with the PRC2/EED-EZH2 complex, leading to maintain low methylation levels at the promoters of bivalent genes (By similarity). Promotes differentiation of ESCs into primordial germ cells by inhibiting DNA methylation at the promoter of RHOX5, thereby activating its expression (By similarity).
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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4 product images
Functional Studies - Recombinant human Dnmt3L protein (ab170406)
The specific activity of Dnmt3L (ab170406) was increased 5-fold co0incubated with DNMT3L
SDS-PAGE - Recombinant human Dnmt3L protein (ab170406)
SDS PAGE analysis of ab170406
Functional Studies - Recombinant human Dnmt3L protein (ab170406)
Specific activity of ab170406 (0.25µg) was increased 3-fold when co-incubated with Dnmt3L (0.25µg)
SDS-PAGE - Recombinant human Dnmt3L protein (ab170406)
SDS-PAGE analysis of ab170406.
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