Recombinant Human DPEP1/MDP protein (His tag) is a Human Fragment protein, in the 1 to 385 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M W S G W W L W P L V A V C T A D F F R D E A E R I M R D S P V I D G H N D L P W Q L L D M F N N R L Q D E R A N L T T L A G T H T N I P K L R A G F V G G Q F W S V Y T P C D T Q N K D A V R R T L E Q M D V V H R M C R M Y P E T F L Y V T S S A G I R Q A F R E G K V A S L I G V E G G H S I D S S L G V L R A L Y Q L G M R Y L T L T H S C N T P W A D N W L V D T G D S E P Q S Q G L S P F G Q R V V K E L N R L G V L I D L A H V S V A T M K A T L Q L S R A P V I F S H S S A Y S V C A S R R N V P D D V L R L V K Q T D S L V M V N F Y N N Y I S C T N K A N L S Q V A D H L D H I K E V A G A R A V G F G G D F D G V P R V P E G L E D V S K Y P D L I A E L L R R N W T E A E V K G A L A D N L L R V F E A V E Q A S N L T Q A P E E E P I P L D Q L G G S C R T H Y G Y S S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Hydrolyzes a wide range of dipeptides including the conversion of leukotriene D4 to leukotriene E4 (PubMed:2303490, PubMed:31442408, PubMed:32325220, PubMed:6334084). Hydrolyzes cystinyl-bis-glycine (cys-bis-gly) formed during glutathione degradation (PubMed:32325220). Possesses also beta lactamase activity and can hydrolyze the beta-lactam antibiotic imipenem (PubMed:32325220, PubMed:6334084). Independently of its dipeptidase activity, acts as an adhesion receptor for neutrophil recruitment from bloodstream into inflamed lungs and liver.
Alternative names
MDP, RDP, DPEP1, Dipeptidase 1, Beta-lactamase, Dehydropeptidase-I, Microsomal dipeptidase, Renal dipeptidase, hRDP
Recommended products
Recombinant Human DPEP1/MDP protein (His tag) is a Human Fragment protein, in the 1 to 385 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P16444-1
- Animal free
- No
- Carrier free
- Yes
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 100% PBS
Sequence info
Amino acid sequence
- M W S G W W L W P L V A V C T A D F F R D E A E R I M R D S P V I D G H N D L P W Q L L D M F N N R L Q D E R A N L T T L A G T H T N I P K L R A G F V G G Q F W S V Y T P C D T Q N K D A V R R T L E Q M D V V H R M C R M Y P E T F L Y V T S S A G I R Q A F R E G K V A S L I G V E G G H S I D S S L G V L R A L Y Q L G M R Y L T L T H S C N T P W A D N W L V D T G D S E P Q S Q G L S P F G Q R V V K E L N R L G V L I D L A H V S V A T M K A T L Q L S R A P V I F S H S S A Y S V C A S R R N V P D D V L R L V K Q T D S L V M V N F Y N N Y I S C T N K A N L S Q V A D H L D H I K E V A G A R A V G F G G D F D G V P R V P E G L E D V S K Y P D L I A E L L R R N W T E A E V K G A L A D N L L R V F E A V E Q A S N L T Q A P E E E P I P L D Q L G G S C R T H Y G Y S S
- Accession
- P16444
- Protein length
- Fragment
- Predicted molecular weight
- 42.5 kDa
- Amino acids
- 1 to 385
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Hydrolyzes a wide range of dipeptides including the conversion of leukotriene D4 to leukotriene E4 (PubMed:2303490, PubMed:31442408, PubMed:32325220, PubMed:6334084). Hydrolyzes cystinyl-bis-glycine (cys-bis-gly) formed during glutathione degradation (PubMed:32325220). Possesses also beta lactamase activity and can hydrolyze the beta-lactam antibiotic imipenem (PubMed:32325220, PubMed:6334084).
- Sequence similarities
Belongs to the metallo-dependent hydrolases superfamily. Peptidase M19 family.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
DPEP1 also known as MDP or dipeptidase 1 functions as a membrane-bound enzyme responsible for hydrolyzing dipeptides. It exhibits a mass of approximately 60 kDa and requires zinc ions for its enzymatic activity. Researchers often find DPEP1 in the kidney where it plays a role in the renal brush border. It is expressed also in other tissues including the intestine and can be found in the lungs and placenta. Its structure allows it to effectively act on a variety of dipeptides primarily those with hydrophobic amino acids.
- Biological function summary
DPEP1 is involved in the metabolism of leukotriene and the hydrolysis of cysteinyl leukotrienes. It is a component of the renal proximal tubule and contributes to amino acid transport and homeostasis. Its enzymatic activity modulates the levels of these substrates impacting inflammatory responses. The protein does not function alone; it often forms homomeric structures or interacts with other cellular components to perform its biological duties efficiently.
- Pathways
The enzymatic activity of DPEP1 makes it integral to the leukotriene pathway affecting both inflammatory and immune responses. It plays a role in the glutathione metabolism pathway contributing to homeostasis and detoxification processes. DPEP1's activity parallels that of other metabolic enzymes such as gamma-glutamyltransferase 1 (GGT1) which also contributes to glutathione and amino acid metabolism. These pathways showcase how DPEP1's function is tied into broader metabolic and regulatory networks.
- Associated diseases and disorders
DPEP1 is associated with conditions such as acute kidney injury and asthma. Abnormal expression or activity of DPEP1 can lead to distorted cysteinyl leukotriene levels affecting inflammation and tissue remodeling in asthmatic conditions. Additionally its expression patterns in renal tissues make it a potential marker or target in kidney disease contexts. Other proteins such as leukotriene C4 synthase interact with DPEP1 in these disease states illustrating its relevance in pathophysiological conditions.
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SDS-PAGE - Recombinant Human DPEP1/MDP protein (His tag) (ab276556)
SDS-PAGE analysis of ab276556
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