Recombinant human DPP4 protein is a Human Full Length protein, in the 39 to 766 aa range, expressed in Baculovirus infected BTI-TN-5B1-4, with >95% purity and suitable for WB, SDS-PAGE.
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- Biomolecules & therapeutics 29:154-1652020Effects of the Antidiabetic Drugs Evogliptin and Sitagliptin on the Immune Function of CD26/DPP4 in Th1 Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHyunyee Yoon,Ji Hyun Sung,Moon Jung SongPubMed 33148870
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34:3677-36922020Deciphering the functional heterogeneity of skin fibroblasts using single-cell RNA sequencing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVera Vorstandlechner,Maria Laggner,Polina Kalinina,Werner Haslik,Christine Radtke,Lisa Shaw,Beate Maria Lichtenberger,Erwin Tschachler,Hendrik Jan Ankersmit,Michael MildnerPubMed 31930613
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Baculovirus infected BTI-TN-5B1-4 cells
- Tags
- His tag C-Terminus
- Applications
- WB, SDS-PAGE
- Biologically active
- Yes
Amino acid sequence
S R K T Y T L T D Y L K N T Y R L K L Y S L R W I S D H E Y L Y K Q E N N I L V F N A E Y G N S S V F L E N S T F D E F G H S I N D Y S I S P D G Q F I L L E Y N Y V K Q W R H S Y T A S Y D I Y D L N K R Q L I T E E R I P N N T Q W V T W S P V G H K L A Y V W N N D I Y V K I E P N L P S Y R I T W T G K E D I I Y N G I T D W V Y E E E V F S A Y S A L W W S P N G T F L A Y A Q F N D T E V P L I E Y S F Y S D E S L Q Y P K T V R V P Y P K A G A V N P T V K F F V V N T D S L S S V T N A T S I Q I T A P A S M L I G D H Y L C D V T W A T Q E R I S L Q W L R R I Q N Y S V M D I C D Y D E S S G R W N C L V A R Q H I E M S T T G W V G R F R P S E P H F T L D G N S F Y K I I S N E E G Y R H I C Y F Q I D K K D C T F I T K G T W E V I G I E A L T S D Y L Y Y I S N E Y K G M P G G R N L Y K I Q L S D Y T K V T C L S C E L N P E R C Q Y Y S V S F S K E A K Y Y Q L R C S G P G L P L Y T L H S S V N D K G L R V L E D N S A L D K M L Q N V Q M P S K K L D F I I L N E T K F W Y Q M I L P P H F D K S K K Y P L L L D V Y A G P C S Q K A D T V F R L N W A T Y L A S T E N I I V A S F D G R G S G Y Q G D K I M H A I N R R L G T F E V E D Q I E A A R Q F S K M G F V D N K R I A I W G W S Y G G Y V T S M V L G S G S G V F K C G I A V A P V S R W E Y Y D S V Y T E R Y M G L P T P E D N L D H Y R N S T V M S R A E N F K Q V E Y L L I H G T A D D N V H F Q Q S A Q I S K A L V D V G V D F Q A M W Y T D E D H G I A S S T A H Q H I Y T H M S H F I K Q C F S L P S G R L V P R G S H H H H H H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application WB | Reactivity Reacts | Dilution info - | Notes ab79138 can be used as a WB positive control in conjunction with ab86806. |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes On SDS-PAGE under denatured condition, apparent molecular weight of glycosylated CD26 protein will be approximately 90kDa. |
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Target data
Function
Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation (PubMed:10900005, PubMed:10951221, PubMed:11772392, PubMed:17287217). Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC (PubMed:10900005, PubMed:10951221, PubMed:11772392, PubMed:14691230). Its binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner (PubMed:17287217). Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion (PubMed:11772392). In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM (PubMed:10593948, PubMed:16651416). May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation (PubMed:18708048). When overexpressed, enhanced cell proliferation, a process inhibited by GPC3 (PubMed:17549790). Acts also as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones such as brain natriuretic peptide 32 (PubMed:10570924, PubMed:16254193). Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline (PubMed:10593948). (Microbial infection) Acts as a receptor for human coronavirus MERS-CoV-2.
Alternative names
CD26, ADCP2, DPP4, Dipeptidyl peptidase 4, ADABP, Adenosine deaminase complexing protein 2, Dipeptidyl peptidase IV, T-cell activation antigen CD26, TP103, ADCP-2, DPP IV
Recommended products
Recombinant human DPP4 protein is a Human Full Length protein, in the 39 to 766 aa range, expressed in Baculovirus infected BTI-TN-5B1-4, with >95% purity and suitable for WB, SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Baculovirus infected BTI-TN-5B1-4 cells
- Tags
- His tag C-Terminus
- Applications
- WB, SDS-PAGE
- Biologically active
- Yes
- Biological activity
- Specific activity is > 200unit/mg, One unit produces 1.0 umole of p-Nitroaniline from Gly-Pro- p-Nitroaniline per minute at pH 8.0 at 37°C.
- Accession
- P27487-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0292% EDTA
Sequence info
Amino acid sequence
- S R K T Y T L T D Y L K N T Y R L K L Y S L R W I S D H E Y L Y K Q E N N I L V F N A E Y G N S S V F L E N S T F D E F G H S I N D Y S I S P D G Q F I L L E Y N Y V K Q W R H S Y T A S Y D I Y D L N K R Q L I T E E R I P N N T Q W V T W S P V G H K L A Y V W N N D I Y V K I E P N L P S Y R I T W T G K E D I I Y N G I T D W V Y E E E V F S A Y S A L W W S P N G T F L A Y A Q F N D T E V P L I E Y S F Y S D E S L Q Y P K T V R V P Y P K A G A V N P T V K F F V V N T D S L S S V T N A T S I Q I T A P A S M L I G D H Y L C D V T W A T Q E R I S L Q W L R R I Q N Y S V M D I C D Y D E S S G R W N C L V A R Q H I E M S T T G W V G R F R P S E P H F T L D G N S F Y K I I S N E E G Y R H I C Y F Q I D K K D C T F I T K G T W E V I G I E A L T S D Y L Y Y I S N E Y K G M P G G R N L Y K I Q L S D Y T K V T C L S C E L N P E R C Q Y Y S V S F S K E A K Y Y Q L R C S G P G L P L Y T L H S S V N D K G L R V L E D N S A L D K M L Q N V Q M P S K K L D F I I L N E T K F W Y Q M I L P P H F D K S K K Y P L L L D V Y A G P C S Q K A D T V F R L N W A T Y L A S T E N I I V A S F D G R G S G Y Q G D K I M H A I N R R L G T F E V E D Q I E A A R Q F S K M G F V D N K R I A I W G W S Y G G Y V T S M V L G S G S G V F K C G I A V A P V S R W E Y Y D S V Y T E R Y M G L P T P E D N L D H Y R N S T V M S R A E N F K Q V E Y L L I H G T A D D N V H F Q Q S A Q I S K A L V D V G V D F Q A M W Y T D E D H G I A S S T A H Q H I Y T H M S H F I K Q C F S L P S G R L V P R G S H H H H H H
- Accession
- P27487
- Protein length
- Full Length
- Amino acids
- 39 to 766
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified
General info
- Function
Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation (PubMed:10900005, PubMed:10951221, PubMed:11772392, PubMed:17287217). Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC (PubMed:10900005, PubMed:10951221, PubMed:11772392, PubMed:14691230). Its binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner (PubMed:17287217). Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion (PubMed:11772392). In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM (PubMed:10593948, PubMed:16651416). May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation (PubMed:18708048). When overexpressed, enhanced cell proliferation, a process inhibited by GPC3 (PubMed:17549790). Acts also as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones such as brain natriuretic peptide 32 (PubMed:10570924, PubMed:16254193). Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline (PubMed:10593948).
- Sequence similarities
Belongs to the peptidase S9B family. DPPIV subfamily.
- Post-translational modifications
The soluble form (Dipeptidyl peptidase 4 soluble form also named SDPP) derives from the membrane form (Dipeptidyl peptidase 4 membrane form also named MDPP) by proteolytic processing.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Dipeptidyl peptidase 4 also known as DPP4 or CD26 functions as a serine exopeptidase. This enzyme is involved in cleaving dipeptides from the N-terminus of polypeptides. DPP4 with a mass of approximately 110 kDa is expressed in various tissues such as the liver intestines kidney and lymphocytes. It exists in both a membrane-bound form and a soluble form in blood plasma.
- Biological function summary
DPP4 plays a significant role in glucose metabolism and immune function. The enzyme interacts with members of the immune system and regulates proteins such as chemokines growth factors and neuropeptides. DPP4 acts independently and is not known to be part of a larger complex. It modulates biological processes by inactivating peptide hormones and signaling molecules which impacts glucose homeostasis and immune responses.
- Pathways
DPP4 impacts the incretin signaling pathway and the immune response pathway. Incretin hormones like glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP) are substrates of DPP4 and play important roles in glucose metabolism. DPP4 also interacts with proteins such as adenosine deaminase (ADA) which impacts T-cell function and immune regulation. The regulation of these pathways by DPP4 influences metabolic and immune health.
- Associated diseases and disorders
DPP4 involvement is relevant in type 2 diabetes and immunological disorders. In type 2 diabetes DPP4 inhibitors modulate the incretin hormones improving glycemic control. Proteins like GLP-1 are affected by DPP4 activity connecting it to diabetes management. In autoimmune diseases altered DPP4 expression can impact immune cell functions influencing conditions such as rheumatoid arthritis. Through its effects on ADA and associated pathways DPP4 can influence both metabolic and immune-related disorders.
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3 product images
SDS-PAGE - Recombinant human DPP4 protein (ab79138)
10% SDS-PAGE showing ab79138 at approximately 90kDa (2μg).
Functional Studies - Recombinant human DPP4 protein (ab79138)
Optical density was measured at 405 nm after incubating enzyme solution with 1mM of p-nitroanilid as a substrate.
Western blot - Recombinant human DPP4 protein (ab79138)
All lanes: Anti-CD26 antibody (ab86806) at 1 µg/mL
Lane 1: Western blot - Recombinant human DPP4 protein (ab79138) at 0.1 µg
Lane 2: Western blot - Recombinant human DPP4 protein (ab79138) at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 10s
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