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AB159327

Recombinant Human DPF2/REQ protein

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Recombinant Human DPF2/REQ protein is a Human Full Length protein, in the 1 to 391 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

BAF45D, REQ, UBID4, DPF2, Zinc finger protein ubi-d4, Apoptosis response zinc finger protein, BRG1-associated factor 45D, Protein requiem

1 Images
SDS-PAGE - Recombinant Human DPF2/REQ protein (AB159327)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human DPF2/REQ protein (AB159327)

ab159327 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, ELISA

applications

Biologically active

No

Accession

Q92785

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as DPF2.
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Sequence info

[{"sequence":"MAAVVENVVKLLGEQYYKDAMEQCHNYNARLCAERSVRLPFLDSQTGVAQSNCYIWMEKRHRGPGLASGQLYSYPARRWRKKRRAHPPEDPRLSFPSIKPDTDQTLKKEGLISQDGSSLEALLRTDPLEKRGAPDPRVDDDSLGEFPVTNSRARKRILEPDDFLDDLDDEDYEEDTPKRRGKGKSKGKGVGSARKKLDASILEDRDKPYACDICGKRYKNRPGLSYHYAHSHLAEEEGEDKEDSQPPTPVSQRSEEQKSKKGPDGLALPNNYCDFCLGDSKINKKTGQPEELVSCSDCGRSGHPSCLQFTPVMMAAVKTYRWQCIECKCCNICGTSENDDQLLFCDDCDRGYHMYCLTPSMSEPPEGSWSCHLCLDLLKEKASIYQNQNSS","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":391,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q92785","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DPF2 also known as REQ is a protein that plays a role in chromatin remodeling an important process in gene expression regulation. The molecular weight of DPF2 is approximately 48 kDa. This protein is ubiquitously expressed in various tissues indicating its broad functional roles across different cell types. DPF2 participates actively in the modification of chromatin structure facilitating accessibility of DNA for transcription factors and other regulatory proteins.
Biological function summary

DPF2 acts as a component of the BAF complex which is a multi-subunit chromatin remodeling complex. The BAF complex modifies the structure of chromatin to regulate gene transcription affecting gene expression outcomes. DPF2 helps recruit other important proteins to the BAF complex contributing to the control of gene transcription dynamics during cell differentiation and development. Its function is essential in maintaining proper chromatin state which is important for cellular identity and function.

Pathways

DPF2 interacts significantly within the Wnt and Notch signaling pathways. In the Wnt pathway DPF2 contributes to the regulation of genes involved in cell proliferation differentiation and apoptosis. This protein also interfaces with related pathways by influencing the activity of other proteins such as beta-catenin in the Wnt pathway. In the Notch signaling pathway DPF2 modulates gene expression by affecting chromatin structure which influences cellular processes like proliferation and differentiation.

DPF2 has correlations with leukemia and other cancers. Abnormal expression or mutations of DPF2 possibly affect the balance of chromatin remodeling leading to misregulation of genes that control cell growth and survival. This protein potentially interacts with proteins such as MLL found in mixed lineage leukemia linking DPF2 to pathways that when dysregulated result in oncogenic transformation. Understanding DPF2's role in these disorders might offer novel therapeutic targets or diagnostic markers in oncology.
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Specifications

Form

Liquid

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General info

Function

Plays an active role in transcriptional regulation by binding modified histones H3 and H4 (PubMed : 27775714, PubMed : 28533407). Is a negative regulator of myeloid differentiation of hematopoietic progenitor cells (PubMed : 28533407). Might also have a role in the development and maturation of lymphoid cells (By similarity). Involved in the regulation of non-canonical NF-kappa-B pathway (PubMed : 20460684).

Sequence similarities

Belongs to the requiem/DPF family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Plays an active role in transcriptional regulation by binding modified histones H3 and H4 (PubMed : 27775714, PubMed : 28533407). Is a negative regulator of myeloid differentiation of hematopoietic progenitor cells (PubMed : 28533407). Might also have a role in the development and maturation of lymphoid cells (By similarity). Involved in the regulation of non-canonical NF-kappa-B pathway (PubMed : 20460684).
See full target information DPF2
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Complementary Products

Primary Antibodies

AB134942

Anti-DPF2/REQ antibody [EPR9206(B)]

RabMAb|Recombinant|KO Validated|Trial Size

Western blot - Anti-DPF2/REQ antibody [EPR9206(B)] (AB134942)

  • 0
  • 0
Cell Lines & Lysates

AB270496

Human DPF2 knockout A-431 cell lysate

Western blot - Human DPF2 knockout A-431 cell lysate (AB270496)

  • 0
  • 0
Cell Lines & Lysates

AB270473

Human DPF2 knockout A-431 cell line

Advanced Validation

Western blot - Human DPF2 knockout A-431 cell line (AB270473)

  • 0
  • 0

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