Recombinant Human DPP4 Protein (Active) is a Human Fragment protein, in the 29 to 766 aa range, expressed in HEK 293, with >=95% purity and <= 0.005 EU/µg endotoxin level.
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Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Biologically active
- Yes
Amino acid sequence
N K G T D D A T A D S R K T Y T L T D Y L K N T Y R L K L Y S L R W I S D H E Y L Y K Q E N N I L V F N A E Y G N S S V F L E N S T F D E F G H S I N D Y S I S P D G Q F I L L E Y N Y V K Q W R H S Y T A S Y D I Y D L N K R Q L I T E E R I P N N T Q W V T W S P V G H K L A Y V W N N D I Y V K I E P N L P S Y R I T W T G K E D I I Y N G I T D W V Y E E E V F S A Y S A L W W S P N G T F L A Y A Q F N D T E V P L I E Y S F Y S D E S L Q Y P K T V R V P Y P K A G A V N P T V K F F V V N T D S L S S V T N A T S I Q I T A P A S M L I G D H Y L C D V T W A T Q E R I S L Q W L R R I Q N Y S V M D I C D Y D E S S G R W N C L V A R Q H I E M S T T G W V G R F R P S E P H F T L D G N S F Y K I I S N E E G Y R H I C Y F Q I D K K D C T F I T K G T W E V I G I E A L T S D Y L Y Y I S N E Y K G M P G G R N L Y K I Q L S D Y T K V T C L S C E L N P E R C Q Y Y S V S F S K E A K Y Y Q L R C S G P G L P L Y T L H S S V N D K G L R V L E D N S A L D K M L Q N V Q M P S K K L D F I I L N E T K F W Y Q M I L P P H F D K S K K Y P L L L D V Y A G P C S Q K A D T V F R L N W A T Y L A S T E N I I V A S F D G R G S G Y Q G D K I M H A I N R R L G T F E V E D Q I E A A R Q F S K M G F V D N K R I A I W G W S Y G G Y V T S M V L G S G S G V F K C G I A V A P V S R W E Y Y D S V Y T E R Y M G L P T P E D N L D H Y R N S T V M S R A E N F K Q V E Y L L I H G T A D D N V H F Q Q S A Q I S K A L V D V G V D F Q A M W Y T D E D H G I A S S T A H Q H I Y T H M S H F I K Q C F S L P
Target data
Function
Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation (PubMed:10900005, PubMed:10951221, PubMed:11772392, PubMed:17287217). Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC (PubMed:10900005, PubMed:10951221, PubMed:11772392, PubMed:14691230). Its binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner (PubMed:17287217). Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion (PubMed:11772392). In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM (PubMed:10593948, PubMed:16651416). May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation (PubMed:18708048). When overexpressed, enhanced cell proliferation, a process inhibited by GPC3 (PubMed:17549790). Acts also as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones such as brain natriuretic peptide 32 (PubMed:10570924, PubMed:16254193). Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline (PubMed:10593948). (Microbial infection) Acts as a receptor for human coronavirus MERS-CoV-2.
Alternative names
CD26, ADCP2, DPP4, Dipeptidyl peptidase 4, ADABP, Adenosine deaminase complexing protein 2, Dipeptidyl peptidase IV, T-cell activation antigen CD26, TP103, ADCP-2, DPP IV
Recommended products
Recombinant Human DPP4 Protein (Active) is a Human Fragment protein, in the 29 to 766 aa range, expressed in HEK 293, with >=95% purity and <= 0.005 EU/µg endotoxin level.
Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Biologically active
- Yes
- Biological activity
- Fully biologically active determined by its ability to cleave fluorogenic peptide substrate H-Gly-Pro-7-amido-4-methylcoumarin (GP-AMC).
Specific activity is ≥ 8900 pmol/min/ug. - Accession
- P27487-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- N K G T D D A T A D S R K T Y T L T D Y L K N T Y R L K L Y S L R W I S D H E Y L Y K Q E N N I L V F N A E Y G N S S V F L E N S T F D E F G H S I N D Y S I S P D G Q F I L L E Y N Y V K Q W R H S Y T A S Y D I Y D L N K R Q L I T E E R I P N N T Q W V T W S P V G H K L A Y V W N N D I Y V K I E P N L P S Y R I T W T G K E D I I Y N G I T D W V Y E E E V F S A Y S A L W W S P N G T F L A Y A Q F N D T E V P L I E Y S F Y S D E S L Q Y P K T V R V P Y P K A G A V N P T V K F F V V N T D S L S S V T N A T S I Q I T A P A S M L I G D H Y L C D V T W A T Q E R I S L Q W L R R I Q N Y S V M D I C D Y D E S S G R W N C L V A R Q H I E M S T T G W V G R F R P S E P H F T L D G N S F Y K I I S N E E G Y R H I C Y F Q I D K K D C T F I T K G T W E V I G I E A L T S D Y L Y Y I S N E Y K G M P G G R N L Y K I Q L S D Y T K V T C L S C E L N P E R C Q Y Y S V S F S K E A K Y Y Q L R C S G P G L P L Y T L H S S V N D K G L R V L E D N S A L D K M L Q N V Q M P S K K L D F I I L N E T K F W Y Q M I L P P H F D K S K K Y P L L L D V Y A G P C S Q K A D T V F R L N W A T Y L A S T E N I I V A S F D G R G S G Y Q G D K I M H A I N R R L G T F E V E D Q I E A A R Q F S K M G F V D N K R I A I W G W S Y G G Y V T S M V L G S G S G V F K C G I A V A P V S R W E Y Y D S V Y T E R Y M G L P T P E D N L D H Y R N S T V M S R A E N F K Q V E Y L L I H G T A D D N V H F Q Q S A Q I S K A L V D V G V D F Q A M W Y T D E D H G I A S S T A H Q H I Y T H M S H F I K Q C F S L P
- Accession
- P27487
- Protein length
- Fragment
- Amino acids
- 29 to 766
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Function
Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation (PubMed:10900005, PubMed:10951221, PubMed:11772392, PubMed:17287217). Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC (PubMed:10900005, PubMed:10951221, PubMed:11772392, PubMed:14691230). Its binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner (PubMed:17287217). Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion (PubMed:11772392). In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM (PubMed:10593948, PubMed:16651416). May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation (PubMed:18708048). When overexpressed, enhanced cell proliferation, a process inhibited by GPC3 (PubMed:17549790). Acts also as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones such as brain natriuretic peptide 32 (PubMed:10570924, PubMed:16254193). Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline (PubMed:10593948).
- Sequence similarities
Belongs to the peptidase S9B family. DPPIV subfamily.
- Post-translational modifications
The soluble form (Dipeptidyl peptidase 4 soluble form also named SDPP) derives from the membrane form (Dipeptidyl peptidase 4 membrane form also named MDPP) by proteolytic processing.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Dipeptidyl peptidase 4 also known as DPP4 or CD26 functions as a serine exopeptidase. This enzyme is involved in cleaving dipeptides from the N-terminus of polypeptides. DPP4 with a mass of approximately 110 kDa is expressed in various tissues such as the liver intestines kidney and lymphocytes. It exists in both a membrane-bound form and a soluble form in blood plasma.
- Biological function summary
DPP4 plays a significant role in glucose metabolism and immune function. The enzyme interacts with members of the immune system and regulates proteins such as chemokines growth factors and neuropeptides. DPP4 acts independently and is not known to be part of a larger complex. It modulates biological processes by inactivating peptide hormones and signaling molecules which impacts glucose homeostasis and immune responses.
- Pathways
DPP4 impacts the incretin signaling pathway and the immune response pathway. Incretin hormones like glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP) are substrates of DPP4 and play important roles in glucose metabolism. DPP4 also interacts with proteins such as adenosine deaminase (ADA) which impacts T-cell function and immune regulation. The regulation of these pathways by DPP4 influences metabolic and immune health.
- Associated diseases and disorders
DPP4 involvement is relevant in type 2 diabetes and immunological disorders. In type 2 diabetes DPP4 inhibitors modulate the incretin hormones improving glycemic control. Proteins like GLP-1 are affected by DPP4 activity connecting it to diabetes management. In autoimmune diseases altered DPP4 expression can impact immune cell functions influencing conditions such as rheumatoid arthritis. Through its effects on ADA and associated pathways DPP4 can influence both metabolic and immune-related disorders.
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2 product images
Biological Activity - Recombinant Human DPP4 Protein (Active) (ab290068)
Fully biologically active determined by its ability to cleave fluorogenic peptide substrate H-Gly-Pro-7-amido-4-methylcoumarin (GP-AMC). Specific activity is ≥ 8900 pmol/min/ug.
SDS-PAGE - Recombinant Human DPP4 Protein (Active) (ab290068)
SDS-PAGE analysis of ab290068
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