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AB271489

Recombinant human DRAK2 protein (Active)

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Recombinant human DRAK2 protein (Active) is a Human Full Length protein, in the 2 to 372 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS.

View Alternative Names

DRAK2, STK17B, Serine/threonine-protein kinase 17B, DAP kinase-related apoptosis-inducing protein kinase 2

2 Images
Functional Studies - Recombinant human DRAK2 protein (Active) (AB271489)
  • FuncS

Supplier Data

Functional Studies - Recombinant human DRAK2 protein (Active) (AB271489)

Specific activity of ab271489.

SDS-PAGE - Recombinant human DRAK2 protein (Active) (AB271489)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human DRAK2 protein (Active) (AB271489)

SDS-PAGE analysis of ab271489.

Key facts

Purity

>90% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

Tag free

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Assay was done in kinase buffer containing 1 mM DTT using MRCL3 peptide substrate (0.2 mg/ml) and 20 μM ATP. Reaction was performed at 30°C for 50 mins. Amount of ATP transferred was calculated using Kinase-Glo reagent.

Accession

O94768

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.04% Sorbitan monolaurate, ethoxylated, 0.03% EDTA, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"SRRRFDCRSISGLLTTTPQIPIKMENFNNFYILTSKELGRGKFAVVRQCISKSTGQEYAAKFLKKRRRGQDCRAEILHEIAVLELAKSCPRVINLHEVYENTSEIILILEYAAGGEIFSLCLPELAEMVSENDVIRLIKQILEGVYYLHQNNIVHLDLKPQNILLSSIYPLGDIKIVDFGMSRKIGHACELREIMGTPEYLAPEILNYDPITTATDMWNIGIIAYMLLTHTSPFVGEDNQETYLNISQVNVDYSEETFSSVSQLATDFIQSLLVKNPEKRPTAEICLSHSWLQQWDFENLFHPEETSSSSQTQDHSVRSSEDKTSKSSCNGTCGDREDKENIPEDSSMVSKRFRFDDSLPNPHELVSDLLC","proteinLength":"Full Length","predictedMolecularWeight":"43 kDa","actualMolecularWeight":null,"aminoAcidEnd":372,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"O94768","tags":[]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DRAK2 also known as Death-associated protein kinase-related apoptosis-inducing protein kinase 2 is part of the serine/threonine kinase family. This protein has a molecular weight of about 48 kDa. DRAK2 expresses mostly in lymphoid tissues including the spleen and thymus. It plays a role in negative regulation during T-cell receptor signaling and is important during lymphocyte activation.
Biological function summary

The role of DRAK2 extends beyond kinase activity. It is involved in immune response modulation and can affect apoptosis in T lymphocytes. DRAK2 is not part of a larger protein complex but rather acts independently to exert its function. It influences cellular mechanisms by altering signaling pathways that impact cell survival and death.

Pathways

You find DRAK2 involved in both the T-cell receptor signaling and apoptosis pathways. It connects to the T-cell receptor pathway due to its regulation of T-cell activation and proliferation. DRAK2 activity can interact with proteins such as calmodulin and Protein Kinase C in these pathways indicating its role in signaling transduction critical for T-cell function.

DRAK2 links to autoimmune diseases and cancer. Abnormal DRAK2 expression might contribute to autoimmune conditions like multiple sclerosis by affecting T-cell autoreactivity. Its involvement in cancer emerges through regulation of apoptosis where malfunction may lead to resistance against cell death aiding cancer cell survival. In this context DRAK2 connection with proteins such as caspases plays a role in mediating apoptotic pathways in cells.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Phosphorylates myosin light chains (By similarity). Acts as a positive regulator of apoptosis.

Sequence similarities

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.

Post-translational modifications

Autophosphorylated.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Phosphorylates myosin light chains (By similarity). Acts as a positive regulator of apoptosis.
See full target information STK17B
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