Recombinant human DRAK2 protein (Active) is a Human Full Length protein, in the 2 to 372 aa range, expressed in Baculovirus infected Sf9, with =75% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- =75% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- Yes
Amino acid sequence
S R R R F D C R S I S G L L T T T P Q I P I K M E N F N N F Y I L T S K E L G R G K F A V V R Q C I S K S T G Q E Y A A K F L K K R R R G Q D C R A E I L H E I A V L E L A K S C P R V I N L H E V Y E N T S E I I L I L E Y A A G G E I F S L C L P E L A E M V S E N D V I R L I K Q I L E G V Y Y L H Q N N I V H L D L K P Q N I L L S S I Y P L G D I K I V D F G M S R K I G H A C E L R E I M G T P E Y L A P E I L N Y D P I T T A T D M W N I G I I A Y M L L T H T S P F V G E D N Q E T Y L N I S Q V N V D Y S E E T F S S V S Q L A T D F I Q S L L V K N P E K R P T A E I C L S H S W L Q Q W D F E N L F H P E E T S S S S Q T Q D H S V R S S E D K T S K S S C N G T C G D R E D K E N I P E D S S M V S K R F R F D D S L P N P H E L V S D L L C
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Phosphorylates myosin light chains (By similarity). Acts as a positive regulator of apoptosis.
Alternative names
DRAK2, STK17B, Serine/threonine-protein kinase 17B, DAP kinase-related apoptosis-inducing protein kinase 2
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Recombinant human DRAK2 protein (Active) is a Human Full Length protein, in the 2 to 372 aa range, expressed in Baculovirus infected Sf9, with =75% purity and suitable for SDS-PAGE.
Key facts
- Purity
- =75% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- Yes
- Biological activity
- Specific Activity: 15 pmole/min/μg
Assay Conditions: Assay was done in Kinase buffer containing 1 mM DTT using MRCL3 peptide substrate (0.2 mg/ml) 20 μM ATP. Reaction was done at 30°C for 45 min.
- Accession
- O94768-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 5% Glycerol (glycerin, glycerine), 1.19% HEPES, 1.13% Potassium chloride, 0.13% Magnesium chloride, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.001% Polyoxyethylene lauryl ether
Sequence info
Amino acid sequence
- S R R R F D C R S I S G L L T T T P Q I P I K M E N F N N F Y I L T S K E L G R G K F A V V R Q C I S K S T G Q E Y A A K F L K K R R R G Q D C R A E I L H E I A V L E L A K S C P R V I N L H E V Y E N T S E I I L I L E Y A A G G E I F S L C L P E L A E M V S E N D V I R L I K Q I L E G V Y Y L H Q N N I V H L D L K P Q N I L L S S I Y P L G D I K I V D F G M S R K I G H A C E L R E I M G T P E Y L A P E I L N Y D P I T T A T D M W N I G I I A Y M L L T H T S P F V G E D N Q E T Y L N I S Q V N V D Y S E E T F S S V S Q L A T D F I Q S L L V K N P E K R P T A E I C L S H S W L Q Q W D F E N L F H P E E T S S S S Q T Q D H S V R S S E D K T S K S S C N G T C G D R E D K E N I P E D S S M V S K R F R F D D S L P N P H E L V S D L L C
- Accession
- O94768
- Protein length
- Full Length
- Predicted molecular weight
- 69 kDa
- Amino acids
- 2 to 372
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Phosphorylates myosin light chains (By similarity). Acts as a positive regulator of apoptosis.
- Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.
- Post-translational modifications
Autophosphorylated.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
DRAK2 also known as Death-associated protein kinase-related apoptosis-inducing protein kinase 2 is part of the serine/threonine kinase family. This protein has a molecular weight of about 48 kDa. DRAK2 expresses mostly in lymphoid tissues including the spleen and thymus. It plays a role in negative regulation during T-cell receptor signaling and is important during lymphocyte activation.
- Biological function summary
The role of DRAK2 extends beyond kinase activity. It is involved in immune response modulation and can affect apoptosis in T lymphocytes. DRAK2 is not part of a larger protein complex but rather acts independently to exert its function. It influences cellular mechanisms by altering signaling pathways that impact cell survival and death.
- Pathways
You find DRAK2 involved in both the T-cell receptor signaling and apoptosis pathways. It connects to the T-cell receptor pathway due to its regulation of T-cell activation and proliferation. DRAK2 activity can interact with proteins such as calmodulin and Protein Kinase C in these pathways indicating its role in signaling transduction critical for T-cell function.
- Associated diseases and disorders
DRAK2 links to autoimmune diseases and cancer. Abnormal DRAK2 expression might contribute to autoimmune conditions like multiple sclerosis by affecting T-cell autoreactivity. Its involvement in cancer emerges through regulation of apoptosis where malfunction may lead to resistance against cell death aiding cancer cell survival. In this context DRAK2 connection with proteins such as caspases plays a role in mediating apoptotic pathways in cells.
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2 product images
Functional Studies - Recombinant human DRAK2 protein (Active) (ab271490)
Specific activity of ab271490 was 15 pmole/min/μg.
SDS-PAGE - Recombinant human DRAK2 protein (Active) (ab271490)
SDS-PAGE analysis of ab271490.
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