Recombinant Human DOK4 protein (denatured) is a Human Full Length protein, in the 1 to 326 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M A T N F S D I V K Q G Y V K M K S R K L G I Y R R C W L V F R K S S S K G P Q R L E K Y P D E K S V C L R G C P K V T E I S N V K C V T R L P K E T K R Q A V A I I F T D D S A R T F T C D S E L E A E E W Y K T L S V E C L G S R L N D I S L G E P D L L A P G V Q C E Q T D R F N V F L L P C P N L D V Y G E C K L Q I T H E N I Y L W D I H N P R V K L V S W P L C S L R R Y G R D A T R F T F E A G R M C D A G E G L Y T F Q T Q E G E Q I Y Q R V H S A T L A I A E Q H K R V L L E M E K N V R L L N K G T E H Y S Y P C T P T T M L P R S A Y W H H I T G S Q N I A E A S S Y A G E G Y G A A Q A S S E T D L L N R F I L L K P K P S Q G D S S E A K T P S Q
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK4 functions in RET-mediated neurite outgrowth and plays a positive role in activation of the MAP kinase pathway (By similarity). Putative link with downstream effectors of RET in neuronal differentiation. May be involved in the regulation of the immune response induced by T-cells.
Alternative names
Docking protein 4, Downstream of tyrosine kinase 4, Insulin receptor substrate 5, IRS-5, IRS5, DOK4
Recommended products
Recombinant Human DOK4 protein (denatured) is a Human Full Length protein, in the 1 to 326 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q8TEW6-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M A T N F S D I V K Q G Y V K M K S R K L G I Y R R C W L V F R K S S S K G P Q R L E K Y P D E K S V C L R G C P K V T E I S N V K C V T R L P K E T K R Q A V A I I F T D D S A R T F T C D S E L E A E E W Y K T L S V E C L G S R L N D I S L G E P D L L A P G V Q C E Q T D R F N V F L L P C P N L D V Y G E C K L Q I T H E N I Y L W D I H N P R V K L V S W P L C S L R R Y G R D A T R F T F E A G R M C D A G E G L Y T F Q T Q E G E Q I Y Q R V H S A T L A I A E Q H K R V L L E M E K N V R L L N K G T E H Y S Y P C T P T T M L P R S A Y W H H I T G S Q N I A E A S S Y A G E G Y G A A Q A S S E T D L L N R F I L L K P K P S Q G D S S E A K T P S Q
- Accession
- Q8TEW6
- Protein length
- Full Length
- Predicted molecular weight
- 39.4 kDa
- Amino acids
- 1 to 326
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab177645 is purified by using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA.
General info
- Function
DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK4 functions in RET-mediated neurite outgrowth and plays a positive role in activation of the MAP kinase pathway (By similarity). Putative link with downstream effectors of RET in neuronal differentiation. May be involved in the regulation of the immune response induced by T-cells.
- Sequence similarities
Belongs to the DOK family. Type B subfamily.
- Post-translational modifications
Phosphorylated on tyrosine residues in response to insulin, IGF1 or RET stimulation.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
DOK4 also known as Docking protein 4 plays an essential role in intracellular signaling pathways. Its approximate mass is 40 kDa. DOK4 is expressed in various tissues including the brain muscle tissues and epithelial cells. It serves as an adaptor or scaffold protein linking receptor tyrosine kinases with downstream signaling molecules.
- Biological function summary
DOK4 acts as a mediator in signal transduction processes. It belongs to the DOK family of proteins which do not have catalytic activity but function as part of larger signaling complexes. DOK4 influences cell differentiation proliferation and survival by mediating signals from the cell surface to the cytoplasm and nucleus. It assists in modulating the activity of various transcription factors influencing gene expression patterns.
- Pathways
DOK4 plays a significant role in the insulin signaling pathway and the MAPK/ERK pathway. It interacts with insulin receptor substrate (IRS) proteins and other signaling molecules like Ras and Raf in these pathways. These associations facilitate signal propagation affecting glucose uptake and cell growth responses. DOK4's involvement in these pathways connects it to broader physiological processes.
- Associated diseases and disorders
DOK4 is linked to metabolic disorders and certain cancers. Altered DOK4 expression affects insulin sensitivity linking it to type 2 diabetes mellitus. In cancer abnormal DOK4 expression can contribute to tumor progression through its role in proliferation signals. It interacts with proteins such as SHC1 and GRB2 which further affect disease mechanisms.
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1 product image
SDS-PAGE - Recombinant Human DOK4 protein (denatured) (ab177645)
15% SDS-PAGE: ab177645 used at 3ug.
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