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AB79662

Recombinant human DPP8 protein (GST tag N-Terminus + His tag N-Terminus)

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(1 Publication)

Recombinant human DPP8 protein (GST tag N-Terminus + His tag N-Terminus) is a Human Full Length protein, in the 1 to 898 aa range, expressed in Baculovirus infected Sf9 cells, with >80%, suitable for SDS-PAGE, WB, FuncS.

View Alternative Names

DPRP1, MSTP097, MSTP135, MSTP141, DPP8, Dipeptidyl peptidase 8, DP8, Dipeptidyl peptidase IV-related protein 1, Dipeptidyl peptidase VIII, Prolyl dipeptidase DPP8, DPRP-1, DPP VIII

3 Images
Functional Studies - Recombinant human DPP8 protein (GST tag N-Terminus + His tag N-Terminus) (AB79662)
  • FuncS

Unknown

Functional Studies - Recombinant human DPP8 protein (GST tag N-Terminus + His tag N-Terminus) (AB79662)

Specific Activity 22.4 pmol/min/μg. Determined using 10 μM Ala-Pro-AMC substrate.

Western blot - Recombinant human DPP8 protein (GST tag N-Terminus + His tag N-Terminus) (AB79662)
  • WB

Unknown

Western blot - Recombinant human DPP8 protein (GST tag N-Terminus + His tag N-Terminus) (AB79662)

All lanes:

Western blot - Anti-DPP8 antibody (<a href='/en-us/products/primary-antibodies/dpp8-antibody-ab96470'>ab96470</a>) at 1/1000 dilution

All lanes:

Western blot - Recombinant human DPP8 protein (GST tag N-Terminus + His tag N-Terminus) (ab79662) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 103 kDa,90 kDa

true

Exposure time: 3min

SDS-PAGE - Recombinant human DPP8 protein (GST tag N-Terminus + His tag N-Terminus) (AB79662)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human DPP8 protein (GST tag N-Terminus + His tag N-Terminus) (AB79662)

SDS-PAGE showing ab79662 at approximately 129kDa (4μg).

Key facts

Purity

>80% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus His tag N-Terminus

Applications

SDS-PAGE, FuncS, WB

applications

Biologically active

Yes

Biological activity

Specific Activity: 22.4 pmol/min/μg.

Accession

Q6V1X1

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 50% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.395% Tris HCl, 0.05% Sorbitan monolaurate, ethoxylated, 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Complementary Products

Proteins & Peptides

AB79621

Recombinant human DPP9 protein (GST tag N-Terminus)

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Primary Antibodies

AB42075

Anti-DPP8 antibody - Aminoterminal end

  • 4
  • 2
Proteins & Peptides

AB159886

Recombinant Human ZP2 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human ZP2 protein (GST tag N-Terminus) (AB159886)

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Proteins & Peptides

AB162402

Recombinant Human Zinc finger protein 691 (GST tag N-Terminus)

SDS-PAGE - Recombinant Human Zinc finger protein 691 (GST tag N-Terminus) (AB162402)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab79662 can be used as a WB positive control in conjunction with <a href='/en-us/products/primary-antibodies/dpp8-antibody-ab96470'>ab96470</a>.</p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Specific Activity determined using 10 µM Ala-Pro-AMC substrate</p>" } } }
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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":898,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q6V1X1","tags":[{"tag":"GST","terminus":"N-Terminus"},{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Dipeptidyl peptidase 8 also known as DPP8 is a serine protease enzyme that cleaves x-proline dipeptides from the N-termini of peptides. It has a molecular mass of around 88 kDa and shares structural similarities with DPP4. DPP8 is expressed in various tissues including the brain liver and skin but shows a notably high expression in immune cells. The enzyme is involved in regulating various physiological processes depending on its tissue distribution.
Biological function summary

DPP8 seems to play roles in modulating immune responses and cell proliferation. It functions independently and is not typically part of a larger protein complex. Studies suggest a connection between DPP8 and cellular processes such as apoptosis and inflammation although the exact biological roles require more detailed investigation.

Pathways

The peptidase activity of DPP8 integrates within metabolic and immune system pathways. In the context of metabolism it relates closely to proteins like DPP4 which influences glucose homeostasis. In immune pathways DPP8 regulates the processing of peptides affecting immune cell communication and activity sharing roles with other immune-related proteases.

DPP8 exhibits connections with inflammatory and metabolic conditions. For example its dysregulation may relate to inflammatory bowel disease (IBD) possibly affecting inflammatory cytokine release. DPP8's function parallels with DPP4 linking it indirectly to disorders like type 2 diabetes where DPP4 inhibitors are a therapeutic angle. Further exploration of DPP8's roles could indicate potential as a therapeutic target in these conditions.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Dipeptidyl peptidase that cleaves off N-terminal dipeptides from proteins having a Pro or Ala residue at position 2 (PubMed : 11012666, PubMed : 12534281, PubMed : 12662155, PubMed : 15039077, PubMed : 15664838, PubMed : 20536396, PubMed : 29382749). Acts as a key inhibitor of caspase-1-dependent monocyte and macrophage pyroptosis in resting cells by preventing activation of NLRP1 and CARD8 (PubMed : 27820798, PubMed : 29967349, PubMed : 32796818). Sequesters the cleaved C-terminal part of NLRP1 and CARD8, which respectively constitute the active part of the NLRP1 and CARD8 inflammasomes, in a ternary complex, thereby preventing their oligomerization and activation (PubMed : 33731929, PubMed : 33731932, PubMed : 34019797). The dipeptidyl peptidase activity is required to suppress NLRP1 and CARD8; however, neither NLRP1 nor CARD8 are bona fide substrates of DPP8, suggesting the existence of substrate(s) required for NLRP1 and CARD8 inhibition (By similarity).

Sequence similarities

Belongs to the peptidase S9B family. DPPIV subfamily.

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Product protocols

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Target data

Dipeptidyl peptidase that cleaves off N-terminal dipeptides from proteins having a Pro or Ala residue at position 2 (PubMed : 11012666, PubMed : 12534281, PubMed : 12662155, PubMed : 15039077, PubMed : 15664838, PubMed : 20536396, PubMed : 29382749). Acts as a key inhibitor of caspase-1-dependent monocyte and macrophage pyroptosis in resting cells by preventing activation of NLRP1 and CARD8 (PubMed : 27820798, PubMed : 29967349, PubMed : 32796818). Sequesters the cleaved C-terminal part of NLRP1 and CARD8, which respectively constitute the active part of the NLRP1 and CARD8 inflammasomes, in a ternary complex, thereby preventing their oligomerization and activation (PubMed : 33731929, PubMed : 33731932, PubMed : 34019797). The dipeptidyl peptidase activity is required to suppress NLRP1 and CARD8; however, neither NLRP1 nor CARD8 are bona fide substrates of DPP8, suggesting the existence of substrate(s) required for NLRP1 and CARD8 inhibition (By similarity).
See full target information DPP8
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The FEBS journal 282:3737-57 PubMed26175140

2015

Identification of novel dipeptidyl peptidase 9 substrates by two-dimensional differential in-gel electrophoresis.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Zhang,Sadiqa Maqsudi,Adam Rainczuk,Nadine Duffield,Josie Lawrence,Fiona M Keane,Daniela Justa-Schuch,Ruth Geiss-Friedlander,Mark D Gorrell,Andrew N Stephens
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Product promise

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