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AB152028

Recombinant Human DREF protein

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Recombinant Human DREF protein is a Human Fragment protein, in the 3 to 100 aa range, expressed in Escherichia coli, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE.

View Alternative Names

ALTE, DREF, hDREF, KIAA0785, TRAMP, ZBED1, E3 SUMO-protein ligase ZBED1, DNA replication-related element-binding factor, Putative Ac-like transposable element, Zinc finger BED domain-containing protein 1, dREF homolog

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

Escherichia coli

Tags

His tag C-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

O96006

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute in PBS

Storage buffer

pH: 7.2 Constituents: 99% Phosphate Buffer, 0.88% Sodium chloride

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

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Sequence info

[{"sequence":"NKSLESSQTDLKLVAHPRAKSKVWKYFGFDTNAEGCILQWKKIYCRICMAQIAYSGNTSNLSYHLEKNHPEEFCEFVKSNTEQMREAFATAFSKLKPE","proteinLength":"Fragment","predictedMolecularWeight":"11.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":100,"aminoAcidStart":3,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O96006","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage duration
A few weeks
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DREF also known as DNA replication-related element-binding factor is a transcription factor that regulates gene expression associated with DNA replication and cell proliferation. It is a nuclear protein with a mass of approximately 75 kDa. DREF is ubiquitously expressed in various tissues indicating its broad role in cellular processes. It binds specifically to DNA replication-related elements within the promoter regions of its target genes enabling the initiation of transcription important for cell growth and division.
Biological function summary

DREF plays an important role in regulating the expression of genes involved in DNA replication and cell cycle progression. It is known to interact with other proteins to form complexes that influence gene transcription. Through these interactions DREF coordinates the precise timing of DNA replication initiation ensuring proper cell cycle progression and maintaining genomic stability. It interacts with factors like TBP (TATA-binding protein) and contributes to the assembly of transcription machinery at replication-related promoters.

Pathways

DREF is integral to the regulation of the cell cycle and DNA replication pathways. It directly activates genes necessary for the S-phase entry. DREF interacts with the E2F family of transcription factors which also control cell cycle progression and apoptosis. The E2F/DREF pathway ensures balanced cell proliferation and programmed cell death preventing uncontrolled cell growth. DREF's role in DNA replication and cell cycle highlights its importance in maintaining cellular homeostasis.

Mutations or dysregulation of DREF can be linked to cancer and developmental disorders. Overexpression of DREF may lead to unregulated cell proliferation contributing to tumorigenesis. It associates with the p53 protein known for its role in mediating DNA repair and apoptosis following DNA damage. Dysregulation of the DREF and p53 interaction can affect the cell's response to DNA damage thereby influencing cancer progression. Understanding DREF's function in these contexts aids in developing potential therapeutic strategies targeting its pathway.
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Specifications

Form

Lyophilized

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General info

Function

Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase which sumoylates CHD3/Mi2-alpha, causing its release from DNA (PubMed : 27068747). This results in suppression of CHD3/Mi2-alpha transcription repression, increased recruitment of RNA polymerase II to gene promoters and positive regulation of transcription including H1-5 and ribosomal proteins such as : RPS6, RPL10A, and RPL12 (PubMed : 12663651, PubMed : 17209048, PubMed : 17220279, PubMed : 27068747). The resulting increased transcriptional activity drives cell proliferation (PubMed : 12663651, PubMed : 17220279). Binds to 5'-TGTCG[CT]GA[CT]A-3' consensus sequences in gene promoters of ribosomal proteins (PubMed : 12663651, PubMed : 17209048, PubMed : 17220279, PubMed : 27068747).. (Microbial infection) Binds to human adenovirus gene promoters and contributes to transcriptional repression and virus growth inhibition during early stages of infection.

Post-translational modifications

Autosumoylated with SUMO1, SUMO2, and SUMO3.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase which sumoylates CHD3/Mi2-alpha, causing its release from DNA (PubMed : 27068747). This results in suppression of CHD3/Mi2-alpha transcription repression, increased recruitment of RNA polymerase II to gene promoters and positive regulation of transcription including H1-5 and ribosomal proteins such as : RPS6, RPL10A, and RPL12 (PubMed : 12663651, PubMed : 17209048, PubMed : 17220279, PubMed : 27068747). The resulting increased transcriptional activity drives cell proliferation (PubMed : 12663651, PubMed : 17220279). Binds to 5'-TGTCG[CT]GA[CT]A-3' consensus sequences in gene promoters of ribosomal proteins (PubMed : 12663651, PubMed : 17209048, PubMed : 17220279, PubMed : 27068747).. (Microbial infection) Binds to human adenovirus gene promoters and contributes to transcriptional repression and virus growth inhibition during early stages of infection.
See full target information ZBED1
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