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AB182823

Recombinant Human DPT/TRAMP protein

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Recombinant Human DPT/TRAMP protein is a Human Full Length protein, in the 19 to 201 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, FuncS.

View Alternative Names

Dermatopontin, Tyrosine-rich acidic matrix protein, TRAMP, DPT

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

T7 tag N-Terminus His tag N-Terminus TEV tag N-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

No

Accession

Q07507

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.32% Tris HCl

storage-buffer

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Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB255823

Anti-DPT/TRAMP antibody [EPR22845-83]

RabMAb|Recombinant

Western blot - Anti-DPT/TRAMP antibody [EPR22845-83] (AB255823)

  • 0
  • 0
Proteins & Peptides

AB50036

Recombinant human TGF beta 1 protein (Active)

Bioactive

  • 0
  • 0
Proteins & Peptides

AB95857

Recombinant Human PIG3 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human PIG3 protein (AB95857)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>May be used for in vitro DPT mediated TGFb activity for various cell differentiation regulation study with this protein as either coating matrix protein or as soluble factor.</p><p>May be used for DPT protein – protein interaction assay.</p><p>May be used as enzymatic substrate for various proteases.</p><p>Potential biomarker protein for cancer diagnosis, such as for human oral cancer.</p><p>May be used for specific antibody production.</p>" } } }
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Product details

This product was previously labelled as Dermatopontin
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Sequence info

[{"sequence":"MASMTGGQQMGRGHHHHHHGNLYFQGGEFGQYGDYGYPYQQYHDYSDDGWVNLNRQGFSYQCPQGQVIVAVRSIFSKKEGSDRQWNYACMPTPQSLGEPTECWWEEINRAGMEWYQTCSNNGLVAGFQSRYFESVLDREWQFYCCRYSKRCPYSCWLTTEYPGHYGEEMDMISYNYDYYIRGATTTFSAVERDRQWKFIMCRMTEYDCEFANV","proteinLength":"Full Length","predictedMolecularWeight":"24 kDa","actualMolecularWeight":null,"aminoAcidEnd":201,"aminoAcidStart":19,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q07507","tags":[{"tag":"T7","terminus":"N-Terminus"},{"tag":"His","terminus":"N-Terminus"},{"tag":"TEV","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DPT also known as Dermatopontin is a protein with a molecular mass of approximately 22 kDa. It is expressed in various tissues with high expression levels in the skin heart and lungs. Dermatopontin interacts with extracellular matrix components and influences cell adhesion and matrix organization. The protein plays a role in regulating fibroblast activity and collagen binding making it important in connective tissue structure and function.
Biological function summary

Dermatopontin contributes to maintaining dermal structural integrity and facilitating wound healing. As part of a larger protein interaction network it enhances cellular communication and tissue repair processes. Dermatopontin modulates the bioavailability of TGF-beta which is essential for collagen production and tissue homeostasis. This highlights its presence in skin remodeling and repair mechanisms linking it to new tissue formation.

Pathways

Dermatopontin participates actively in the TGF-beta signaling pathway and the ECM-receptor interaction pathway. These pathways are critical for cellular communication and tissue structuring processes. The protein interacts with Integrins and Fibronectin facilitating cell-to-ECM adhesion and remodeling activities. Consequently Dermatopontin's pathway involvement supports dynamic tissue responses such as healing and functional adaptation to environmental changes.

Dermatopontin shows associations with fibrosis and keloids. These disorders feature aberrant tissue formation and scarring wherein DPT's regulation of fibroblasts and collagen dynamics plays a notable role. Additionally Dermatopontin's interactions with other proteins like Collagen VI and Decorin influence its involvement in these conditions contributing to the understanding of pathological tissue remodeling mechanisms.
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Specifications

Form

Liquid

Additional notes

Expressed in E.coli as inclusion bodies. Final product was refolded using a unique “temperature shift inclusion body refolding” technology and chromatographically purified.

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General info

Function

Seems to mediate adhesion by cell surface integrin binding. May serve as a communication link between the dermal fibroblast cell surface and its extracellular matrix environment. Enhances TGFB1 activity. Inhibits cell proliferation. Accelerates collagen fibril formation, and stabilizes collagen fibrils against low-temperature dissociation (By similarity).

Sequence similarities

Belongs to the dermatopontin family.

Post-translational modifications

Sulfated on tyrosine residue(s).

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Product protocols

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Target data

Seems to mediate adhesion by cell surface integrin binding. May serve as a communication link between the dermal fibroblast cell surface and its extracellular matrix environment. Enhances TGFB1 activity. Inhibits cell proliferation. Accelerates collagen fibril formation, and stabilizes collagen fibrils against low-temperature dissociation (By similarity).
See full target information Dermatopontin
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